Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnD and dapD are separated by 30 nucleotides dapD and yaeH are separated by 314 nucleotides yaeH and cdaR are separated by 88 nucleotides
MCAODC_00750: glnD - bifunctional uridylyltransferase/uridylyl-removing protein GlnD, at 125,074 to 127,746
glnD
MCAODC_00755: dapD - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 127,777 to 128,601
dapD
MCAODC_00760: yaeH - DUF3461 domain-containing protein, at 128,916 to 129,302
yaeH
MCAODC_00765: cdaR - DNA-binding transcriptional regulator CdaR, at 129,391 to 130,548
cdaR
Position (kb) 127
128
129 Strain fitness (log2 ratio) -2
-1
0
1
2 at 127.737 kb on + strand at 127.753 kb on + strand at 127.753 kb on + strand at 127.759 kb on + strand at 127.772 kb on + strand at 128.606 kb on - strand at 128.653 kb on + strand at 128.664 kb on - strand at 128.689 kb on - strand at 128.689 kb on - strand at 128.722 kb on + strand at 128.723 kb on - strand at 128.740 kb on + strand at 128.740 kb on + strand at 128.741 kb on - strand at 128.749 kb on + strand at 128.766 kb on + strand at 128.778 kb on - strand at 128.778 kb on - strand at 128.787 kb on - strand at 128.812 kb on - strand at 128.880 kb on + strand at 128.915 kb on + strand at 128.916 kb on - strand at 128.916 kb on - strand at 128.919 kb on + strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.945 kb on + strand at 128.945 kb on + strand at 128.946 kb on - strand at 129.013 kb on - strand, within yaeH at 129.059 kb on + strand, within yaeH at 129.060 kb on - strand, within yaeH at 129.060 kb on - strand, within yaeH at 129.060 kb on - strand, within yaeH at 129.078 kb on - strand, within yaeH at 129.113 kb on + strand, within yaeH at 129.147 kb on + strand, within yaeH at 129.147 kb on + strand, within yaeH at 129.203 kb on + strand, within yaeH at 129.209 kb on + strand, within yaeH at 129.226 kb on + strand, within yaeH at 129.226 kb on + strand, within yaeH at 129.226 kb on + strand, within yaeH at 129.226 kb on + strand, within yaeH at 129.226 kb on + strand, within yaeH at 129.227 kb on - strand, within yaeH at 129.227 kb on - strand, within yaeH at 129.227 kb on - strand, within yaeH at 129.227 kb on - strand, within yaeH at 129.275 kb on + strand at 129.296 kb on + strand at 129.387 kb on + strand at 129.387 kb on + strand at 129.393 kb on - strand at 129.422 kb on + strand at 129.422 kb on + strand at 129.422 kb on + strand at 129.423 kb on - strand at 129.423 kb on - strand at 129.423 kb on - strand at 129.423 kb on - strand at 129.423 kb on - strand at 129.475 kb on + strand at 129.477 kb on + strand at 129.483 kb on + strand at 129.484 kb on - strand at 129.501 kb on + strand at 129.502 kb on - strand at 129.504 kb on + strand at 129.505 kb on - strand at 129.505 kb on - strand at 129.505 kb on - strand at 129.514 kb on - strand, within cdaR
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 127,737 + +1.4 127,753 + -0.3 127,753 + +0.1 127,759 + +0.2 127,772 + -1.5 128,606 - -1.0 128,653 + +0.9 128,664 - +0.9 128,689 - -0.8 128,689 - +0.7 128,722 + +0.7 128,723 - +0.3 128,740 + +0.6 128,740 + +0.3 128,741 - -0.1 128,749 + +0.7 128,766 + +0.1 128,778 - +0.0 128,778 - +1.1 128,787 - +0.1 128,812 - +0.3 128,880 + +0.5 128,915 + +0.3 128,916 - +0.0 128,916 - +0.1 128,919 + -1.5 128,920 - -0.3 128,920 - +0.5 128,945 + +0.4 128,945 + +0.8 128,946 - -0.9 129,013 - yaeH MCAODC_00760 0.25 +0.2 129,059 + yaeH MCAODC_00760 0.37 -0.3 129,060 - yaeH MCAODC_00760 0.37 +1.2 129,060 - yaeH MCAODC_00760 0.37 -0.5 129,060 - yaeH MCAODC_00760 0.37 -0.5 129,078 - yaeH MCAODC_00760 0.42 +0.2 129,113 + yaeH MCAODC_00760 0.51 +1.8 129,147 + yaeH MCAODC_00760 0.60 -0.4 129,147 + yaeH MCAODC_00760 0.60 +1.5 129,203 + yaeH MCAODC_00760 0.74 -0.1 129,209 + yaeH MCAODC_00760 0.76 +0.5 129,226 + yaeH MCAODC_00760 0.80 +0.2 129,226 + yaeH MCAODC_00760 0.80 -1.0 129,226 + yaeH MCAODC_00760 0.80 +0.3 129,226 + yaeH MCAODC_00760 0.80 +0.1 129,226 + yaeH MCAODC_00760 0.80 +0.0 129,227 - yaeH MCAODC_00760 0.80 +1.1 129,227 - yaeH MCAODC_00760 0.80 +0.1 129,227 - yaeH MCAODC_00760 0.80 -0.0 129,227 - yaeH MCAODC_00760 0.80 +0.0 129,275 + +0.4 129,296 + -0.6 129,387 + +0.0 129,387 + -1.3 129,393 - -0.1 129,422 + +0.2 129,422 + +0.4 129,422 + -0.4 129,423 - -1.8 129,423 - +2.7 129,423 - +0.2 129,423 - +0.4 129,423 - -0.3 129,475 + -0.5 129,477 + -0.8 129,483 + -0.6 129,484 - -1.5 129,501 + +0.6 129,502 - -0.2 129,504 + +0.7 129,505 - +0.4 129,505 - -2.1 129,505 - +0.2 129,514 - cdaR MCAODC_00765 0.11 +0.9
Or see this region's nucleotide sequence