Strain Fitness in Escherichia coli ECRC101 around MCAODC_15605

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpCF and trpD are separated by 3 nucleotidestrpD and trpE overlap by 1 nucleotides MCAODC_15600: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 9,434 to 10,792 trpCF MCAODC_15605: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 10,796 to 12,391 trpD MCAODC_15610: trpE - anthranilate synthase component I, at 12,391 to 13,953 trpE Position (kb) 10 11 12 13Strain fitness (log2 ratio) -2 -1 0 1 2 3at 9.874 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.134 kb on + strand, within trpCFat 10.135 kb on - strand, within trpCFat 10.139 kb on - strand, within trpCFat 10.139 kb on - strand, within trpCFat 10.202 kb on - strand, within trpCFat 10.388 kb on + strand, within trpCFat 10.389 kb on - strand, within trpCFat 10.422 kb on + strand, within trpCFat 10.423 kb on - strand, within trpCFat 10.423 kb on - strand, within trpCFat 10.454 kb on - strand, within trpCFat 10.454 kb on - strand, within trpCFat 10.555 kb on - strand, within trpCFat 10.643 kb on - strand, within trpCFat 10.831 kb on - strandat 11.064 kb on + strand, within trpDat 11.065 kb on - strand, within trpDat 11.072 kb on - strand, within trpDat 11.114 kb on + strand, within trpDat 11.164 kb on - strand, within trpDat 11.213 kb on - strand, within trpDat 11.213 kb on - strand, within trpDat 11.226 kb on - strand, within trpDat 11.357 kb on + strand, within trpDat 11.357 kb on + strand, within trpDat 11.357 kb on + strand, within trpDat 11.358 kb on - strand, within trpDat 11.358 kb on - strand, within trpDat 11.358 kb on - strand, within trpDat 11.363 kb on + strand, within trpDat 11.363 kb on + strand, within trpDat 11.390 kb on - strand, within trpDat 11.390 kb on - strand, within trpDat 11.410 kb on + strand, within trpDat 11.715 kb on - strand, within trpDat 11.978 kb on - strand, within trpDat 12.102 kb on + strand, within trpDat 12.103 kb on - strand, within trpDat 12.154 kb on + strand, within trpDat 12.154 kb on + strand, within trpDat 12.193 kb on + strand, within trpDat 12.193 kb on + strand, within trpDat 12.194 kb on - strand, within trpDat 12.194 kb on - strand, within trpDat 12.194 kb on - strand, within trpDat 12.273 kb on - strandat 12.313 kb on + strandat 12.349 kb on - strandat 12.353 kb on - strandat 12.365 kb on - strandat 12.392 kb on + strandat 12.392 kb on + strandat 12.392 kb on + strandat 12.426 kb on + strandat 12.590 kb on + strand, within trpEat 12.591 kb on - strand, within trpEat 12.595 kb on + strand, within trpEat 12.596 kb on - strand, within trpEat 12.658 kb on + strand, within trpEat 12.770 kb on + strand, within trpEat 12.770 kb on + strand, within trpEat 12.771 kb on - strand, within trpEat 12.886 kb on - strand, within trpEat 12.984 kb on - strand, within trpEat 13.013 kb on + strand, within trpEat 13.014 kb on - strand, within trpEat 13.079 kb on + strand, within trpEat 13.080 kb on - strand, within trpEat 13.109 kb on + strand, within trpEat 13.110 kb on - strand, within trpEat 13.113 kb on - strand, within trpE

