Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psuK and psuG overlap by 13 nucleotides psuG and psuT are separated by 93 nucleotides
MCAODC_14500: psuK - pseudouridine kinase, at 2,853,496 to 2,854,437
psuK
MCAODC_14505: psuG - pseudouridine-5'-phosphate glycosidase, at 2,854,425 to 2,855,363
psuG
MCAODC_14510: psuT - Putative pseudouridine transporter, at 2,855,457 to 2,856,707
psuT
Position (kb)
2854
2855
2856 Strain fitness (log2 ratio)
-2
-1
0
1 at 2853.425 kb on + strand at 2853.445 kb on + strand at 2853.445 kb on + strand at 2853.446 kb on - strand at 2853.446 kb on - strand at 2853.924 kb on + strand, within psuK at 2854.158 kb on - strand, within psuK at 2854.183 kb on + strand, within psuK at 2854.183 kb on + strand, within psuK at 2854.239 kb on + strand, within psuK at 2854.239 kb on + strand, within psuK at 2854.242 kb on + strand, within psuK at 2854.242 kb on + strand, within psuK at 2854.804 kb on + strand, within psuG at 2855.166 kb on - strand, within psuG at 2855.166 kb on - strand, within psuG at 2855.192 kb on - strand, within psuG at 2855.216 kb on + strand, within psuG at 2855.457 kb on - strand at 2855.464 kb on + strand at 2855.464 kb on + strand at 2855.503 kb on + strand at 2855.525 kb on + strand at 2855.598 kb on + strand, within psuT at 2855.599 kb on - strand, within psuT at 2855.623 kb on + strand, within psuT at 2855.623 kb on + strand, within psuT at 2855.624 kb on - strand, within psuT at 2855.624 kb on - strand, within psuT at 2855.624 kb on - strand, within psuT at 2855.638 kb on + strand, within psuT at 2855.661 kb on + strand, within psuT at 2855.758 kb on + strand, within psuT at 2855.803 kb on - strand, within psuT at 2855.805 kb on + strand, within psuT at 2855.939 kb on + strand, within psuT at 2855.979 kb on - strand, within psuT at 2856.031 kb on - strand, within psuT at 2856.046 kb on + strand, within psuT at 2856.057 kb on + strand, within psuT at 2856.058 kb on - strand, within psuT at 2856.111 kb on - strand, within psuT at 2856.117 kb on - strand, within psuT at 2856.273 kb on - strand, within psuT at 2856.293 kb on + strand, within psuT
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 2,853,425 + -2.1 2,853,445 + -2.2 2,853,445 + -0.3 2,853,446 - +0.9 2,853,446 - -0.3 2,853,924 + psuK MCAODC_14500 0.45 +0.1 2,854,158 - psuK MCAODC_14500 0.70 -0.9 2,854,183 + psuK MCAODC_14500 0.73 -0.6 2,854,183 + psuK MCAODC_14500 0.73 -0.4 2,854,239 + psuK MCAODC_14500 0.79 -0.5 2,854,239 + psuK MCAODC_14500 0.79 +0.1 2,854,242 + psuK MCAODC_14500 0.79 -0.7 2,854,242 + psuK MCAODC_14500 0.79 -1.3 2,854,804 + psuG MCAODC_14505 0.40 -0.2 2,855,166 - psuG MCAODC_14505 0.79 -0.9 2,855,166 - psuG MCAODC_14505 0.79 -1.0 2,855,192 - psuG MCAODC_14505 0.82 +1.3 2,855,216 + psuG MCAODC_14505 0.84 +0.5 2,855,457 - -0.4 2,855,464 + +0.9 2,855,464 + -0.9 2,855,503 + -0.7 2,855,525 + -0.5 2,855,598 + psuT MCAODC_14510 0.11 -0.8 2,855,599 - psuT MCAODC_14510 0.11 +0.6 2,855,623 + psuT MCAODC_14510 0.13 +0.7 2,855,623 + psuT MCAODC_14510 0.13 -0.7 2,855,624 - psuT MCAODC_14510 0.13 +0.7 2,855,624 - psuT MCAODC_14510 0.13 -0.3 2,855,624 - psuT MCAODC_14510 0.13 +1.2 2,855,638 + psuT MCAODC_14510 0.14 -2.1 2,855,661 + psuT MCAODC_14510 0.16 -0.5 2,855,758 + psuT MCAODC_14510 0.24 +1.0 2,855,803 - psuT MCAODC_14510 0.28 +1.1 2,855,805 + psuT MCAODC_14510 0.28 +0.4 2,855,939 + psuT MCAODC_14510 0.39 -0.6 2,855,979 - psuT MCAODC_14510 0.42 -0.1 2,856,031 - psuT MCAODC_14510 0.46 +0.2 2,856,046 + psuT MCAODC_14510 0.47 +1.1 2,856,057 + psuT MCAODC_14510 0.48 +1.5 2,856,058 - psuT MCAODC_14510 0.48 -0.6 2,856,111 - psuT MCAODC_14510 0.52 +0.0 2,856,117 - psuT MCAODC_14510 0.53 -0.7 2,856,273 - psuT MCAODC_14510 0.65 +0.6 2,856,293 + psuT MCAODC_14510 0.67 -1.1
Or see this region's nucleotide sequence