Strain Fitness in Escherichia coli ECRC101 around MCAODC_01620

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrC and thrB are separated by 0 nucleotidesthrB and thrA are separated by 1 nucleotides MCAODC_01615: thrC - threonine synthase, at 312,153 to 313,439 thrC MCAODC_01620: thrB - homoserine kinase, at 313,440 to 314,372 thrB MCAODC_01625: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 314,374 to 316,836 thrA Position (kb) 313 314 315Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 312.457 kb on + strand, within thrCat 312.632 kb on + strand, within thrCat 312.632 kb on + strand, within thrCat 312.632 kb on + strand, within thrCat 312.798 kb on - strand, within thrCat 312.806 kb on + strand, within thrCat 312.807 kb on - strand, within thrCat 312.926 kb on + strand, within thrCat 312.927 kb on - strand, within thrCat 312.927 kb on - strand, within thrCat 313.003 kb on + strand, within thrCat 313.004 kb on - strand, within thrCat 313.079 kb on + strand, within thrCat 313.121 kb on + strand, within thrCat 313.121 kb on + strand, within thrCat 313.121 kb on + strand, within thrCat 313.151 kb on - strand, within thrCat 313.253 kb on + strand, within thrCat 313.421 kb on + strandat 313.422 kb on - strandat 313.422 kb on - strandat 313.422 kb on - strandat 313.429 kb on + strandat 313.441 kb on + strandat 313.452 kb on + strandat 313.484 kb on + strandat 313.484 kb on + strandat 313.484 kb on + strandat 313.513 kb on + strandat 313.513 kb on + strandat 313.513 kb on + strandat 313.514 kb on - strandat 313.520 kb on + strandat 313.599 kb on - strand, within thrBat 313.685 kb on + strand, within thrBat 313.729 kb on + strand, within thrBat 313.730 kb on - strand, within thrBat 313.832 kb on + strand, within thrBat 313.922 kb on + strand, within thrBat 313.922 kb on + strand, within thrBat 313.923 kb on - strand, within thrBat 314.018 kb on + strand, within thrBat 314.085 kb on + strand, within thrBat 314.176 kb on + strand, within thrBat 314.268 kb on + strand, within thrBat 314.268 kb on + strand, within thrBat 314.268 kb on + strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.269 kb on - strand, within thrBat 314.336 kb on + strandat 314.337 kb on - strandat 314.384 kb on - strandat 314.384 kb on - strandat 314.401 kb on - strandat 314.401 kb on - strandat 314.401 kb on - strandat 314.404 kb on + strandat 314.404 kb on + strandat 314.405 kb on - strandat 314.405 kb on - strandat 314.405 kb on - strandat 314.484 kb on - strandat 314.486 kb on + strandat 314.486 kb on + strandat 314.487 kb on - strandat 314.487 kb on - strandat 314.495 kb on + strandat 314.496 kb on - strandat 314.592 kb on + strandat 314.603 kb on + strandat 314.604 kb on - strandat 314.604 kb on - strandat 314.604 kb on - strandat 314.683 kb on - strand, within thrAat 314.683 kb on - strand, within thrAat 314.786 kb on - strand, within thrAat 314.788 kb on + strand, within thrAat 