Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt scpC and scpB are separated by 23 nucleotides scpB and meaB are separated by 10 nucleotides
MCAODC_09950: scpC - propionyl-CoA:succinate CoA transferase, at 1,981,041 to 1,982,519
scpC
MCAODC_09955: scpB - methylmalonyl-CoA decarboxylase, at 1,982,543 to 1,983,328
scpB
MCAODC_09960: meaB - methylmalonyl Co-A mutase-associated GTPase MeaB, at 1,983,339 to 1,984,334
meaB
MCAODC_09965: scpA - methylmalonyl-CoA mutase, at 1,984,327 to 1,986,471
scpA
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1981.547 kb on - strand, within scpC at 1981.547 kb on - strand, within scpC at 1981.636 kb on - strand, within scpC at 1981.657 kb on - strand, within scpC at 1981.670 kb on - strand, within scpC at 1981.676 kb on + strand, within scpC at 1981.725 kb on - strand, within scpC at 1981.731 kb on - strand, within scpC at 1981.857 kb on - strand, within scpC at 1981.860 kb on - strand, within scpC at 1981.922 kb on - strand, within scpC at 1981.964 kb on + strand, within scpC at 1982.029 kb on + strand, within scpC at 1982.173 kb on + strand, within scpC at 1982.305 kb on - strand, within scpC at 1982.384 kb on + strand at 1982.384 kb on + strand at 1982.435 kb on + strand at 1982.444 kb on + strand at 1982.462 kb on + strand at 1982.560 kb on + strand at 1982.560 kb on + strand at 1982.566 kb on + strand at 1982.641 kb on + strand, within scpB at 1982.642 kb on - strand, within scpB at 1982.753 kb on + strand, within scpB at 1982.908 kb on + strand, within scpB at 1982.909 kb on - strand, within scpB at 1982.953 kb on + strand, within scpB at 1983.014 kb on - strand, within scpB at 1983.014 kb on - strand, within scpB at 1983.032 kb on - strand, within scpB at 1983.087 kb on + strand, within scpB at 1983.087 kb on + strand, within scpB at 1983.088 kb on - strand, within scpB at 1983.178 kb on + strand, within scpB at 1983.221 kb on - strand, within scpB at 1983.221 kb on - strand, within scpB at 1983.221 kb on - strand, within scpB at 1983.232 kb on + strand, within scpB at 1983.241 kb on + strand, within scpB at 1983.245 kb on + strand, within scpB at 1983.253 kb on + strand at 1983.254 kb on - strand at 1983.267 kb on + strand at 1983.268 kb on - strand at 1983.268 kb on - strand at 1983.439 kb on + strand, within meaB at 1983.440 kb on - strand, within meaB at 1983.478 kb on - strand, within meaB at 1983.498 kb on - strand, within meaB at 1983.537 kb on + strand, within meaB at 1983.537 kb on + strand, within meaB at 1983.621 kb on - strand, within meaB at 1983.652 kb on + strand, within meaB at 1983.652 kb on + strand, within meaB at 1983.656 kb on + strand, within meaB at 1983.657 kb on - strand, within meaB at 1983.657 kb on - strand, within meaB at 1983.657 kb on - strand, within meaB at 