Strain Fitness in Escherichia coli ECRC101 around MCAODC_23915

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteAL and MCAODC_23910 are separated by 97 nucleotidesMCAODC_23910 and MCAODC_23915 overlap by 4 nucleotidesMCAODC_23915 and pgaA are separated by 586 nucleotides MCAODC_23905: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 1,504,210 to 1,505,820 eAL MCAODC_23910: MCAODC_23910 - GGDEF domain-containing protein, at 1,505,918 to 1,506,334 _23910 MCAODC_23915: MCAODC_23915 - Putative diguanylate cyclase, at 1,506,331 to 1,507,275 _23915 MCAODC_23920: pgaA - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 1,507,862 to 1,509,619 pgaA Position (kb) 1506 1507 1508Strain fitness (log2 ratio) -1 0 1 2 3at 1505.616 kb on + strand, within eALat 1505.690 kb on - strandat 1505.772 kb on - strandat 1505.797 kb on - strandat 1505.988 kb on + strand, within MCAODC_23910at 1506.086 kb on - strand, within MCAODC_23910at 1506.231 kb on - strand, within MCAODC_23910at 1506.464 kb on - strand, within MCAODC_23915at 1507.241 kb on - strandat 1507.328 kb on - strandat 1507.419 kb on + strandat 1507.453 kb on - strandat 1507.453 kb on - strandat 1507.607 kb on - strandat 1507.701 kb on + strandat 1507.703 kb on + strandat 1507.844 kb on + strandat 1507.844 kb on + strandat 1507.844 kb on + strandat 1507.856 kb on + strandat 1507.866 kb on - strandat 1507.866 kb on - strandat 1508.008 kb on + strandat 1508.105 kb on - strand, within pgaAat 1508.215 kb on + strand, within pgaA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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1,505,616 + eAL MCAODC_23905 0.87 -0.2
1,505,690 - -1.5
1,505,772 - -0.2
1,505,797 - +1.5
1,505,988 + MCAODC_23910 0.17 -0.8
1,506,086 - MCAODC_23910 0.40 +1.1
1,506,231 - MCAODC_23910 0.75 +0.6
1,506,464 - MCAODC_23915 0.14 +3.0
1,507,241 - -0.1
1,507,328 - -0.0
1,507,419 + +0.8
1,507,453 - +0.2
1,507,453 - -0.1
1,507,607 - -0.4
1,507,701 + -1.2
1,507,703 + -0.2
1,507,844 + -0.9
1,507,844 + -0.6
1,507,844 + +0.2
1,507,856 + +1.8
1,507,866 - +0.5
1,507,866 - -0.1
1,508,008 + +1.0
1,508,105 - pgaA MCAODC_23920 0.14 +0.3
1,508,215 + pgaA MCAODC_23920 0.20 -1.4

Or see this region's nucleotide sequence