314.788 kb on + strand, within thrAat 314.789 kb on - strand, within thrAat 314.793 kb on + strand, within thrAat 314.794 kb on - strand, within thrAat 314.794 kb on - strand, within thrAat 314.794 kb on - strand, within thrAat 314.800 kb on + strand, within thrAat 314.801 kb on - strand, within thrAat 314.801 kb on - strand, within thrAat 314.801 kb on - strand, within thrAat 314.844 kb on - strand, within thrAat 314.910 kb on + strand, within thrAat 314.911 kb on - strand, within thrAat 314.911 kb on - strand, within thrAat 314.911 kb on - strand, within thrAat 314.912 kb on + strand, within thrAat 314.912 kb on + strand, within thrAat 314.912 kb on + strand, within thrAat 314.912 kb on + strand, within thrAat 314.912 kb on + strand, within thrAat 314.912 kb on + strand, within thrAat 314.913 kb on - strand, within thrAat 314.913 kb on - strand, within thrAat 315.024 kb on - strand, within thrAat 315.024 kb on - strand, within thrAat 315.071 kb on + strand, within thrAat 315.072 kb on - strand, within thrAat 315.072 kb on - strand, within thrAat 315.075 kb on - strand, within thrAat 315.146 kb on + strand, within thrAat 315.146 kb on + strand, within thrAat 315.206 kb on + strand, within thrAat 315.240 kb on + strand, within thrAat 315.290 kb on - strand, within thrAat 315.324 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.343 kb on + strand, within thrAat 315.344 kb on - strand, within thrAat 315.344 kb on - strand, within thrAat 315.344 kb on - strand, within thrAat 315.351 kb on + strand, within thrAat 315.352 kb on - strand, within thrA

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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312,457 + thrC MCAODC_01615 0.24 -1.4
312,632 + thrC MCAODC_01615 0.37 -2.9
312,632 + thrC MCAODC_01615 0.37 -1.9
312,632 + thrC MCAODC_01615 0.37 -1.1
312,798 - thrC MCAODC_01615 0.50 -3.3
312,806 + thrC MCAODC_01615 0.51 -1.3
312,807 - thrC MCAODC_01615 0.51 -1.4
312,926 + thrC MCAODC_01615 0.60 -3.4
312,927 - thrC MCAODC_01615 0.60 -2.3
312,927 - thrC MCAODC_01615 0.60 -0.9
313,003 + thrC MCAODC_01615 0.66 -4.3
313,004 - thrC MCAODC_01615 0.66 -4.1
313,079 + thrC MCAODC_01615 0.72 -3.4
313,121 + thrC MCAODC_01615 0.75 -4.0
313,121 + thrC MCAODC_01615 0.75 -3.1
313,121 + thrC MCAODC_01615 0.75 -0.0
313,151 - thrC MCAODC_01615 0.78 -1.6
313,253 + thrC MCAODC_01615 0.85 -2.7
313,421 + -2.9
313,422 - -3.6
313,422 - -2.0
313,422 - -1.9
313,429 + -1.3
313,441 + -3.9
313,452 + -2.1
313,484 + -2.5
313,484 + -1.8
313,484 + -1.4
313,513 + -0.8
313,513 + -2.5
313,513 + -0.0
313,514 - -4.8
313,520 + -2.2
313,599 - thrB MCAODC_01620 0.17 -1.6
313,685 + thrB MCAODC_01620 0.26 -2.5
313,729 + thrB MCAODC_01620 0.31 -2.3
313,730 - thrB MCAODC_01620 0.31 -3.0
313,832 + thrB MCAODC_01620 0.42 +0.6
313,922 + thrB MCAODC_01620 0.52 -3.8
313,922 + thrB MCAODC_01620 0.52 -3.7
313,923 - thrB MCAODC_01620 0.52 -1.2
314,018 + thrB MCAODC_01620 0.62 -2.4
