Strain Fitness in Escherichia coli ECRC101 around MCAODC_08525

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlaC and mlaC overlap by 1 nucleotidesmlaC and ibaG are separated by 144 nucleotidesibaG and murA are separated by 54 nucleotidesmurA and sfsB are separated by 47 nucleotidessfsB and ispB are separated by 228 nucleotides MCAODC_08510: mlaC - phospholipid-binding protein MlaC, at 1,705,951 to 1,706,586 mlaC MCAODC_08515: mlaC - phospholipid-binding protein MlaC, at 1,706,586 to 1,706,879 mlaC MCAODC_08520: ibaG - BolA family iron metabolism protein IbaG, at 1,707,024 to 1,707,293 ibaG MCAODC_08525: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,707,348 to 1,708,607 murA MCAODC_08530: sfsB - DNA-binding transcriptional regulator SfsB, at 1,708,655 to 1,708,933 sfsB MCAODC_08535: ispB - octaprenyl diphosphate synthase, at 1,709,162 to 1,710,133 ispB Position (kb) 1707 1708 1709Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1706.440 kb on + strand, within mlaCat 1706.466 kb on + strand, within mlaCat 1706.467 kb on - strand, within mlaCat 1706.504 kb on + strand, within mlaCat 1706.504 kb on + strand, within mlaCat 1706.508 kb on + strand, within mlaCat 1706.508 kb on + strand, within mlaCat 1706.508 kb on + strand, within mlaCat 1706.509 kb on - strand, within mlaCat 1706.518 kb on - strand, within mlaCat 1706.702 kb on + strand, within mlaCat 1706.702 kb on + strand, within mlaCat 1706.703 kb on - strand, within mlaCat 1706.703 kb on - strand, within mlaCat 1706.703 kb on - strand, within mlaCat 1706.717 kb on + strand, within mlaCat 1706.718 kb on - strand, within mlaCat 1706.738 kb on + strand, within mlaCat 1706.738 kb on + strand, within mlaCat 1706.816 kb on - strand, within mlaCat 1706.829 kb on + strand, within mlaCat 1706.829 kb on + strand, within mlaCat 1706.829 kb on + strand, within mlaCat 1706.829 kb on + strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.830 kb on - strand, within mlaCat 1706.831 kb on + strand, within mlaCat 1706.847 kb on + strand, within mlaCat 1706.847 kb on + strand, within mlaCat 1706.849 kb on + strand, within mlaCat 1706.952 kb on + strandat 1706.972 kb on + strandat 1706.973 kb on - strandat 1706.976 kb on + strandat 1706.997 kb on + strandat 1707.018 kb on + strandat 1707.018 kb on + strandat 1707.018 kb on + strandat 1707.047 kb on + strandat 1707.204 kb on + strand, within ibaGat 1707.204 kb on + strand, within ibaGat 1707.204 kb on + strand, within ibaGat 1707.204 kb on + strand, within ibaGat 1707.204 kb on + strand, within ibaGat 1707.206 kb on + strand, within ibaGat 1707.246 kb on + strand, within ibaGat 1707.246 kb on + strand, within ibaGat 1707.246 kb on + strand, within ibaGat 1707.246 kb on + strand, within ibaGat 1707.246 kb on + strand, within ibaGat 1707.296 kb on + strandat 1708.606 kb on - strandat 1708.674 kb on + strandat 1708.674 kb on + strandat 1708.674 kb on + strandat 1708.674 kb on + strandat 1708.675 kb on - strandat 1708.679 kb on - strandat 1708.679 kb on - strandat 1708.777 kb on + strand, within sfsBat 1708.777 kb on + strand, within sfsBat 1708.925 kb on - strandat 1708.925 kb on - strandat 1708.949 kb on - strandat 1708.954 kb on - strandat 1708.994 kb on + strandat 1708.995 kb on - strandat 1708.996 kb on + strandat 1709.032 kb on - strandat 1709.065 kb on + strandat 1709.065 kb on + strandat 1709.065 kb on + strandat 1709.065 kb on + strandat 1709.120 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,706,440 + mlaC MCAODC_08510 0.77 +0.3
1,706,466 + mlaC MCAODC_08510 0.81 +0.0
1,706,467 - mlaC MCAODC_08510 0.81 -0.9
1,706,504 + mlaC MCAODC_08510 0.87 +0.2
1,706,504 + mlaC MCAODC_08510 0.87 +1.3
1,706,508 + mlaC MCAODC_08510 0.88 +0.3
1,706,508 + mlaC MCAODC_08510 0.88 +0.9
1,706,508 + mlaC MCAODC_08510 0.88 +0.2
1,706,509 - mlaC MCAODC_08510 0.88 -2.8
1,706,518 - mlaC MCAODC_08510 0.89 +0.5
1,706,702 + mlaC MCAODC_08515 0.39 +0.2
1,706,702 + mlaC MCAODC_08515 0.39 -0.2
1,706,703 - mlaC MCAODC_08515 0.40 -1.4
1,706,703 - mlaC MCAODC_08515 0.40 -1.0
1,706,703 - mlaC MCAODC_08515 0.40 +1.0
1,706,717 + mlaC MCAODC_08515 0.45 +0.2
1,706,718 - mlaC MCAODC_08515 0.45 +0.5
1,706,738 + mlaC MCAODC_08515 0.52 -0.2
1,706,738 + mlaC MCAODC_08515 0.52 +0.5
1,706,816 - mlaC MCAODC_08515 0.78 -0.4
1,706,829 + mlaC MCAODC_08515 0.83 -0.6
1,706,829 + mlaC MCAODC_08515 0.83 +0.0
1,706,829 + mlaC MCAODC_08515 0.83 -0.3
1,706,829 + mlaC MCAODC_08515 0.83 -0.3
1,706,830 - mlaC MCAODC_08515 0.83 +1.2
1,706,830 - mlaC MCAODC_08515 0.83 -0.8
1,706,830 - mlaC MCAODC_08515 0.83 -0.1
1,706,830 - mlaC MCAODC_08515 0.83 +0.9
1,706,830 - mlaC MCAODC_08515 0.83 +0.2
1,706,830 - mlaC MCAODC_08515 0.83 +0.9
1,706,831 + mlaC MCAODC_08515 0.83 +0.5
1,706,847 + mlaC MCAODC_08515 0.89 +0.3
1,706,847 + mlaC MCAODC_08515 0.89 -0.5
1,706,849 + mlaC MCAODC_08515 0.89 -0.3
1,706,952 + -0.0
1,706,972 + -0.2
1,706,973 - +1.8
1,706,976 + +0.5
1,706,997 + -0.3
1,707,018 + +0.8
1,707,018 + +0.7
1,707,018 + -1.8
1,707,047 + -1.7
1,707,204 + ibaG MCAODC_08520 0.67 +1.5
1,707,204 + ibaG MCAODC_08520 0.67 -0.3
1,707,204 + ibaG MCAODC_08520 0.67 -1.9
1,707,204 + ibaG MCAODC_08520 0.67 -0.5
1,707,204 + ibaG MCAODC_08520 0.67 -0.6
1,707,206 + ibaG MCAODC_08520 0.67 -1.4
1,707,246 + ibaG MCAODC_08520 0.82 -2.1
1,707,246 + ibaG MCAODC_08520 0.82 -0.7
1,707,246 + ibaG MCAODC_08520 0.82 -0.6
1,707,246 + ibaG MCAODC_08520 0.82 -1.6
1,707,246 + ibaG MCAODC_08520 0.82 +0.8
1,707,296 + +0.0
1,708,606 - +0.5
1,708,674 + +0.1
1,708,674 + -2.0
1,708,674 + +0.1
1,708,674 + +0.6
1,708,675 - +1.5
1,708,679 - +0.7
1,708,679 - -0.4
1,708,777 + sfsB MCAODC_08530 0.44 +0.6
1,708,777 + sfsB MCAODC_08530 0.44 +0.6
1,708,925 - -0.1
1,708,925 - +0.2
1,708,949 - +0.6
1,708,954 - -0.3
1,708,994 + +0.1
1,708,995 - -0.6
1,708,996 + +0.6
1,709,032 - -2.3
1,709,065 + +0.8
1,709,065 + +1.0
1,709,065 + -2.4
1,709,065 + +0.2
1,709,120 - +0.4

Or see this region's nucleotide sequence