Strain Fitness in Escherichia coli ECRC101 around MCAODC_21810

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsirB2 and prmC overlap by 4 nucleotidesprmC and prfA overlap by 1 nucleotidesprfA and hemA are separated by 41 nucleotides MCAODC_21800: sirB2 - invasion regulator SirB2, at 1,142,656 to 1,143,048 sirB2 MCAODC_21805: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 1,143,045 to 1,143,878 prmC MCAODC_21810: prfA - peptide chain release factor 1, at 1,143,878 to 1,144,960 prfA MCAODC_21815: hemA - glutamyl-tRNA reductase, at 1,145,002 to 1,146,258 hemA Position (kb) 1143 1144 1145Strain fitness (log2 ratio) -1 0 1 2 3at 1142.893 kb on + strand, within sirB2at 1142.894 kb on - strand, within sirB2at 1142.966 kb on - strand, within sirB2at 1142.978 kb on - strand, within sirB2at 1142.984 kb on - strand, within sirB2at 1142.984 kb on - strand, within sirB2at 1143.012 kb on + strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.013 kb on - strandat 1143.426 kb on - strand, within prmCat 1143.667 kb on - strand, within prmCat 1143.765 kb on - strand, within prmCat 1143.929 kb on - strandat 1144.998 kb on - strandat 1144.998 kb on - strandat 1145.004 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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1,142,893 + sirB2 MCAODC_21800 0.60 +0.4
1,142,894 - sirB2 MCAODC_21800 0.61 -0.6
1,142,966 - sirB2 MCAODC_21800 0.79 -0.3
1,142,978 - sirB2 MCAODC_21800 0.82 +0.1
1,142,984 - sirB2 MCAODC_21800 0.83 +1.4
1,142,984 - sirB2 MCAODC_21800 0.83 +0.8
1,143,012 + +0.9
1,143,013 - -0.4
1,143,013 - -1.0
1,143,013 - +0.5
1,143,013 - -1.4
1,143,013 - -0.1
1,143,013 - +2.8
1,143,426 - prmC MCAODC_21805 0.46 +1.2
1,143,667 - prmC MCAODC_21805 0.75 +1.4
1,143,765 - prmC MCAODC_21805 0.86 +1.2
1,143,929 - -0.3
1,144,998 - -0.9
1,144,998 - -0.5
1,145,004 - +0.9

Or see this region's nucleotide sequence