Strain Fitness in Escherichia coli ECRC101 around MCAODC_06540

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntghrB and yiaD are separated by 103 nucleotidesyiaD and bisC are separated by 152 nucleotides MCAODC_06535: ghrB - glyoxylate/hydroxypyruvate reductase GhrB, at 1,320,312 to 1,321,286 ghrB MCAODC_06540: yiaD - OmpA family lipoprotein, at 1,321,390 to 1,322,049 yiaD MCAODC_06545: bisC - biotin sulfoxide reductase, at 1,322,202 to 1,324,535 bisC Position (kb) 1321 1322 1323Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1320.432 kb on - strand, within ghrBat 1320.439 kb on - strand, within ghrBat 1320.658 kb on - strand, within ghrBat 1320.693 kb on + strand, within ghrBat 1320.693 kb on + strand, within ghrBat 1320.694 kb on - strand, within ghrBat 1320.694 kb on - strand, within ghrBat 1320.694 kb on - strand, within ghrBat 1320.702 kb on - strand, within ghrBat 1320.746 kb on + strand, within ghrBat 1320.764 kb on + strand, within ghrBat 1320.766 kb on + strand, within ghrBat 1320.766 kb on + strand, within ghrBat 1320.767 kb on - strand, within ghrBat 1320.885 kb on + strand, within ghrBat 1320.885 kb on + strand, within ghrBat 1320.885 kb on + strand, within ghrBat 1320.913 kb on - strand, within ghrBat 1320.924 kb on + strand, within ghrBat 1320.991 kb on - strand, within ghrBat 1321.057 kb on + strand, within ghrBat 1321.058 kb on - strand, within ghrBat 1321.242 kb on + strandat 1321.242 kb on + strandat 1321.242 kb on + strandat 1321.243 kb on - strandat 1321.243 kb on - strandat 1321.254 kb on + strandat 1321.264 kb on + strandat 1321.306 kb on + strandat 1321.307 kb on - strandat 1321.320 kb on + strandat 1321.330 kb on + strandat 1321.331 kb on - strandat 1321.331 kb on - strandat 1321.331 kb on - strandat 1321.391 kb on + strandat 1321.407 kb on + strandat 1321.408 kb on - strandat 1321.597 kb on - strand, within yiaDat 1321.598 kb on + strand, within yiaDat 1321.599 kb on - strand, within yiaDat 1321.599 kb on - strand, within yiaDat 1321.599 kb on - strand, within yiaDat 1321.599 kb on - strand, within yiaDat 1321.722 kb on + strand, within yiaDat 1321.732 kb on - strand, within yiaDat 1321.753 kb on + strand, within yiaDat 1321.758 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.811 kb on + strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.812 kb on - strand, within yiaDat 1321.815 kb on - strand, within yiaDat 1321.815 kb on - strand, within yiaDat 1321.974 kb on - strand, within yiaDat 1321.974 kb on - strand, within yiaDat 1321.974 kb on - strand, within yiaDat 1321.980 kb on + strand, within yiaDat 1321.980 kb on + strand, within yiaDat 1321.981 kb on - strand, within