1983.657 kb on - strand, within meaB at 1983.668 kb on + strand, within meaB at 1983.668 kb on + strand, within meaB at 1983.669 kb on - strand, within meaB at 1983.685 kb on + strand, within meaB at 1983.685 kb on + strand, within meaB at 1983.686 kb on - strand, within meaB at 1983.686 kb on - strand, within meaB at 1983.686 kb on - strand, within meaB at 1983.689 kb on + strand, within meaB at 1983.689 kb on + strand, within meaB at 1983.690 kb on - strand, within meaB at 1983.690 kb on - strand, within meaB at 1983.690 kb on - strand, within meaB at 1983.690 kb on - strand, within meaB at 1983.690 kb on - strand, within meaB at 1983.713 kb on + strand, within meaB at 1983.714 kb on - strand, within meaB at 1983.822 kb on - strand, within meaB at 1983.822 kb on - strand, within meaB at 1983.888 kb on + strand, within meaB at 1983.888 kb on + strand, within meaB at 1983.888 kb on + strand, within meaB at 1983.888 kb on + strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.889 kb on - strand, within meaB at 1983.913 kb on - strand, within meaB at 1983.921 kb on + strand, within meaB at 1983.922 kb on - strand, within meaB at 1983.922 kb on - strand, within meaB at 1984.131 kb on - strand, within meaB at 1984.186 kb on + strand, within meaB at 1984.194 kb on - strand, within meaB at 1984.215 kb on - strand, within meaB at 1984.215 kb on - strand, within meaB at 1984.215 kb on - strand, within meaB at 1984.215 kb on - strand, within meaB at 1984.215 kb on - strand, within meaB at 1984.293 kb on + strand at 1984.304 kb on + strand at 1984.305 kb on - strand at 1984.305 kb on - strand at 1984.328 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,981,547 - scpC MCAODC_09950 0.34 +1.3 1,981,547 - scpC MCAODC_09950 0.34 -1.0 1,981,636 - scpC MCAODC_09950 0.40 +1.1 1,981,657 - scpC MCAODC_09950 0.42 -1.8 1,981,670 - scpC MCAODC_09950 0.43 -0.6 1,981,676 + scpC MCAODC_09950 0.43 -0.4 1,981,725 - scpC MCAODC_09950 0.46 +0.5 1,981,731 - scpC MCAODC_09950 0.47 -0.8 1,981,857 - scpC MCAODC_09950 0.55 -0.9 1,981,860 - scpC MCAODC_09950 0.55 +0.5 1,981,922 - scpC MCAODC_09950 0.60 -0.3 1,981,964 + scpC MCAODC_09950 0.62 +0.4 1,982,029 + scpC MCAODC_09950 0.67 +0.9 1,982,173 + scpC MCAODC_09950 0.77 +0.1 1,982,305 - scpC MCAODC_09950 0.85 +0.8 1,982,384 + +0.1 1,982,384 + +0.8 1,982,435 + +0.5 1,982,444 + -0.1 1,982,462 + +1.2 1,982,560 + -0.7 1,982,560 + +1.4 1,982,566 + +0.9 1,982,641 + scpB MCAODC_09955 0.12 +1.1 1,982,642 - scpB MCAODC_09955 0.13 -0.3 1,982,753 + scpB MCAODC_09955 0.27 -1.2 1,982,908 + scpB MCAODC_09955 0.46 +0.2 1,982,909 - scpB MCAODC_09955 0.47 -0.4 1,982,953 + scpB MCAODC_09955 0.52 -0.0 1,983,014 - scpB MCAODC_09955 0.60 -0.2 1,983,014 - scpB MCAODC_09955 0.60 -1.6 1,983,032 - scpB MCAODC_09955 0.62 -0.6 1,983,087 + scpB