Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiI and xseB are separated by 205 nucleotides xseB and ispA overlap by 1 nucleotides ispA and dxs are separated by 24 nucleotides
MCAODC_27630: thiI - tRNA sulfurtransferase, at 2,271,432 to 2,272,880
thiI
MCAODC_27635: xseB - exodeoxyribonuclease VII small subunit, at 2,273,086 to 2,273,328
xseB
MCAODC_27640: ispA - (2E,6E)-farnesyl diphosphate synthase, at 2,273,328 to 2,274,227
ispA
MCAODC_27645: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 2,274,252 to 2,276,114
dxs
Position (kb)
2273
2274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2272.155 kb on - strand, within thiI at 2272.158 kb on - strand, within thiI at 2272.205 kb on - strand, within thiI at 2272.205 kb on - strand, within thiI at 2272.254 kb on - strand, within thiI at 2272.264 kb on + strand, within thiI at 2272.264 kb on + strand, within thiI at 2272.295 kb on + strand, within thiI at 2272.296 kb on - strand, within thiI at 2272.296 kb on - strand, within thiI at 2272.296 kb on - strand, within thiI at 2272.296 kb on - strand, within thiI at 2272.297 kb on + strand, within thiI at 2272.298 kb on - strand, within thiI at 2272.377 kb on - strand, within thiI at 2272.486 kb on - strand, within thiI at 2272.501 kb on + strand, within thiI at 2272.501 kb on + strand, within thiI at 2272.655 kb on + strand, within thiI at 2272.771 kb on + strand at 2272.771 kb on + strand at 2272.772 kb on - strand at 2272.772 kb on - strand at 2272.780 kb on - strand at 2272.871 kb on + strand at 2272.918 kb on + strand at 2272.918 kb on + strand at 2272.919 kb on - strand at 2272.919 kb on - strand at 2272.919 kb on - strand at 2272.919 kb on - strand at 2272.919 kb on - strand at 2272.920 kb on + strand at 2272.930 kb on + strand at 2272.971 kb on - strand at 2272.971 kb on - strand at 2272.971 kb on - strand at 2272.971 kb on - strand at 2272.971 kb on - strand at 2272.972 kb on + strand at 2272.973 kb on - strand at 2273.006 kb on + strand at 2273.007 kb on - strand at 2273.007 kb on - strand at 2273.007 kb on - strand at 2273.020 kb on - strand at 2273.020 kb on - strand at 2273.064 kb on + strand at 2273.085 kb on + strand at 2273.150 kb on + strand, within xseB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 2,272,155 - thiI MCAODC_27630 0.50 +0.5 2,272,158 - thiI MCAODC_27630 0.50 +1.6 2,272,205 - thiI MCAODC_27630 0.53 +0.2 2,272,205 - thiI MCAODC_27630 0.53 -0.2 2,272,254 - thiI MCAODC_27630 0.57 +1.1 2,272,264 + thiI MCAODC_27630 0.57 -0.3 2,272,264 + thiI MCAODC_27630 0.57 +0.8 2,272,295 + thiI MCAODC_27630 0.60 +0.1 2,272,296 - thiI MCAODC_27630 0.60 -0.5 2,272,296 - thiI MCAODC_27630 0.60 +1.7 2,272,296 - thiI MCAODC_27630 0.60 +1.4 2,272,296 - thiI MCAODC_27630 0.60 +1.1 2,272,297 + thiI MCAODC_27630 0.60 -0.5 2,272,298 - thiI MCAODC_27630 0.60 -2.0 2,272,377 - thiI MCAODC_27630 0.65 +1.2 2,272,486 - thiI MCAODC_27630 0.73 +1.4 2,272,501 + thiI MCAODC_27630 0.74 +1.9 2,272,501 + thiI MCAODC_27630 0.74 +0.5 2,272,655 + thiI MCAODC_27630 0.84 -0.7 2,272,771 + -1.0 2,272,771 + -0.2 2,272,772 - +0.5 2,272,772 - +0.4 2,272,780 - +1.2 2,272,871 + +0.5 2,272,918 + +0.2 2,272,918 + +0.1 2,272,919 - +0.6 2,272,919 - +1.3 2,272,919 - -0.6 2,272,919 - -0.6 2,272,919 - -0.2 2,272,920 + +2.1 2,272,930 + -0.2 2,272,971 - +0.7 2,272,971 - -0.6 2,272,971 - +0.2 2,272,971 - -0.5 2,272,971 - +0.7 2,272,972 + -1.0 2,272,973 - +0.5 2,273,006 + -0.4 2,273,007 - +1.7 2,273,007 - -1.4 2,273,007 - -0.4 2,273,020 - -1.1 2,273,020 - -0.4 2,273,064 + -2.2 2,273,085 + +0.8 2,273,150 + xseB MCAODC_27635 0.26 +0.8
Or see this region's nucleotide sequence