yiaDat 1321.996 kb on + strandat 1322.029 kb on - strandat 1322.060 kb on + strandat 1322.060 kb on + strandat 1322.060 kb on + strandat 1322.061 kb on - strandat 1322.061 kb on - strandat 1322.063 kb on + strandat 1322.104 kb on + strandat 1322.104 kb on + strandat 1322.126 kb on + strandat 1322.126 kb on + strandat 1322.127 kb on - strandat 1322.127 kb on - strandat 1322.156 kb on + strandat 1322.156 kb on + strandat 1322.160 kb on - strandat 1322.164 kb on - strandat 1322.164 kb on - strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.174 kb on + strandat 1322.175 kb on - strandat 1322.175 kb on - strandat 1322.175 kb on - strandat 1322.180 kb on + strandat 1322.180 kb on + strandat 1322.180 kb on + strandat 1322.181 kb on - strandat 1322.289 kb on - strandat 1322.364 kb on - strandat 1322.364 kb on - strandat 1322.481 kb on - strand, within bisCat 1322.481 kb on - strand, within bisCat 1322.495 kb on + strand, within bisCat 1322.496 kb on - strand, within bisCat 1322.514 kb on + strand, within bisCat 1322.514 kb on + strand, within bisCat 1322.515 kb on - strand, within bisCat 1322.515 kb on - strand, within bisCat 1322.544 kb on + strand, within bisCat 1322.574 kb on - strand, within bisCat 1322.574 kb on - strand, within bisCat 1322.588 kb on - strand, within bisCat 1322.588 kb on - strand, within bisCat 1322.588 kb on - strand, within bisCat 1322.590 kb on + strand, within bisCat 1322.590 kb on + strand, within bisCat 1322.591 kb on - strand, within bisCat 1322.591 kb on - strand, within bisCat 1322.591 kb on - strand, within bisCat 1322.598 kb on + strand, within bisCat 1322.598 kb on + strand, within bisCat 1322.598 kb on + strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.599 kb on - strand, within bisCat 1322.600 kb on + strand, within bisCat 1322.601 kb on - strand, within bisCat 1322.621 kb on + strand, within bisCat 1322.621 kb on + strand, within bisCat 1322.621 kb on + strand, within bisCat 1322.641 kb on - strand, within bisCat 1322.673 kb on + strand, within bisCat 1322.673 kb on + strand, within bisCat 1322.674 kb on - strand, within bisCat 1322.688 kb on - strand, within bisCat 1322.688 kb on - strand, within bisCat 1322.697 kb on + strand, within bisCat 1322.698 kb on - strand, within bisCat 1322.735 kb on + strand, within bisCat 1322.762 kb on + strand, within bisCat 1323.025 kb on - strand, within bisCat 1323.042 kb on + strand, within bisCat 1323.042 kb on + strand, within bisCat 1323.043 kb on - strand, within bisCat 1323.043 kb on - strand, within bisCat 1323.043 kb on - strand, within bisCat 1323.043 kb on - strand, within bisCat 1323.043 kb on - strand, within bisC

