Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pptA and yddH are separated by 3 nucleotides yddH and yddH are separated by 3 nucleotides yddH and nhoA are separated by 172 nucleotides nhoA and MCAODC_16495 overlap by 1 nucleotides MCAODC_16495 and yddE are separated by 95 nucleotides
MCAODC_16475: pptA - tautomerase PptA, at 189,861 to 190,088
pptA
MCAODC_16480: yddH - Uncharacterized protein YddH, at 190,092 to 190,535
yddH
MCAODC_16485: yddH - Uncharacterized protein YddH, at 190,539 to 190,661
yddH
MCAODC_16490: nhoA - N-hydroxyarylamine O-acetyltransferase, at 190,834 to 191,568
nhoA
MCAODC_16495: MCAODC_16495 - N-hydroxyarylamine O-acetyltransferase, at 191,568 to 191,678
_16495
MCAODC_16500: yddE - PhzF family isomerase, at 191,774 to 192,667
yddE
Position (kb) 190
191
192 Strain fitness (log2 ratio) -1
0
1
2
3 at 189.879 kb on - strand at 190.022 kb on + strand, within pptA at 190.086 kb on + strand at 190.103 kb on + strand at 190.163 kb on + strand, within yddH at 190.247 kb on - strand, within yddH at 190.357 kb on + strand, within yddH at 190.371 kb on - strand, within yddH at 190.415 kb on + strand, within yddH at 190.415 kb on + strand, within yddH at 190.458 kb on - strand, within yddH at 190.491 kb on - strand at 190.554 kb on - strand, within yddH at 190.554 kb on - strand, within yddH at 190.614 kb on + strand, within yddH at 190.636 kb on - strand, within yddH at 190.641 kb on + strand, within yddH at 190.690 kb on - strand at 190.695 kb on + strand at 190.710 kb on + strand at 190.714 kb on + strand at 190.802 kb on + strand at 190.803 kb on - strand at 190.803 kb on - strand at 191.072 kb on + strand, within nhoA at 191.138 kb on + strand, within nhoA at 191.383 kb on + strand, within nhoA at 191.384 kb on - strand, within nhoA at 191.540 kb on - strand at 191.557 kb on + strand at 191.559 kb on + strand at 191.559 kb on + strand at 191.559 kb on + strand at 191.559 kb on + strand at 191.654 kb on + strand, within MCAODC_16495 at 191.682 kb on - strand at 191.724 kb on - strand at 191.783 kb on - strand at 191.946 kb on - strand, within yddE at 191.958 kb on - strand, within yddE at 192.305 kb on - strand, within yddE at 192.408 kb on - strand, within yddE at 192.422 kb on + strand, within yddE at 192.422 kb on + strand, within yddE at 192.423 kb on - strand, within yddE at 192.423 kb on - strand, within yddE at 192.423 kb on - strand, within yddE at 192.423 kb on - strand, within yddE at 192.426 kb on + strand, within yddE at 192.438 kb on - strand, within yddE at 192.457 kb on + strand, within yddE at 192.457 kb on + strand, within yddE at 192.542 kb on + strand, within yddE
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 189,879 - +0.4 190,022 + pptA MCAODC_16475 0.71 +1.3 190,086 + +0.6 190,103 + +0.4 190,163 + yddH MCAODC_16480 0.16 +1.5 190,247 - yddH MCAODC_16480 0.35 +2.7 190,357 + yddH MCAODC_16480 0.60 -1.0 190,371 - yddH MCAODC_16480 0.63 -1.2 190,415 + yddH MCAODC_16480 0.73 +1.5 190,415 + yddH MCAODC_16480 0.73 +1.9 190,458 - yddH MCAODC_16480 0.82 -0.1 190,491 - -0.8 190,554 - yddH MCAODC_16485 0.12 +1.7 190,554 - yddH MCAODC_16485 0.12 -0.1 190,614 + yddH MCAODC_16485 0.61 +0.7 190,636 - yddH MCAODC_16485 0.79 +1.2 190,641 + yddH MCAODC_16485 0.83 -0.6 190,690 - -0.1 190,695 + -0.8 190,710 + +0.1 190,714 + +0.8 190,802 + -0.4 190,803 - -0.3 190,803 - +1.1 191,072 + nhoA MCAODC_16490 0.32 -0.5 191,138 + nhoA MCAODC_16490 0.41 +0.9 191,383 + nhoA MCAODC_16490 0.75 -1.5 191,384 - nhoA MCAODC_16490 0.75 +3.7 191,540 - +1.1 191,557 + +1.9 191,559 + +2.3 191,559 + +0.7 191,559 + +1.7 191,559 + +0.2 191,654 + MCAODC_16495 0.77 +0.9 191,682 - -1.3 191,724 - -1.2 191,783 - -0.0 191,946 - yddE MCAODC_16500 0.19 -0.0 191,958 - yddE MCAODC_16500 0.21 -0.6 192,305 - yddE MCAODC_16500 0.59 +0.5 192,408 - yddE MCAODC_16500 0.71 +0.8 192,422 + yddE MCAODC_16500 0.72 -0.6 192,422 + yddE MCAODC_16500 0.72 +1.4 192,423 - yddE MCAODC_16500 0.73 +2.3 192,423 - yddE MCAODC_16500 0.73 +0.7 192,423 - yddE MCAODC_16500 0.73 +0.4 192,423 - yddE MCAODC_16500 0.73 +1.1 192,426 + yddE MCAODC_16500 0.73 +1.2 192,438 - yddE MCAODC_16500 0.74 -0.2 192,457 + yddE MCAODC_16500 0.76 -0.9 192,457 + yddE MCAODC_16500 0.76 -0.2 192,542 + yddE MCAODC_16500 0.86 -0.4
Or see this region's nucleotide sequence