Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murA and sfsB are separated by 47 nucleotides sfsB and ispB are separated by 228 nucleotides ispB and rplU are separated by 258 nucleotides rplU and rpmA are separated by 20 nucleotides rpmA and yhbE are separated by 126 nucleotides
MCAODC_08525: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,707,348 to 1,708,607
murA
MCAODC_08530: sfsB - DNA-binding transcriptional regulator SfsB, at 1,708,655 to 1,708,933
sfsB
MCAODC_08535: ispB - octaprenyl diphosphate synthase, at 1,709,162 to 1,710,133
ispB
MCAODC_08540: rplU - 50S ribosomal protein L21, at 1,710,392 to 1,710,703
rplU
MCAODC_08545: rpmA - 50S ribosomal protein L27, at 1,710,724 to 1,710,981
rpmA
MCAODC_08550: yhbE - Uncharacterized inner membrane transporter YhbE, at 1,711,108 to 1,712,073
yhbE
Position (kb)
1709
1710
1711 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1708.606 kb on - strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.674 kb on + strand at 1708.675 kb on - strand at 1708.679 kb on - strand at 1708.679 kb on - strand at 1708.777 kb on + strand, within sfsB at 1708.777 kb on + strand, within sfsB at 1708.925 kb on - strand at 1708.925 kb on - strand at 1708.949 kb on - strand at 1708.954 kb on - strand at 1708.994 kb on + strand at 1708.995 kb on - strand at 1708.996 kb on + strand at 1709.032 kb on - strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.065 kb on + strand at 1709.120 kb on - strand at 1710.167 kb on - strand at 1710.167 kb on - strand at 1710.187 kb on - strand at 1710.194 kb on - strand at 1710.194 kb on - strand at 1710.194 kb on - strand at 1710.194 kb on - strand at 1710.194 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.206 kb on - strand at 1710.208 kb on - strand at 1710.208 kb on - strand at 1710.238 kb on + strand at 1710.239 kb on - strand at 1710.239 kb on - strand at 1710.251 kb on - strand at 1710.277 kb on - strand at 1710.285 kb on + strand at 1710.285 kb on + strand at 1710.285 kb on + strand at 1710.286 kb on - strand at 1710.286 kb on - strand at 1710.286 kb on - strand at 1711.036 kb on + strand at 1711.036 kb on + strand at 1711.036 kb on + strand at 1711.075 kb on + strand at 1711.102 kb on + strand at 1711.102 kb on + strand at 1711.103 kb on - strand at 1711.103 kb on - strand at 1711.103 kb on - strand at 1711.107 kb on + strand at 1711.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,708,606 - +0.9 1,708,674 + -0.2 1,708,674 + +0.3 1,708,674 + -1.0 1,708,674 + -0.0 1,708,675 - +1.2 1,708,679 - -0.2 1,708,679 - -0.7 1,708,777 + sfsB MCAODC_08530 0.44 -0.7 1,708,777 + sfsB MCAODC_08530 0.44 +0.4 1,708,925 - +1.0 1,708,925 - -0.4 1,708,949 - -1.4 1,708,954 - -1.0 1,708,994 + -0.2 1,708,995 - +0.5 1,708,996 + +0.2 1,709,032 - +1.5 1,709,065 + +0.6 1,709,065 + +0.6 1,709,065 + +0.9 1,709,065 + -0.1 1,709,120 - -0.9 1,710,167 - +2.0 1,710,167 - +1.0 1,710,187 - +1.1 1,710,194 - -0.1 1,710,194 - +1.0 1,710,194 - +1.7 1,710,194 - +1.4 1,710,194 - +0.2 1,710,206 - -0.6 1,710,206 - +1.6 1,710,206 - -0.6 1,710,206 - +0.5 1,710,206 - -0.3 1,710,208 - +1.4 1,710,208 - -0.1 1,710,238 + -1.1 1,710,239 - -2.4 1,710,239 - -2.1 1,710,251 - +1.7 1,710,277 - -0.1 1,710,285 + -0.8 1,710,285 + +1.5 1,710,285 + -0.8 1,710,286 - +0.1 1,710,286 - -0.4 1,710,286 - -0.9 1,711,036 + -0.2 1,711,036 + -1.1 1,711,036 + -1.8 1,711,075 + -1.3 1,711,102 + -0.3 1,711,102 + -1.1 1,711,103 - +0.8 1,711,103 - +1.5 1,711,103 - +1.8 1,711,107 + -1.3 1,711,107 + +0.1
Or see this region's nucleotide sequence