Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_01365 and MCAODC_01370 are separated by 2 nucleotides MCAODC_01370 and MCAODC_01375 are separated by 85 nucleotides MCAODC_01375 and kefC are separated by 80 nucleotides kefC and kefF overlap by 8 nucleotides kefF and yaaU are separated by 107 nucleotides
MCAODC_01365: MCAODC_01365 - plasmid maintenance protein CcdB, at 261,843 to 262,157
_01365
MCAODC_01370: MCAODC_01370 - antitoxin, at 262,160 to 262,393
_01370
MCAODC_01375: MCAODC_01375 - Dihydrofolate reductase, at 262,479 to 263,069
_01375
MCAODC_01380: kefC - glutathione-regulated potassium-efflux system protein KefC, at 263,150 to 265,012
kefC
MCAODC_01385: kefF - glutathione-regulated potassium-efflux system oxidoreductase KefF, at 265,005 to 265,535
kefF
MCAODC_01390: yaaU - Putative metabolite transport protein YaaU, at 265,643 to 266,974
yaaU
Position (kb) 263
264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 262.161 kb on + strand at 262.225 kb on + strand, within MCAODC_01370 at 262.310 kb on - strand, within MCAODC_01370 at 262.313 kb on + strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.341 kb on - strand, within MCAODC_01370 at 262.419 kb on - strand at 262.424 kb on + strand at 262.427 kb on + strand at 262.428 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.467 kb on + strand at 262.467 kb on + strand at 262.468 kb on - strand at 262.468 kb on - strand at 262.470 kb on + strand at 262.472 kb on + strand at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.997 kb on + strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 263.048 kb on + strand at 263.050 kb on + strand at 263.118 kb on + strand at 263.118 kb on + strand at 263.119 kb on - strand at 263.125 kb on - strand at 263.151 kb on + strand at 263.195 kb on - strand at 263.195 kb on - strand at 263.200 kb on + strand at 263.237 kb on + strand at 263.276 kb on + strand at 263.277 kb on - strand at 263.277 kb on - strand at 263.299 kb on - strand at 263.299 kb on - strand at 263.360 kb on - strand, within kefC at 263.362 kb on + strand, within kefC at 263.414 kb on + strand, within kefC at 263.505 kb on + strand, within kefC at 263.505 kb on + strand, within kefC at 263.505 kb on + strand, within kefC at 263.533 kb on + strand, within kefC at 263.687 kb on - strand, within kefC at 263.695 kb on + strand, within kefC at 263.762 kb on + strand, within kefC at 263.763 kb on - strand, within kefC at 263.763 kb on - strand, within kefC at 263.807 kb on + strand, within kefC at 263.857 kb on + strand, within kefC at 264.018 kb on - strand, within kefC at 264.046 kb on + strand, within kefC at 264.046 kb on + strand, within kefC at 264.162 kb on - strand, within kefC at 264.209 kb on - strand, within kefC at 264.264 kb on - strand, within kefC at 264.264 kb on - strand, within kefC at 264.264 kb on - strand, within kefC at 264.356 kb on - strand, within kefC at 264.412 kb on - strand, within kefC at 264.412 kb on - strand, within kefC at 264.425 kb on + strand, within kefC at 264.455 kb on + strand, within kefC at 264.692 kb on + strand, within kefC at 264.693 kb on - strand, within kefC at 264.693 kb on - strand, within kefC at 264.693 kb on - strand, within kefC at 264.697 kb on + strand, within kefC at 264.697 kb on + strand, within kefC at 264.697 kb on + strand, within kefC at 264.698 kb on - strand, within kefC at 264.698 kb on - strand, within kefC at 264.698 kb on - strand, within kefC at 264.698 kb on - strand, within kefC at 264.806 kb on - strand, within kefC at 264.808 kb on + strand, within kefC at 264.809 kb on - strand, within kefC at 264.850 kb on + strand at 264.850 kb on + strand at 264.851 kb on - strand at 264.892 kb on + strand at 264.941 kb on + strand at 264.942 kb on - strand at 264.953 kb on + strand at 264.979 kb on - strand at 265.000 kb on + strand at 265.000 kb on + strand at 265.001 kb on - strand at 265.001 kb on - strand at 265.046 kb on + strand at 265.046 kb on + strand at 265.046 kb on + strand at 265.046 kb on + strand at 265.046 kb on + strand at 265.047 kb on - strand at 265.047 kb on - strand at 265.048 kb on + strand at 265.048 kb on + strand at 265.048 kb on + strand at 265.088 kb on + strand, within kefF at 265.089 kb on - strand, within kefF at 265.089 kb on - strand, within kefF at 265.089 kb on - strand, within kefF at 265.089 kb on - strand, within kefF at 265.274 kb on - strand, within kefF at 265.274 kb on - strand, within kefF at 265.313 kb on + strand, within kefF at 265.421 kb on - strand, within kefF at 265.421 kb on - strand, within kefF at 265.484 kb on + strand at 265.484 kb on + strand at 265.519 kb on + strand at 265.520 kb on - strand at 265.520 kb on - strand at 265.526 kb on + strand at 265.526 kb on + strand at 265.535 kb on + strand at 265.535 kb on + strand at 265.535 kb on + strand at 265.536 kb on - strand at 265.536 kb on - strand at 265.540 kb on - strand at 265.577 kb on + strand at 265.578 kb on - strand at 265.583 kb on + strand at 265.583 kb on + strand at 265.584 kb on - strand at 265.584 kb on - strand at 265.584 kb on - strand at 265.584 kb on - strand at 265.584 kb on - strand at 265.584 kb on - strand at 265.585 kb on + strand at 265.585 kb on + strand at 265.585 kb on + strand at 265.586 kb on - strand at 265.586 kb on - strand at 265.586 kb on - strand at 265.586 kb on - strand at 265.599 kb on - strand at 265.600 kb on + strand at 265.601 kb on - strand at 265.601 kb on - strand at 265.607 kb on + strand at 265.607 kb on + strand at 265.607 kb on + strand at 265.608 kb on - strand at 265.608 kb on - strand at 265.610 kb on + strand at 265.611 kb on - strand at 265.629 kb on + strand at 265.672 kb on + strand at 265.672 kb on + strand at 265.673 kb on - strand at 265.673 kb on - strand at 265.673 kb on - strand at 265.673 kb on - strand at 265.751 kb on - strand at 265.751 kb on - strand at 265.764 kb on - strand at 265.764 kb on - strand at 265.774 kb on + strand at 265.785 kb on + strand, within yaaU at 265.785 kb on + strand, within yaaU at 265.786 kb on - strand, within yaaU at 265.789 