314,085 + thrB MCAODC_01620 0.69 +0.4
314,176 + thrB MCAODC_01620 0.79 -2.4
314,268 + thrB MCAODC_01620 0.89 -2.1
314,268 + thrB MCAODC_01620 0.89 -2.1
314,268 + thrB MCAODC_01620 0.89 -3.8
314,269 - thrB MCAODC_01620 0.89 -1.6
314,269 - thrB MCAODC_01620 0.89 -1.8
314,269 - thrB MCAODC_01620 0.89 -2.4
314,269 - thrB MCAODC_01620 0.89 -3.6
314,269 - thrB MCAODC_01620 0.89 -3.0
314,269 - thrB MCAODC_01620 0.89 -1.7
314,269 - thrB MCAODC_01620 0.89 -2.0
314,269 - thrB MCAODC_01620 0.89 -0.5
314,269 - thrB MCAODC_01620 0.89 -3.3
314,269 - thrB MCAODC_01620 0.89 -3.5
314,336 + -1.5
314,337 - -2.1
314,384 - -0.6
314,384 - -0.0
314,401 - -0.3
314,401 - -0.6
314,401 - -0.0
314,404 + -1.3
314,404 + -1.2
314,405 - -1.4
314,405 - -0.3
314,405 - -1.1
314,484 - -3.7
314,486 + -3.8
314,486 + -2.0
314,487 - -2.8
314,487 - -1.0
314,495 + -2.3
314,496 - -0.4
314,592 + -1.2
314,603 + -4.4
314,604 - -4.0
314,604 - +0.5
314,604 - -2.3
314,683 - thrA MCAODC_01625 0.13 -1.0
314,683 - thrA MCAODC_01625 0.13 -2.0
314,786 - thrA MCAODC_01625 0.17 +0.1
314,788 + thrA MCAODC_01625 0.17 -1.2
314,788 + thrA MCAODC_01625 0.17 -4.3
314,789 - thrA MCAODC_01625 0.17 -0.8
314,793 + thrA MCAODC_01625 0.17 -3.7
314,794 - thrA MCAODC_01625 0.17 -0.8
314,794 - thrA MCAODC_01625 0.17 -1.4
314,794 - thrA MCAODC_01625 0.17 -0.0
314,800 + thrA MCAODC_01625 0.17 -0.8
314,801 - thrA MCAODC_01625 0.17 -1.1
314,801 - thrA MCAODC_01625 0.17 -1.0
314,801 - thrA MCAODC_01625 0.17 -0.3
314,844 - thrA MCAODC_01625 0.19 -1.4
314,910 + thrA MCAODC_01625 0.22 -3.3
314,911 - thrA MCAODC_01625 0.22 -1.8
314,911 - thrA MCAODC_01625 0.22 -1.2
314,911 - thrA MCAODC_01625 0.22 -1.8
314,912 + thrA MCAODC_01625 0.22 -1.6
314,912 + thrA MCAODC_01625 0.22 -0.8
314,912 + thrA MCAODC_01625 0.22 -3.2
314,912 + thrA MCAODC_01625 0.22 -2.6
314,912 + thrA MCAODC_01625 0.22 +0.6
314,912 + thrA MCAODC_01625 0.22 -2.6
314,913 - thrA MCAODC_01625 0.22 -1.3
314,913 - thrA MCAODC_01625 0.22 -0.9
315,024 - thrA MCAODC_01625 0.26 -1.6
315,024 - thrA MCAODC_01625 0.26 -2.3
315,071 + thrA MCAODC_01625 0.28 -0.4
315,072 - thrA MCAODC_01625 0.28 -2.5
315,072 - thrA MCAODC_01625 0.28 -0.6
315,075 - thrA MCAODC_01625 0.28 -0.7
315,146 + thrA MCAODC_01625 0.31 -1.8
315,146 + thrA MCAODC_01625 0.31 -2.8
315,206 + thrA MCAODC_01625 0.34 -3.1
315,240 + thrA MCAODC_01625 0.35 -2.8
315,290 - thrA MCAODC_01625 0.37 -2.3
315,324 + thrA MCAODC_01625 0.39 -2.3
315,343 + thrA MCAODC_01625 0.39 -1.1
315,343 + thrA MCAODC_01625 0.39 -3.9
315,343 + thrA MCAODC_01625 0.39 -1.9
315,343 + thrA MCAODC_01625 0.39 -1.4
315,343 + thrA MCAODC_01625 0.39 -1.7
315,343 + thrA MCAODC_01625 0.39 -2.1
315,343 + thrA MCAODC_01625 0.39 -0.0
315,344 - thrA MCAODC_01625 0.39 -2.0
315,344 - thrA MCAODC_01625 0.39 -0.1
315,344 - thrA MCAODC_01625 0.39 -3.1
315,351 + thrA MCAODC_01625 0.40 -2.0
315,352 - thrA MCAODC_01625 0.40 +0.3

Or see this region's nucleotide sequence