Strain Fitness in Escherichia coli ECRC101 around MCAODC_06185

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotidesrfaJ and rfaB are separated by 8 nucleotides MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204 waaO MCAODC_06180: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,238,230 to 1,238,937 rfaY MCAODC_06185: rfaJ - glucosyltransferase, at 1,238,962 to 1,239,975 rfaJ MCAODC_06190: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 1,239,984 to 1,241,126 rfaB Position (kb) 1238 1239 1240Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1237.981 kb on + strand, within waaOat 1238.087 kb on - strand, within waaOat 1238.211 kb on - strandat 1238.402 kb on + strand, within rfaYat 1238.673 kb on - strand, within rfaYat 1238.825 kb on - strand, within rfaYat 1238.859 kb on + strand, within rfaYat 1238.996 kb on + strandat 1239.045 kb on + strandat 1239.183 kb on - strand, within rfaJat 1239.338 kb on + strand, within rfaJat 1239.344 kb on - strand, within rfaJat 1239.434 kb on + strand, within rfaJat 1239.435 kb on - strand, within rfaJat 1239.441 kb on + strand, within rfaJat 1239.441 kb on + strand, within rfaJat 1239.493 kb on + strand, within rfaJat 1239.514 kb on + strand, within rfaJat 1239.722 kb on - strand, within rfaJat 1239.835 kb on - strand, within rfaJat 1239.837 kb on - strand, within rfaJat 1239.887 kb on - strandat 1240.304 kb on + strand, within rfaBat 1240.364 kb on + strand, within rfaBat 1240.426 kb on + strand, within rfaBat 1240.544 kb on + strand, within rfaBat 1240.683 kb on - strand, within rfaBat 1240.763 kb on + strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.764 kb on - strand, within rfaBat 1240.768 kb on + strand, within rfaBat 1240.800 kb on + strand, within rfaBat 1240.875 kb on - strand, within rfaBat 1240.886 kb on + strand, within rfaBat 1240.886 kb on + strand, within rfaBat 1240.967 kb on + strand, within rfaB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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1,237,981 + waaO MCAODC_06175 0.78 -0.3
1,238,087 - waaO MCAODC_06175 0.88 +1.0
1,238,211 - -2.6
1,238,402 + rfaY MCAODC_06180 0.24 +0.2
1,238,673 - rfaY MCAODC_06180 0.63 -0.6
1,238,825 - rfaY MCAODC_06180 0.84 -0.2
1,238,859 + rfaY MCAODC_06180 0.89 +1.5
1,238,996 + -3.3
1,239,045 + -1.8
1,239,183 - rfaJ MCAODC_06185 0.22 -3.3
1,239,338 + rfaJ MCAODC_06185 0.37 -0.2
1,239,344 - rfaJ MCAODC_06185 0.38 +0.7
1,239,434 + rfaJ MCAODC_06185 0.47 -3.9
1,239,435 - rfaJ MCAODC_06185 0.47 -2.7
1,239,441 + rfaJ MCAODC_06185 0.47 -1.9
1,239,441 + rfaJ MCAODC_06185 0.47 -3.5
1,239,493 + rfaJ MCAODC_06185 0.52 -3.3
1,239,514 + rfaJ MCAODC_06185 0.54 +0.5
1,239,722 - rfaJ MCAODC_06185 0.75 -2.0
1,239,835 - rfaJ MCAODC_06185 0.86 -3.5
1,239,837 - rfaJ MCAODC_06185 0.86 -4.1
1,239,887 - -3.4
1,240,304 + rfaB MCAODC_06190 0.28 +0.8
1,240,364 + rfaB MCAODC_06190 0.33 -0.7
1,240,426 + rfaB MCAODC_06190 0.39 -0.7
1,240,544 + rfaB MCAODC_06190 0.49 -2.5
1,240,683 - rfaB MCAODC_06190 0.61 +2.1
1,240,763 + rfaB MCAODC_06190 0.68 +0.8
1,240,764 - rfaB MCAODC_06190 0.68 +0.1
1,240,764 - rfaB MCAODC_06190 0.68 -0.7
1,240,764 - rfaB MCAODC_06190 0.68 +0.5
1,240,768 + rfaB MCAODC_06190 0.69 +1.1
1,240,800 + rfaB MCAODC_06190 0.71 +0.6
1,240,875 - rfaB MCAODC_06190 0.78 +0.3
1,240,886 + rfaB MCAODC_06190 0.79 +1.7
1,240,886 + rfaB MCAODC_06190 0.79 +0.4
1,240,967 + rfaB MCAODC_06190 0.86 +0.1

Or see this region's nucleotide sequence