Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaA and galF are separated by 318 nucleotides galF and MCAODC_20540 are separated by 242 nucleotides
MCAODC_20530: wcaA - glycosyl transferase, at 914,382 to 915,164
wcaA
MCAODC_20535: galF - UTP--glucose-1-phosphate uridylyltransferase GalF, at 915,483 to 916,376
galF
MCAODC_20540: MCAODC_20540 - N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,o ctacis-undecaprenol 4-epimerase, at 916,619 to 917,614
_20540
Position (kb)
915
916
917 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 914.504 kb on - strand, within wcaA at 914.717 kb on - strand, within wcaA at 915.111 kb on - strand at 915.301 kb on - strand at 915.309 kb on - strand at 915.377 kb on - strand at 915.386 kb on - strand at 915.551 kb on - strand at 915.596 kb on - strand, within galF at 915.696 kb on - strand, within galF at 915.944 kb on - strand, within galF at 916.033 kb on - strand, within galF at 916.079 kb on + strand, within galF at 916.104 kb on - strand, within galF at 916.123 kb on - strand, within galF at 916.123 kb on - strand, within galF at 916.166 kb on - strand, within galF at 916.166 kb on - strand, within galF at 916.262 kb on - strand, within galF at 916.262 kb on - strand, within galF at 916.329 kb on - strand at 916.329 kb on - strand at 916.445 kb on - strand at 916.447 kb on - strand at 916.447 kb on - strand at 916.449 kb on - strand at 916.449 kb on - strand at 916.497 kb on - strand at 916.544 kb on - strand at 916.621 kb on - strand at 916.703 kb on - strand at 916.703 kb on - strand at 916.703 kb on - strand at 916.703 kb on - strand at 916.703 kb on - strand at 916.703 kb on - strand at 916.871 kb on - strand, within MCAODC_20540 at 916.871 kb on - strand, within MCAODC_20540 at 916.871 kb on - strand, within MCAODC_20540 at 916.940 kb on - strand, within MCAODC_20540 at 916.948 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.954 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.032 kb on + strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.034 kb on + strand, within MCAODC_20540 at 917.035 kb on - strand, within MCAODC_20540 at 917.038 kb on + strand, within MCAODC_20540 at 917.039 kb on - strand, within MCAODC_20540 at 917.059 kb on + strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.092 kb on + strand, within MCAODC_20540 at 917.092 kb on + strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.125 kb on + strand, within MCAODC_20540 at 917.143 kb on + strand, within MCAODC_20540 at 917.144 kb on - strand, within MCAODC_20540 at 917.147 kb on + strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.278 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.373 kb on + strand, within MCAODC_20540 at 917.373 kb on + strand, within MCAODC_20540
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 914,504 - wcaA MCAODC_20530 0.16 -1.2 914,717 - wcaA MCAODC_20530 0.43 -1.6 915,111 - +0.6 915,301 - -2.2 915,309 - -0.2 915,377 - +0.2 915,386 - +2.1 915,551 - -1.7 915,596 - galF MCAODC_20535 0.13 -0.7 915,696 - galF MCAODC_20535 0.24 -2.2 915,944 - galF MCAODC_20535 0.52 -0.3 916,033 - galF MCAODC_20535 0.62 +0.4 916,079 + galF MCAODC_20535 0.67 -2.9 916,104 - galF MCAODC_20535 0.69 -2.2 916,123 - galF MCAODC_20535 0.72 -0.4 916,123 - galF MCAODC_20535 0.72 -2.9 916,166 - galF MCAODC_20535 0.76 -0.4 916,166 - galF MCAODC_20535 0.76 -1.6 916,262 - galF MCAODC_20535 0.87 -3.2 916,262 - galF MCAODC_20535 0.87 -0.9 916,329 - -2.8 916,329 - -1.7 916,445 - +1.7 916,447 - -1.0 916,447 - -0.8 916,449 - +0.3 916,449 - +0.3 916,497 - -0.7 916,544 - -2.7 916,621 - +0.7 916,703 - -2.0 916,703 - -0.3 916,703 - -4.7 916,703 - -2.5 916,703 - -3.0 916,703 - -2.3 916,871 - MCAODC_20540 0.25 -3.9 916,871 - MCAODC_20540 0.25 -2.7 916,871 - MCAODC_20540 0.25 +1.1 916,940 - MCAODC_20540 0.32 -2.9 916,948 - MCAODC_20540 0.33 -1.6 916,952 - MCAODC_20540 0.33 -2.0 916,952 - MCAODC_20540 0.33 -5.4 916,952 - MCAODC_20540 0.33 -2.7 916,952 - MCAODC_20540 0.33 -1.9 916,954 - MCAODC_20540 0.34 -2.3 917,020 - MCAODC_20540 0.40 -2.4 917,020 - MCAODC_20540 0.40 -2.6 917,020 - MCAODC_20540 0.40 -1.1 917,020 - MCAODC_20540 0.40 -2.2 917,020 - MCAODC_20540 0.40 -4.3 917,032 + MCAODC_20540 0.41 -2.9 917,033 - MCAODC_20540 0.42 -5.2 917,033 - MCAODC_20540 0.42 -2.7 917,033 - MCAODC_20540 0.42 -2.0 917,033 - MCAODC_20540 0.42 -2.2 917,034 + MCAODC_20540 0.42 -0.5 917,035 - MCAODC_20540 0.42 -3.4 917,038 + MCAODC_20540 0.42 -2.1 917,039 - MCAODC_20540 0.42 -1.7 917,059 + MCAODC_20540 0.44 -0.9 917,060 - MCAODC_20540 0.44 -2.9 917,060 - MCAODC_20540 0.44 -3.7 917,060 - MCAODC_20540 0.44 -1.8 917,092 + MCAODC_20540 0.47 -0.2 917,092 + MCAODC_20540 0.47 -3.0 917,093 - MCAODC_20540 0.48 -3.0 917,093 - MCAODC_20540 0.48 -2.4 917,093 - MCAODC_20540 0.48 -0.4 917,093 - MCAODC_20540 0.48 -1.8 917,125 + MCAODC_20540 0.51 +0.4 917,143 + MCAODC_20540 0.53 +0.0 917,144 - MCAODC_20540 0.53 -2.1 917,147 + MCAODC_20540 0.53 +2.1 917,148 - MCAODC_20540 0.53 -1.9 917,148 - MCAODC_20540 0.53 -2.8 917,148 - MCAODC_20540 0.53 -0.7 917,174 - MCAODC_20540 0.56 -4.1 917,174 - MCAODC_20540 0.56 -1.7 917,174 - MCAODC_20540 0.56 -3.0 917,174 - MCAODC_20540 0.56 -4.2 917,174 - MCAODC_20540 0.56 -2.8 917,174 - MCAODC_20540 0.56 -1.6 917,278 - MCAODC_20540 0.66 -0.1 917,371 - MCAODC_20540 0.76 -1.7 917,371 - MCAODC_20540 0.76 +1.3 917,371 - MCAODC_20540 0.76 -1.3 917,373 + MCAODC_20540 0.76 +0.6 917,373 + MCAODC_20540 0.76 -2.0
Or see this region's nucleotide sequence