Experiment: Uracil 0.8921 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS05375 and A4249_RS05380 are separated by 139 nucleotides A4249_RS05380 and A4249_RS05385 overlap by 4 nucleotides A4249_RS05385 and A4249_RS05390 are separated by 25 nucleotides A4249_RS05390 and A4249_RS05395 overlap by 1 nucleotides A4249_RS05395 and A4249_RS05400 are separated by 1 nucleotides
A4249_RS05375: A4249_RS05375 - damage-inducible protein, at 1,100,791 to 1,101,546
_RS05375
A4249_RS05380: A4249_RS05380 - hypothetical protein, at 1,101,686 to 1,102,024
_RS05380
A4249_RS05385: A4249_RS05385 - DUF1810 domain-containing protein, at 1,102,021 to 1,102,461
_RS05385
A4249_RS05390: A4249_RS05390 - SOS response-associated peptidase family protein, at 1,102,487 to 1,103,194
_RS05390
A4249_RS05395: A4249_RS05395 - hypothetical protein, at 1,103,194 to 1,103,640
_RS05395
A4249_RS05400: A4249_RS05400 - hypothetical protein, at 1,103,642 to 1,103,878
_RS05400
Position (kb)
1102
1103
1104 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1101.729 kb on - strand, within A4249_RS05380 at 1101.729 kb on - strand, within A4249_RS05380 at 1101.983 kb on + strand, within A4249_RS05380 at 1101.984 kb on - strand, within A4249_RS05380 at 1101.984 kb on - strand, within A4249_RS05380 at 1101.984 kb on - strand, within A4249_RS05380 at 1102.049 kb on + strand at 1102.049 kb on + strand at 1102.050 kb on - strand at 1102.118 kb on + strand, within A4249_RS05385 at 1102.118 kb on + strand, within A4249_RS05385 at 1102.119 kb on - strand, within A4249_RS05385 at 1102.174 kb on + strand, within A4249_RS05385 at 1102.174 kb on + strand, within A4249_RS05385 at 1102.174 kb on + strand, within A4249_RS05385 at 1102.174 kb on + strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.175 kb on - strand, within A4249_RS05385 at 1102.207 kb on + strand, within A4249_RS05385 at 1102.207 kb on + strand, within A4249_RS05385 at 1102.207 kb on + strand, within A4249_RS05385 at 1102.207 kb on + strand, within A4249_RS05385 at 1102.208 kb on - strand, within A4249_RS05385 at 1102.208 kb on - strand, within A4249_RS05385 at 1102.208 kb on - strand, within A4249_RS05385 at 1102.208 kb on - strand, within A4249_RS05385 at 1102.208 kb on - strand, within A4249_RS05385 at 1102.226 kb on + strand, within A4249_RS05385 at 1102.227 kb on - strand, within A4249_RS05385 at 1102.227 kb on - strand, within A4249_RS05385 at 1102.337 kb on + strand, within A4249_RS05385 at 1102.337 kb on + strand, within A4249_RS05385 at 1102.338 kb on - strand, within A4249_RS05385 at 1102.338 kb on - strand, within A4249_RS05385 at 1102.338 kb on - strand, within A4249_RS05385 at 1102.338 kb on - strand, within A4249_RS05385 at 1102.427 kb on + strand at 1102.427 kb on + strand at 1102.427 kb on + strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.428 kb on - strand at 1102.460 kb on - strand at 