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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9,874 - trpCF MCAODC_15600 0.32 +0.2
10,017 - trpCF MCAODC_15600 0.43 -0.3
10,017 - trpCF MCAODC_15600 0.43 +1.6
10,017 - trpCF MCAODC_15600 0.43 -0.6
10,017 - trpCF MCAODC_15600 0.43 -1.3
10,134 + trpCF MCAODC_15600 0.52 -0.3
10,135 - trpCF MCAODC_15600 0.52 +1.7
10,139 - trpCF MCAODC_15600 0.52 -0.9
10,139 - trpCF MCAODC_15600 0.52 -1.3
10,202 - trpCF MCAODC_15600 0.57 +1.7
10,388 + trpCF MCAODC_15600 0.70 +2.7
10,389 - trpCF MCAODC_15600 0.70 +0.4
10,422 + trpCF MCAODC_15600 0.73 -0.4
10,423 - trpCF MCAODC_15600 0.73 -0.7
10,423 - trpCF MCAODC_15600 0.73 +0.4
10,454 - trpCF MCAODC_15600 0.75 +0.1
10,454 - trpCF MCAODC_15600 0.75 +0.3
10,555 - trpCF MCAODC_15600 0.82 +1.3
10,643 - trpCF MCAODC_15600 0.89 -1.9
10,831 - +1.0
11,064 + trpD MCAODC_15605 0.17 +0.7
11,065 - trpD MCAODC_15605 0.17 +0.3
11,072 - trpD MCAODC_15605 0.17 +0.4
11,114 + trpD MCAODC_15605 0.20 -0.4
11,164 - trpD MCAODC_15605 0.23 -0.3
11,213 - trpD MCAODC_15605 0.26 -1.4
11,213 - trpD MCAODC_15605 0.26 +1.0
11,226 - trpD MCAODC_15605 0.27 +0.3
11,357 + trpD MCAODC_15605 0.35 -0.4
11,357 + trpD MCAODC_15605 0.35 +1.5
11,357 + trpD MCAODC_15605 0.35 +1.2
11,358 - trpD MCAODC_15605 0.35 +0.7
11,358 - trpD MCAODC_15605 0.35 -1.7
11,358 - trpD MCAODC_15605 0.35 -0.7
11,363 + trpD MCAODC_15605 0.36 -0.2
11,363 + trpD MCAODC_15605 0.36 -0.6
11,390 - trpD MCAODC_15605 0.37 -0.1
11,390 - trpD MCAODC_15605 0.37 -1.2
11,410 + trpD MCAODC_15605 0.38 +0.8
11,715 - trpD MCAODC_15605 0.58 -0.8
11,978 - trpD MCAODC_15605 0.74 +0.5
12,102 + trpD MCAODC_15605 0.82 +3.4
12,103 - trpD MCAODC_15605 0.82 +0.5
12,154 + trpD MCAODC_15605 0.85 -1.1
12,154 + trpD MCAODC_15605 0.85 -1.5
12,193 + trpD MCAODC_15605 0.88 -0.5
12,193 + trpD MCAODC_15605 0.88 -0.6
12,194 - trpD MCAODC_15605 0.88 +1.0
12,194 - trpD MCAODC_15605 0.88 +0.7
12,194 - trpD MCAODC_15605 0.88 -0.5
12,273 - +1.3
12,313 + -0.0
12,349 - +0.1
12,353 - +0.7
12,365 - +1.5
12,392 + +1.4
12,392 + -0.1
12,392 + +0.1
12,426 + +0.5
12,590 + trpE MCAODC_15610 0.13 +1.1
12,591 - trpE MCAODC_15610 0.13 +0.2
12,595 + trpE MCAODC_15610 0.13 +1.5
12,596 - trpE MCAODC_15610 0.13 +0.3
12,658 + trpE MCAODC_15610 0.17 +0.2
12,770 + trpE MCAODC_15610 0.24 +0.1
12,770 + trpE MCAODC_15610 0.24 -1.3
12,771 - trpE MCAODC_15610 0.24 +0.7
12,886 - trpE MCAODC_15610 0.32 -2.4
12,984 - trpE MCAODC_15610 0.38 -0.5
13,013 + trpE MCAODC_15610 0.40 -0.1
13,014 - trpE MCAODC_15610 0.40 +2.1
13,079 + trpE MCAODC_15610 0.44 -0.9
13,080 - trpE MCAODC_15610 0.44 +1.9
13,109 + trpE MCAODC_15610 0.46 -0.0
13,110 - trpE MCAODC_15610 0.46 +0.2
13,113 - trpE MCAODC_15610 0.46 +1.5

Or see this region's nucleotide sequence