kb on + strand, within yaaU at 265.789 kb on + strand, within yaaU at 265.790 kb on - strand, within yaaU at 265.796 kb on - strand, within yaaU at 265.805 kb on + strand, within yaaU at 265.834 kb on + strand, within yaaU at 265.841 kb on + strand, within yaaU at 265.841 kb on + strand, within yaaU at 265.841 kb on + strand, within yaaU at 265.842 kb on - strand, within yaaU at 265.849 kb on + strand, within yaaU at 265.849 kb on + strand, within yaaU at 265.850 kb on - strand, within yaaU at 265.850 kb on - strand, within yaaU at 265.897 kb on + strand, within yaaU at 265.897 kb on + strand, within yaaU at 265.898 kb on - strand, within yaaU at 265.898 kb on - strand, within yaaU at 265.898 kb on - strand, within yaaU at 265.898 kb on - strand, within yaaU at 265.902 kb on + strand, within yaaU at 265.944 kb on + strand, within yaaU at 265.944 kb on + strand, within yaaU
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 262,161 + +1.1 262,225 + MCAODC_01370 0.28 +1.1 262,310 - MCAODC_01370 0.64 -0.5 262,313 + MCAODC_01370 0.65 -0.8 262,314 - MCAODC_01370 0.66 -0.2 262,314 - MCAODC_01370 0.66 -4.2 262,341 - MCAODC_01370 0.77 +0.7 262,419 - -0.6 262,424 + +0.1 262,427 + +0.9 262,428 - +0.4 262,448 - +0.8 262,448 - +0.4 262,448 - +0.1 262,467 + +0.1 262,467 + -0.2 262,468 - -0.1 262,468 - -1.0 262,470 + +1.0 262,472 + +1.5 262,994 - MCAODC_01375 0.87 +0.1 262,994 - MCAODC_01375 0.87 -0.2 262,994 - MCAODC_01375 0.87 -1.8 262,994 - MCAODC_01375 0.87 +0.5 262,994 - MCAODC_01375 0.87 -0.5 262,997 + MCAODC_01375 0.88 +2.0 262,998 - MCAODC_01375 0.88 -0.5 262,998 - MCAODC_01375 0.88 +0.3 262,998 - MCAODC_01375 0.88 -0.6 262,998 - MCAODC_01375 0.88 +1.4 262,998 - MCAODC_01375 0.88 +0.5 262,998 - MCAODC_01375 0.88 +0.3 263,048 + -0.1 263,050 + -0.3 263,118 + -0.3 263,118 + +2.2 263,119 - -0.4 263,125 - -0.3 263,151 + +0.5 263,195 - -0.7 263,195 - -0.0 263,200 + +1.0 263,237 + +1.0 263,276 + -1.2 263,277 - +0.1 263,277 - -1.5 263,299 - +0.4 263,299 - +0.7 263,360 - kefC MCAODC_01380 0.11 +0.8 263,362 + kefC MCAODC_01380 0.11 -1.0 263,414 + kefC MCAODC_01380 0.14 +0.2 263,505 + kefC MCAODC_01380 0.19 +0.0 263,505 + kefC MCAODC_01380 0.19 -1.0 263,505 + kefC MCAODC_01380 0.19 -0.5 263,533 + kefC MCAODC_01380 0.21 +2.7 263,687 - kefC MCAODC_01380 0.29 +0.3 263,695 + kefC MCAODC_01380 0.29 -2.4 263,762 + kefC MCAODC_01380 0.33 +0.8 263,763 - kefC MCAODC_01380 0.33 +1.1 263,763 - kefC MCAODC_01380 0.33 -2.2 263,807 + kefC MCAODC_01380 0.35 +0.7 263,857 + kefC MCAODC_01380 0.38 +0.2 264,018 - kefC MCAODC_01380 0.47 +0.2 264,046 + kefC MCAODC_01380 0.48 -0.7 264,046 + kefC MCAODC_01380 0.48 -1.3 264,162 - kefC MCAODC_01380 0.54 +0.8 264,209 - kefC MCAODC_01380 0.57 +0.6 264,264 - kefC MCAODC_01380 0.60 +0.4 264,264 - kefC MCAODC_01380 0.60 -0.6 264,264 - kefC MCAODC_01380 0.60 +0.2 264,356 - kefC MCAODC_01380 0.65 +0.0 264,412 - kefC MCAODC_01380 0.68 -0.3 264,412 - kefC MCAODC_01380 0.68 -1.2 264,425 + kefC MCAODC_01380 0.68 +0.2 264,455 + kefC MCAODC_01380 0.70 +0.7 264,692 + kefC MCAODC_01380 