1102.460 kb on - strand at 1102.467 kb on + strand at 1102.479 kb on + strand at 1102.479 kb on + strand at 1102.480 kb on - strand at 1102.480 kb on - strand at 1102.480 kb on - strand at 1102.480 kb on - strand at 1102.488 kb on + strand at 1102.488 kb on + strand at 1102.488 kb on + strand at 1102.499 kb on - strand at 1102.788 kb on + strand, within A4249_RS05390 at 1102.789 kb on - strand, within A4249_RS05390 at 1102.789 kb on - strand, within A4249_RS05390 at 1102.789 kb on - strand, within A4249_RS05390 at 1102.789 kb on - strand, within A4249_RS05390 at 1102.789 kb on - strand, within A4249_RS05390 at 1103.094 kb on + strand, within A4249_RS05390 at 1103.094 kb on + strand, within A4249_RS05390 at 1103.153 kb on + strand at 1103.153 kb on + strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.182 kb on - strand at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.246 kb on + strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.247 kb on - strand, within A4249_RS05395 at 1103.437 kb on - strand, within A4249_RS05395 at 1103.487 kb on - strand, within A4249_RS05395 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.672 kb on + strand, within A4249_RS05400 at 1103.673 kb on - strand, within A4249_RS05400 at 1103.673 kb on - strand, within A4249_RS05400 at 1103.673 kb on - strand, within A4249_RS05400 at 1103.673 kb on - strand, within A4249_RS05400 at 1103.673 kb on - strand, within A4249_RS05400 at 1103.768 kb on + strand, within A4249_RS05400 at 1103.769 kb on - strand, within A4249_RS05400 at 1103.948 kb on + strand at 1103.948 kb on + strand at 1103.948 kb on + strand at 1103.949 kb on - strand at 1103.949 kb on - strand at 1103.949 kb on - strand at 1103.949 kb on - strand at 1103.949 kb on - strand at 1103.957 kb on + strand at 1103.958 kb on - strand at 1103.973 kb on - strand at 1103.973 kb on - strand at 1104.168 kb on + strand at 1104.168 kb on + strand at 1104.169 kb on - strand at 1104.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uracil 0.8921 mM (C) remove 1,101,729 - A4249_RS05380 0.13 +0.2 1,101,729 - A4249_RS05380 0.13 -0.7 1,101,983 + A4249_RS05380 0.88 -0.6 1,101,984 - A4249_RS05380 0.88 -0.0 1,101,984 - A4249_RS05380 0.88 +0.1 1,101,984 - A4249_RS05380 0.88 +0.3 1,102,049 + -0.5 1,102,049 + -3.2 1,102,050 - -0.7 1,102,118 + A4249_RS05385 0.22 -0.8 1,102,118 + A4249_RS05385 0.22 +0.5 1,102,119 - A4249_RS05385 0.22 +0.1 1,102,174 + A4249_RS05385 0.35 +1.0 1,102,174 + A4249_RS05385 0.35 +1.1 1,102,174 + A4249_RS05385 0.35 +0.6 1,102,174 + A4249_RS05385 0.35 +0.2 1,102,175 - A4249_RS05385 0.35 -0.9 1,102,175 - A4249_RS05385 0.35 -0.9 1,102,175 - A4249_RS05385 0.35 +0.0 1,102,175 - A4249_RS05385 0.35 -0.2 1,102,175 - A4249_RS05385 0.35 -0.3 1,102,175 - A4249_RS05385 0.35 -0.1 1,102,175 - A4249_RS05385 0.35 -0.3 1,102,207 + A4249_RS05385 0.42 -0.3 1,102,207 + A4249_RS05385 0.42 -0.1 1,102,207 + A4249_RS05385 0.42 -0.1 1,102,207 + A4249_RS05385 0.42 -2.3 1,102,208 - A4249_RS05385 0.42 +0.3 1,102,208 - A4249_RS05385 0.42 -1.5 1,102,208 - A4249_RS05385 0.42 -0.5 1,102,208 - A4249_RS05385 0.42 -0.2 1,102,208 - A4249_RS05385 0.42 -1.0 1,102,226 + A4249_RS05385 0.46 +0.2 1,102,227 - A4249_RS05385 0.47 -0.1 1,102,227 - A4249_RS05385 0.47 +0.6 1,102,337 + A4249_RS05385 0.72 -0.1 1,102,337 + A4249_RS05385 0.72 +0.6 1,102,338 - A4249_RS05385 0.72 -0.9 1,102,338 - A4249_RS05385 0.72 -1.4 1,102,338 - A4249_RS05385 0.72 +0.8 1,102,338 - A4249_RS05385 0.72 +0.0 1,102,427 + +0.4 1,102,427 + +0.9 1,102,427 + -0.3 1,102,428 - -0.3 1,102,428 - +0.2 1,102,428 - +0.4 1,102,428 - -1.0 1,102,428 - +0.2 1,102,428 - -0.1 1,102,428 - -0.1 1,102,460 - +0.9 1,102,460 - +0.4 1,102,467 + -0.4 1,102,479 + +0.5 1,102,479 + +1.6 1,102,480 - -0.0 1,102,480 - +0.2 1,102,480 - -0.3 1,102,480 - +0.5 1,102,488 + -1.1 1,102,488 + -0.6 1,102,488 + +0.2 1,102,499 - +0.1 1,102,788 + A4249_RS05390 0.43 +1.0 1,102,789 - A4249_RS05390 0.43 +0.9 1,102,789 - A4249_RS05390 0.43 -0.5 1,102,789 - A4249_RS05390 0.43 -1.2 1,102,789 - A4249_RS05390 0.43 -0.3 1,102,789 - A4249_RS05390 0.43 -0.4 1,103,094 + A4249_RS05390 0.86 -0.7 1,103,094 + A4249_RS05390 0.86 -0.7 1,103,153 + -0.2 1,103,153 + -0.1 1,103,154 - -0.4 1,103,154 - +0.3 1,103,154 - -0.7 1,103,182 - +0.1 1,103,246 + A4249_RS05395 0.12 -0.3 1,103,246 + A4249_RS05395 0.12 +0.4 1,103,246 + A4249_RS05395 0.12 +0.1 1,103,246 + A4249_RS05395 0.12 -0.8 1,103,246 + A4249_RS05395 0.12 -0.8 1,103,246 + A4249_RS05395 0.12 -0.1 1,103,246 + A4249_RS05395 0.12 -0.4 1,103,246 + A4249_RS05395 0.12 -0.6 1,103,247 - A4249_RS05395 0.12 -0.1 1,103,247 - A4249_RS05395 0.12 +0.0 1,103,247 - A4249_RS05395 0.12 +0.4 1,103,247 - A4249_RS05395 0.12 -0.1 1,103,247 - A4249_RS05395 0.12 +0.1 1,103,247 - A4249_RS05395 0.12 -0.7 1,103,437 - A4249_RS05395 0.54 -0.2 1,103,487 - A4249_RS05395 0.66 +0.2 1,103,672 + A4249_RS05400 0.13 +1.0 1,103,672 + A4249_RS05400 0.13 -1.0 1,103,672 + A4249_RS05400 0.13 -0.2 1,103,672 + A4249_RS05400 0.13 -1.3 1,103,672 + A4249_RS05400 0.13 -0.7 1,103,672 + A4249_RS05400 0.13 -0.0 1,103,672 + A4249_RS05400 0.13 -1.0 1,103,672 + A4249_RS05400 0.13 -0.2 1,103,672 + A4249_RS05400 0.13 +1.6 1,103,672 + A4249_RS05400 0.13 +0.1 1,103,672 + A4249_RS05400 0.13 +0.1 1,103,673 - A4249_RS05400 0.13 +0.0 1,103,673 - A4249_RS05400 0.13 +0.5 1,103,673 - A4249_RS05400 0.13 +0.1 1,103,673 - A4249_RS05400 0.13 -0.9 1,103,673 - A4249_RS05400 0.13 +1.4 1,103,768 + A4249_RS05400 0.53 -0.3 1,103,769 - A4249_RS05400 0.54 -0.4 1,103,948 + +0.5 1,103,948 + +0.4 1,103,948 + +0.3 1,103,949 - +0.3 1,103,949 - +0.6 1,103,949 - +0.3 1,103,949 - +1.0 1,103,949 - -0.3 1,103,957 + -0.4 1,103,958 - +0.2 1,103,973 - -1.1 1,103,973 - -1.0 1,104,168 + +0.4 1,104,168 + -0.1 1,104,169 - +0.2 1,104,182 + -0.5
Or see this region's nucleotide sequence