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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1,320,432 - ghrB MCAODC_06535 0.12 +0.0
1,320,439 - ghrB MCAODC_06535 0.13 +0.7
1,320,658 - ghrB MCAODC_06535 0.35 -0.3
1,320,693 + ghrB MCAODC_06535 0.39 +1.0
1,320,693 + ghrB MCAODC_06535 0.39 +0.3
1,320,694 - ghrB MCAODC_06535 0.39 -1.9
1,320,694 - ghrB MCAODC_06535 0.39 +0.0
1,320,694 - ghrB MCAODC_06535 0.39 +0.7
1,320,702 - ghrB MCAODC_06535 0.40 -1.4
1,320,746 + ghrB MCAODC_06535 0.45 +1.0
1,320,764 + ghrB MCAODC_06535 0.46 -1.1
1,320,766 + ghrB MCAODC_06535 0.47 -1.2
1,320,766 + ghrB MCAODC_06535 0.47 +0.2
1,320,767 - ghrB MCAODC_06535 0.47 -0.4
1,320,885 + ghrB MCAODC_06535 0.59 -1.7
1,320,885 + ghrB MCAODC_06535 0.59 -0.7
1,320,885 + ghrB MCAODC_06535 0.59 -1.9
1,320,913 - ghrB MCAODC_06535 0.62 -0.3
1,320,924 + ghrB MCAODC_06535 0.63 +0.2
1,320,991 - ghrB MCAODC_06535 0.70 -1.0
1,321,057 + ghrB MCAODC_06535 0.76 +0.1
1,321,058 - ghrB MCAODC_06535 0.77 -1.1
1,321,242 + +0.7
1,321,242 + +0.3
1,321,242 + -1.1
1,321,243 - -0.6
1,321,243 - +0.5
1,321,254 + -0.2
1,321,264 + +1.8
1,321,306 + +0.4
1,321,307 - -1.7
1,321,320 + -1.7
1,321,330 + +0.8
1,321,331 - -1.4
1,321,331 - +2.0
1,321,331 - +1.3
1,321,391 + -1.5
1,321,407 + -0.0
1,321,408 - -0.5
1,321,597 - yiaD MCAODC_06540 0.31 +0.0
1,321,598 + yiaD MCAODC_06540 0.32 +0.6
1,321,599 - yiaD MCAODC_06540 0.32 +1.5
1,321,599 - yiaD MCAODC_06540 0.32 -2.8
1,321,599 - yiaD MCAODC_06540 0.32 +1.3
1,321,599 - yiaD MCAODC_06540 0.32 -1.8
1,321,722 + yiaD MCAODC_06540 0.50 +0.5
1,321,732 - yiaD MCAODC_06540 0.52 +1.5
1,321,753 + yiaD MCAODC_06540 0.55 +0.3
1,321,758 + yiaD MCAODC_06540 0.56 +0.1
1,321,811 + yiaD MCAODC_06540 0.64 +0.1
1,321,811 + yiaD MCAODC_06540 0.64 -0.5
1,321,811 + yiaD MCAODC_06540 0.64 -0.6
1,321,811 + yiaD MCAODC_06540 0.64 -0.9
1,321,811 + yiaD MCAODC_06540 0.64 +0.0
1,321,811 + yiaD MCAODC_06540 0.64 -1.1
1,321,811 + yiaD MCAODC_06540 0.64 +1.1
1,321,811 + yiaD MCAODC_06540 0.64 -0.9
1,321,811 + yiaD MCAODC_06540 0.64 -0.0
1,321,812 - yiaD MCAODC_06540 0.64 -1.0
1,321,812 - yiaD MCAODC_06540 0.64 +0.2
1,321,812 - yiaD MCAODC_06540 0.64 +0.9
1,321,812 - yiaD MCAODC_06540 0.64 -1.0
1,321,812 - yiaD MCAODC_06540 0.64 +1.8
1,321,812 - yiaD MCAODC_06540 0.64 +0.5
1,321,812 - yiaD MCAODC_06540 0.64 -0.4
1,321,815 - yiaD MCAODC_06540 0.64 +1.0
1,321,815 - yiaD MCAODC_06540 0.64 +0.4
1,321,974 - yiaD MCAODC_06540 0.88 -1.3
1,321,974 - yiaD MCAODC_06540 0.88 -0.5
1,321,974 - yiaD MCAODC_06540 0.88 +0.2
1,321,980 + yiaD MCAODC_06540 0.89 -0.1
1,321,980 + yiaD MCAODC_06540 0.89 +1.3
1,321,981 - yiaD MCAODC_06540 0.90 +0.5
1,321,996 + -0.1
1,322,029 - -1.0
1,322,060 + -0.0
