Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnS and ompF are separated by 602 nucleotides ompF and aspC are separated by 184 nucleotides
MCAODC_24765: asnS - asparagine--tRNA ligase, at 1,662,256 to 1,663,656
asnS
MCAODC_24770: ompF - porin OmpF, at 1,664,259 to 1,665,347
ompF
MCAODC_24775: aspC - aspartate transaminase, at 1,665,532 to 1,666,722
aspC
Position (kb)
1664
1665
1666 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1663.654 kb on + strand at 1663.654 kb on + strand at 1663.714 kb on - strand at 1663.725 kb on - strand at 1663.780 kb on + strand at 1663.979 kb on - strand at 1663.999 kb on - strand at 1664.125 kb on + strand at 1664.156 kb on + strand at 1664.180 kb on - strand at 1664.208 kb on + strand at 1664.253 kb on + strand at 1664.253 kb on + strand at 1664.254 kb on - strand at 1664.254 kb on - strand at 1664.305 kb on + strand at 1664.332 kb on + strand at 1664.334 kb on + strand at 1664.336 kb on + strand at 1664.364 kb on + strand at 1664.369 kb on + strand, within ompF at 1664.418 kb on + strand, within ompF at 1664.442 kb on + strand, within ompF at 1664.443 kb on - strand, within ompF at 1664.512 kb on - strand, within ompF at 1664.592 kb on + strand, within ompF at 1664.592 kb on + strand, within ompF at 1664.593 kb on - strand, within ompF at 1664.629 kb on - strand, within ompF at 1664.629 kb on - strand, within ompF at 1664.640 kb on + strand, within ompF at 1664.655 kb on + strand, within ompF at 1664.663 kb on + strand, within ompF at 1664.663 kb on + strand, within ompF at 1664.687 kb on + strand, within ompF at 1664.694 kb on + strand, within ompF at 1664.695 kb on - strand, within ompF at 1664.735 kb on + strand, within ompF at 1664.735 kb on + strand, within ompF at 1664.735 kb on + strand, within ompF at 1664.735 kb on + strand, within ompF at 1664.739 kb on + strand, within ompF at 1664.739 kb on + strand, within ompF at 1664.794 kb on - strand, within ompF at 1664.802 kb on - strand, within ompF at 1664.855 kb on + strand, within ompF at 1664.855 kb on + strand, within ompF at 1664.856 kb on - strand, within ompF at 1664.863 kb on - strand, within ompF at 1664.869 kb on - strand, within ompF at 1664.896 kb on - strand, within ompF at 1664.896 kb on - strand, within ompF at 1664.970 kb on - strand, within ompF at 1664.982 kb on + strand, within ompF at 1665.000 kb on + strand, within ompF at 1665.000 kb on + strand, within ompF at 1665.112 kb on - strand, within ompF at 1665.172 kb on + strand, within ompF at 1665.172 kb on + strand, within ompF at 1665.172 kb on + strand, within ompF at 1665.172 kb on + strand, within ompF at 1665.204 kb on + strand, within ompF at 1665.252 kb on + strand at 1665.252 kb on + strand at 1665.252 kb on + strand at 1665.252 kb on + strand at 1665.252 kb on + strand at 1665.261 kb on + strand at 1665.261 kb on + strand at 1665.261 kb on + strand at 1665.261 kb on + strand at 1665.364 kb on + strand at 1665.364 kb on + strand at 1665.460 kb on - strand at 1665.460 kb on - strand at 1665.639 kb on + strand at 1665.858 kb on - strand, within aspC at 1666.098 kb on + strand, within aspC at 1666.261 kb on + strand, within aspC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,663,654 + -0.1 1,663,654 + -0.4 1,663,714 - +1.1 1,663,725 - -0.7 1,663,780 + +0.4 1,663,979 - -2.8 1,663,999 - -1.7 1,664,125 + -1.1 1,664,156 + -0.1 1,664,180 - -0.0 1,664,208 + +2.2 1,664,253 + +0.4 1,664,253 + -1.7 1,664,254 - -0.6 1,664,254 - +0.2 1,664,305 + -0.1 1,664,332 + -0.8 1,664,334 + +1.4 1,664,336 + +0.3 1,664,364 + -0.7 1,664,369 + ompF MCAODC_24770 0.10 -1.0 1,664,418 + ompF MCAODC_24770 0.15 +0.3 1,664,442 + ompF MCAODC_24770 0.17 +4.8 1,664,443 - ompF MCAODC_24770 0.17 +0.9 1,664,512 - ompF MCAODC_24770 0.23 +0.4 1,664,592 + ompF MCAODC_24770 0.31 -1.1 1,664,592 + ompF MCAODC_24770 0.31 +0.0 1,664,593 - ompF MCAODC_24770 0.31 +0.7 1,664,629 - ompF MCAODC_24770 0.34 +1.2 1,664,629 - ompF MCAODC_24770 0.34 +0.1 1,664,640 + ompF MCAODC_24770 0.35 +1.1 1,664,655 + ompF MCAODC_24770 0.36 +1.1 1,664,663 + ompF MCAODC_24770 0.37 -0.4 1,664,663 + ompF MCAODC_24770 0.37 -0.7 1,664,687 + ompF MCAODC_24770 0.39 -1.2 1,664,694 + ompF MCAODC_24770 0.40 +0.8 1,664,695 - ompF MCAODC_24770 0.40 -0.2 1,664,735 + ompF MCAODC_24770 0.44 +0.4 1,664,735 + ompF MCAODC_24770 0.44 +0.5 1,664,735 + ompF MCAODC_24770 0.44 +0.5 1,664,735 + ompF MCAODC_24770 0.44 +1.4 1,664,739 + ompF MCAODC_24770 0.44 +0.2 1,664,739 + ompF MCAODC_24770 0.44 -0.5 1,664,794 - ompF MCAODC_24770 0.49 -0.4 1,664,802 - ompF MCAODC_24770 0.50 +1.2 1,664,855 + ompF MCAODC_24770 0.55 +0.1 1,664,855 + ompF MCAODC_24770 0.55 +0.8 1,664,856 - ompF MCAODC_24770 0.55 +2.1 1,664,863 - ompF MCAODC_24770 0.55 +1.2 1,664,869 - ompF MCAODC_24770 0.56 +0.2 1,664,896 - ompF MCAODC_24770 0.58 +1.1 1,664,896 - ompF MCAODC_24770 0.58 -1.3 1,664,970 - ompF MCAODC_24770 0.65 +1.3 1,664,982 + ompF MCAODC_24770 0.66 +3.8 1,665,000 + ompF MCAODC_24770 0.68 +0.6 1,665,000 + ompF MCAODC_24770 0.68 -2.8 1,665,112 - ompF MCAODC_24770 0.78 -0.7 1,665,172 + ompF MCAODC_24770 0.84 +0.8 1,665,172 + ompF MCAODC_24770 0.84 -0.1 1,665,172 + ompF MCAODC_24770 0.84 -1.1 1,665,172 + ompF MCAODC_24770 0.84 -0.8 1,665,204 + ompF MCAODC_24770 0.87 -0.2 1,665,252 + -0.2 1,665,252 + +0.4 1,665,252 + +0.7 1,665,252 + -2.2 1,665,252 + -1.3 1,665,261 + +0.3 1,665,261 + -2.0 1,665,261 + -2.2 1,665,261 + +0.9 1,665,364 + -0.1 1,665,364 + +0.2 1,665,460 - +0.2 1,665,460 - -0.2 1,665,639 + +0.6 1,665,858 - aspC MCAODC_24775 0.27 +1.8 1,666,098 + aspC MCAODC_24775 0.48 -0.1 1,666,261 + aspC MCAODC_24775 0.61 -1.8
Or see this region's nucleotide sequence