Strain Fitness in Escherichia coli ECRC101 around MCAODC_27640

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiI and xseB are separated by 205 nucleotidesxseB and ispA overlap by 1 nucleotidesispA and dxs are separated by 24 nucleotides MCAODC_27630: thiI - tRNA sulfurtransferase, at 2,271,432 to 2,272,880 thiI MCAODC_27635: xseB - exodeoxyribonuclease VII small subunit, at 2,273,086 to 2,273,328 xseB MCAODC_27640: ispA - (2E,6E)-farnesyl diphosphate synthase, at 2,273,328 to 2,274,227 ispA MCAODC_27645: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 2,274,252 to 2,276,114 dxs Position (kb) 2273 2274 2275Strain fitness (log2 ratio) -2 -1 0 1 2at 2272.377 kb on - strand, within thiIat 2272.486 kb on - strand, within thiIat 2272.501 kb on + strand, within thiIat 2272.501 kb on + strand, within thiIat 2272.655 kb on + strand, within thiIat 2272.771 kb on + strandat 2272.771 kb on + strandat 2272.772 kb on - strandat 2272.772 kb on - strandat 2272.780 kb on - strandat 2272.871 kb on + strandat 2272.918 kb on + strandat 2272.918 kb on + strandat 2272.919 kb on - strandat 2272.919 kb on - strandat 2272.919 kb on - strandat 2272.919 kb on - strandat 2272.919 kb on - strandat 2272.920 kb on + strandat 2272.930 kb on + strandat 2272.971 kb on - strandat 2272.971 kb on - strandat 2272.971 kb on - strandat 2272.971 kb on - strandat 2272.971 kb on - strandat 2272.972 kb on + strandat 2272.973 kb on - strandat 2273.006 kb on + strandat 2273.007 kb on - strandat 2273.007 kb on - strandat 2273.007 kb on - strandat 2273.020 kb on - strandat 2273.020 kb on - strandat 2273.064 kb on + strandat 2273.085 kb on + strandat 2273.150 kb on + strand, within xseB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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2,272,377 - thiI MCAODC_27630 0.65 +1.2
2,272,486 - thiI MCAODC_27630 0.73 +1.4
2,272,501 + thiI MCAODC_27630 0.74 +1.9
2,272,501 + thiI MCAODC_27630 0.74 +0.5
2,272,655 + thiI MCAODC_27630 0.84 -0.7
2,272,771 + -1.0
2,272,771 + -0.2
2,272,772 - +0.5
2,272,772 - +0.4
2,272,780 - +1.2
2,272,871 + +0.5
2,272,918 + +0.2
2,272,918 + +0.1
2,272,919 - +0.6
2,272,919 - +1.3
2,272,919 - -0.6
2,272,919 - -0.6
2,272,919 - -0.2
2,272,920 + +2.1
2,272,930 + -0.2
2,272,971 - +0.7
2,272,971 - -0.6
2,272,971 - +0.2
2,272,971 - -0.5
2,272,971 - +0.7
2,272,972 + -1.0
2,272,973 - +0.5
2,273,006 + -0.4
2,273,007 - +1.7
2,273,007 - -1.4
2,273,007 - -0.4
2,273,020 - -1.1
2,273,020 - -0.4
2,273,064 + -2.2
2,273,085 + +0.8
2,273,150 + xseB MCAODC_27635 0.26 +0.8

Or see this region's nucleotide sequence