0.83 -1.3 264,693 - kefC MCAODC_01380 0.83 +2.9 264,693 - kefC MCAODC_01380 0.83 -0.2 264,693 - kefC MCAODC_01380 0.83 -3.9 264,697 + kefC MCAODC_01380 0.83 -0.3 264,697 + kefC MCAODC_01380 0.83 +0.3 264,697 + kefC MCAODC_01380 0.83 -1.1 264,698 - kefC MCAODC_01380 0.83 +0.7 264,698 - kefC MCAODC_01380 0.83 +0.4 264,698 - kefC MCAODC_01380 0.83 -0.0 264,698 - kefC MCAODC_01380 0.83 +0.9 264,806 - kefC MCAODC_01380 0.89 -0.3 264,808 + kefC MCAODC_01380 0.89 -0.4 264,809 - kefC MCAODC_01380 0.89 +1.1 264,850 + +0.5 264,850 + +0.6 264,851 - -0.1 264,892 + -1.6 264,941 + -0.6 264,942 - +1.8 264,953 + +0.8 264,979 - +1.4 265,000 + +0.0 265,000 + +0.4 265,001 - -0.3 265,001 - +0.1 265,046 + -0.5 265,046 + -1.0 265,046 + +1.0 265,046 + -0.3 265,046 + -0.3 265,047 - +1.6 265,047 - -0.2 265,048 + +0.6 265,048 + -2.8 265,048 + -0.2 265,088 + kefF MCAODC_01385 0.16 +0.3 265,089 - kefF MCAODC_01385 0.16 -1.2 265,089 - kefF MCAODC_01385 0.16 +0.2 265,089 - kefF MCAODC_01385 0.16 +0.1 265,089 - kefF MCAODC_01385 0.16 -0.1 265,274 - kefF MCAODC_01385 0.51 +0.0 265,274 - kefF MCAODC_01385 0.51 +3.0 265,313 + kefF MCAODC_01385 0.58 +0.4 265,421 - kefF MCAODC_01385 0.78 +0.0 265,421 - kefF MCAODC_01385 0.78 +0.6 265,484 + -0.5 265,484 + +0.1 265,519 + -1.4 265,520 - -0.9 265,520 - -0.0 265,526 + -0.1 265,526 + -1.2 265,535 + +0.9 265,535 + +0.2 265,535 + -0.4 265,536 - +0.8 265,536 - -0.4 265,540 - -1.7 265,577 + -0.8 265,578 - +2.0 265,583 + +0.1 265,583 + -0.8 265,584 - +0.5 265,584 - -0.0 265,584 - +0.3 265,584 - -1.3 265,584 - -0.3 265,584 - +0.4 265,585 + -0.2 265,585 + +2.9 265,585 + +0.5 265,586 - +0.7 265,586 - +0.5 265,586 - +0.1 265,586 - -0.4 265,599 - +0.8 265,600 + +0.8 265,601 - +0.7 265,601 - +0.5 265,607 + -2.3 265,607 + +0.1 265,607 + +0.8 265,608 - -0.3 265,608 - -0.4 265,610 + -1.4 265,611 - -0.2 265,629 + +0.8 265,672 + -0.4 265,672 + +0.2 265,673 - +0.2 265,673 - +0.4 265,673 - -1.1 265,673 - +1.5 265,751 - +0.9 265,751 - +0.1 265,764 - +0.6 265,764 - -0.6 265,774 + +0.3 265,785 + yaaU MCAODC_01390 0.11 +1.3 265,785 + yaaU MCAODC_01390 0.11 -0.3 265,786 - yaaU MCAODC_01390 0.11 +1.1 265,789 + yaaU MCAODC_01390 0.11 -0.8 265,789 + yaaU MCAODC_01390 0.11 +0.3 265,790 - yaaU MCAODC_01390 0.11 +0.2 265,796 - yaaU MCAODC_01390 0.11 +0.2 265,805 + yaaU MCAODC_01390 0.12 -0.8 265,834 + yaaU MCAODC_01390 0.14 +0.0 265,841 + yaaU MCAODC_01390 0.15 -1.1 265,841 + yaaU MCAODC_01390 0.15 +1.6 265,841 + yaaU MCAODC_01390 0.15 +0.3 265,842 - yaaU MCAODC_01390 0.15 +0.4 265,849 + yaaU MCAODC_01390 0.15 -0.9 265,849 + yaaU MCAODC_01390 0.15 -0.4 265,850 - yaaU MCAODC_01390 0.16 +0.5 265,850 - yaaU MCAODC_01390 0.16 -1.4 265,897 + yaaU MCAODC_01390 0.19 -0.2 265,897 + yaaU MCAODC_01390 0.19 -0.0 265,898 - yaaU MCAODC_01390 0.19 +0.3 265,898 - yaaU MCAODC_01390 0.19 +1.0 265,898 - yaaU MCAODC_01390 0.19 -0.0 265,898 - yaaU MCAODC_01390 0.19 +0.3 265,902 + yaaU MCAODC_01390 0.19 +0.6 265,944 + yaaU MCAODC_01390 0.23 +0.5 265,944 + yaaU MCAODC_01390 0.23 -1.5
Or see this region's nucleotide sequence