1,322,060 + -1.3
1,322,060 + -2.2
1,322,061 - +0.6
1,322,061 - -0.8
1,322,063 + +0.3
1,322,104 + +0.0
1,322,104 + -0.2
1,322,126 + -0.7
1,322,126 + -0.7
1,322,127 - -0.5
1,322,127 - +1.5
1,322,156 + +0.2
1,322,156 + +1.4
1,322,160 - -2.7
1,322,164 - +0.4
1,322,164 - -1.0
1,322,174 + -2.6
1,322,174 + +0.7
1,322,174 + +0.8
1,322,174 + -1.2
1,322,174 + +1.5
1,322,174 + -0.1
1,322,174 + -1.1
1,322,174 + -1.1
1,322,175 - -0.4
1,322,175 - -0.5
1,322,175 - -0.2
1,322,180 + -0.1
1,322,180 + -0.0
1,322,180 + +1.5
1,322,181 - -1.4
1,322,289 - -1.3
1,322,364 - -1.5
1,322,364 - +1.8
1,322,481 - bisC MCAODC_06545 0.12 +1.6
1,322,481 - bisC MCAODC_06545 0.12 -1.2
1,322,495 + bisC MCAODC_06545 0.13 -0.2
1,322,496 - bisC MCAODC_06545 0.13 -0.3
1,322,514 + bisC MCAODC_06545 0.13 -0.5
1,322,514 + bisC MCAODC_06545 0.13 -0.2
1,322,515 - bisC MCAODC_06545 0.13 -1.6
1,322,515 - bisC MCAODC_06545 0.13 -0.4
1,322,544 + bisC MCAODC_06545 0.15 +0.1
1,322,574 - bisC MCAODC_06545 0.16 -0.4
1,322,574 - bisC MCAODC_06545 0.16 +0.2
1,322,588 - bisC MCAODC_06545 0.17 +0.4
1,322,588 - bisC MCAODC_06545 0.17 +0.1
1,322,588 - bisC MCAODC_06545 0.17 +1.6
1,322,590 + bisC MCAODC_06545 0.17 -1.2
1,322,590 + bisC MCAODC_06545 0.17 -0.0
1,322,591 - bisC MCAODC_06545 0.17 -0.1
1,322,591 - bisC MCAODC_06545 0.17 -1.6
1,322,591 - bisC MCAODC_06545 0.17 -0.0
1,322,598 + bisC MCAODC_06545 0.17 +0.2
1,322,598 + bisC MCAODC_06545 0.17 +2.8
1,322,598 + bisC MCAODC_06545 0.17 +0.1
1,322,599 - bisC MCAODC_06545 0.17 -0.3
1,322,599 - bisC MCAODC_06545 0.17 -2.6
1,322,599 - bisC MCAODC_06545 0.17 -1.2
1,322,599 - bisC MCAODC_06545 0.17 -3.0
1,322,599 - bisC MCAODC_06545 0.17 -1.0
1,322,599 - bisC MCAODC_06545 0.17 +0.8
1,322,600 + bisC MCAODC_06545 0.17 +0.3
1,322,601 - bisC MCAODC_06545 0.17 +1.0
1,322,621 + bisC MCAODC_06545 0.18 +0.5
1,322,621 + bisC MCAODC_06545 0.18 -1.3
1,322,621 + bisC MCAODC_06545 0.18 -0.6
1,322,641 - bisC MCAODC_06545 0.19 +1.1
1,322,673 + bisC MCAODC_06545 0.20 -1.0
1,322,673 + bisC MCAODC_06545 0.20 -0.7
1,322,674 - bisC MCAODC_06545 0.20 -0.5
1,322,688 - bisC MCAODC_06545 0.21 -0.9
1,322,688 - bisC MCAODC_06545 0.21 +0.5
1,322,697 + bisC MCAODC_06545 0.21 -0.6
1,322,698 - bisC MCAODC_06545 0.21 -0.0
1,322,735 + bisC MCAODC_06545 0.23 -0.5
1,322,762 + bisC MCAODC_06545 0.24 +0.6
1,323,025 - bisC MCAODC_06545 0.35 +0.2
1,323,042 + bisC MCAODC_06545 0.36 -0.2
1,323,042 + bisC MCAODC_06545 0.36 -2.5
1,323,043 - bisC MCAODC_06545 0.36 -0.0
1,323,043 - bisC MCAODC_06545 0.36 +0.9
1,323,043 - bisC MCAODC_06545 0.36 +0.5
1,323,043 - bisC MCAODC_06545 0.36 -0.5
1,323,043 - bisC MCAODC_06545 0.36 +0.6

Or see this region's nucleotide sequence