MCAODC_09955 0.69 +0.7 1,983,087 + scpB MCAODC_09955 0.69 -0.2 1,983,088 - scpB MCAODC_09955 0.69 -2.6 1,983,178 + scpB MCAODC_09955 0.81 -0.6 1,983,221 - scpB MCAODC_09955 0.86 +0.5 1,983,221 - scpB MCAODC_09955 0.86 -1.7 1,983,221 - scpB MCAODC_09955 0.86 +0.3 1,983,232 + scpB MCAODC_09955 0.88 -1.9 1,983,241 + scpB MCAODC_09955 0.89 +0.6 1,983,245 + scpB MCAODC_09955 0.89 -1.7 1,983,253 + -0.2 1,983,254 - -1.2 1,983,267 + -0.1 1,983,268 - -2.6 1,983,268 - -0.8 1,983,439 + meaB MCAODC_09960 0.10 +1.1 1,983,440 - meaB MCAODC_09960 0.10 +1.6 1,983,478 - meaB MCAODC_09960 0.14 -2.0 1,983,498 - meaB MCAODC_09960 0.16 -0.1 1,983,537 + meaB MCAODC_09960 0.20 -0.2 1,983,537 + meaB MCAODC_09960 0.20 +0.4 1,983,621 - meaB MCAODC_09960 0.28 -0.5 1,983,652 + meaB MCAODC_09960 0.31 +0.6 1,983,652 + meaB MCAODC_09960 0.31 -1.1 1,983,656 + meaB MCAODC_09960 0.32 +0.1 1,983,657 - meaB MCAODC_09960 0.32 -0.9 1,983,657 - meaB MCAODC_09960 0.32 +1.7 1,983,657 - meaB MCAODC_09960 0.32 +0.2 1,983,657 - meaB MCAODC_09960 0.32 -0.2 1,983,668 + meaB MCAODC_09960 0.33 +0.9 1,983,668 + meaB MCAODC_09960 0.33 -1.3 1,983,669 - meaB MCAODC_09960 0.33 +1.4 1,983,685 + meaB MCAODC_09960 0.35 -1.1 1,983,685 + meaB MCAODC_09960 0.35 +0.1 1,983,686 - meaB MCAODC_09960 0.35 +0.4 1,983,686 - meaB MCAODC_09960 0.35 -0.9 1,983,686 - meaB MCAODC_09960 0.35 +0.3 1,983,689 + meaB MCAODC_09960 0.35 +0.2 1,983,689 + meaB MCAODC_09960 0.35 +0.5 1,983,690 - meaB MCAODC_09960 0.35 +0.3 1,983,690 - meaB MCAODC_09960 0.35 +2.1 1,983,690 - meaB MCAODC_09960 0.35 +0.6 1,983,690 - meaB MCAODC_09960 0.35 -0.9 1,983,690 - meaB MCAODC_09960 0.35 -0.5 1,983,713 + meaB MCAODC_09960 0.38 -0.6 1,983,714 - meaB MCAODC_09960 0.38 -0.6 1,983,822 - meaB MCAODC_09960 0.48 +0.0 1,983,822 - meaB MCAODC_09960 0.48 -0.4 1,983,888 + meaB MCAODC_09960 0.55 -1.2 1,983,888 + meaB MCAODC_09960 0.55 +1.4 1,983,888 + meaB MCAODC_09960 0.55 +0.9 1,983,888 + meaB MCAODC_09960 0.55 -1.3 1,983,889 - meaB MCAODC_09960 0.55 +1.2 1,983,889 - meaB MCAODC_09960 0.55 -1.6 1,983,889 - meaB MCAODC_09960 0.55 -0.6 1,983,889 - meaB MCAODC_09960 0.55 +1.2 1,983,889 - meaB MCAODC_09960 0.55 -0.3 1,983,889 - meaB MCAODC_09960 0.55 +1.0 1,983,913 - meaB MCAODC_09960 0.58 +0.7 1,983,921 + meaB MCAODC_09960 0.58 +0.5 1,983,922 - meaB MCAODC_09960 0.59 -0.3 1,983,922 - meaB MCAODC_09960 0.59 -1.6 1,984,131 - meaB MCAODC_09960 0.80 -0.1 1,984,186 + meaB MCAODC_09960 0.85 +1.6 1,984,194 - meaB MCAODC_09960 0.86 -0.8 1,984,215 - meaB MCAODC_09960 0.88 -1.1 1,984,215 - meaB MCAODC_09960 0.88 -0.3 1,984,215 - meaB MCAODC_09960 0.88 -1.3 1,984,215 - meaB MCAODC_09960 0.88 +0.5 1,984,215 - meaB MCAODC_09960 0.88 +0.7 1,984,293 + -0.2 1,984,304 + +0.3 1,984,305 - +0.8 1,984,305 - -0.1 1,984,328 + +0.7
Or see this region's nucleotide sequence