Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_03500 and MCAODC_03505 are separated by 86 nucleotides MCAODC_03505 and yfdR are separated by 127 nucleotides yfdR and MCAODC_03515 overlap by 10 nucleotides MCAODC_03515 and MCAODC_03520 overlap by 4 nucleotides MCAODC_03520 and MCAODC_03525 are separated by 335 nucleotides
MCAODC_03500: MCAODC_03500 - phage tail protein, at 705,323 to 705,976
_03500
MCAODC_03505: MCAODC_03505 - CPS-53 (KpLE1) prophage protein, at 706,063 to 706,449
_03505
MCAODC_03510: yfdR - 5'-deoxynucleotidase, at 706,577 to 707,113
yfdR
MCAODC_03515: MCAODC_03515 - Eae protein, at 707,104 to 707,454
_03515
MCAODC_03520: MCAODC_03520 - SAM-dependent methyltransferase, at 707,451 to 707,735
_03520
MCAODC_03525: MCAODC_03525 - hypothetical protein, at 708,071 to 708,268
_03525
Position (kb) 706
707
708 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 706.057 kb on + strand at 706.057 kb on + strand at 706.078 kb on - strand at 706.165 kb on - strand, within MCAODC_03505 at 706.225 kb on - strand, within MCAODC_03505 at 706.339 kb on - strand, within MCAODC_03505 at 706.493 kb on + strand at 706.494 kb on - strand at 706.505 kb on + strand at 706.534 kb on - strand at 706.562 kb on + strand at 706.562 kb on + strand at 706.563 kb on - strand at 706.563 kb on - strand at 706.630 kb on + strand at 706.676 kb on - strand, within yfdR at 706.776 kb on + strand, within yfdR at 706.800 kb on - strand, within yfdR at 706.800 kb on - strand, within yfdR at 706.844 kb on + strand, within yfdR at 706.845 kb on - strand, within yfdR at 706.845 kb on - strand, within yfdR at 706.845 kb on - strand, within yfdR at 706.845 kb on - strand, within yfdR at 706.846 kb on + strand, within yfdR at 706.847 kb on - strand, within yfdR at 706.906 kb on + strand, within yfdR at 706.907 kb on - strand, within yfdR at 706.925 kb on + strand, within yfdR at 706.936 kb on + strand, within yfdR at 706.936 kb on + strand, within yfdR at 706.936 kb on + strand, within yfdR at 706.937 kb on - strand, within yfdR at 706.937 kb on - strand, within yfdR at 706.995 kb on + strand, within yfdR at 707.047 kb on + strand, within yfdR at 707.047 kb on + strand, within yfdR at 707.047 kb on + strand, within yfdR at 707.047 kb on + strand, within yfdR at 707.047 kb on + strand, within yfdR at 707.068 kb on + strand at 707.081 kb on - strand at 707.097 kb on + strand at 707.098 kb on - strand at 707.191 kb on + strand, within MCAODC_03515 at 707.191 kb on + strand, within MCAODC_03515 at 707.217 kb on - strand, within MCAODC_03515 at 707.217 kb on - strand, within MCAODC_03515 at 707.217 kb on - strand, within MCAODC_03515 at 707.241 kb on + strand, within MCAODC_03515 at 707.241 kb on + strand, within MCAODC_03515 at 707.242 kb on - strand, within MCAODC_03515 at 707.244 kb on + strand, within MCAODC_03515 at 707.244 kb on + strand, within MCAODC_03515 at 707.244 kb on + strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.245 kb on - strand, within MCAODC_03515 at 707.248 kb on + strand, within MCAODC_03515 at 707.249 kb on - strand, within MCAODC_03515 at 707.249 kb on - strand, within MCAODC_03515 at 707.290 kb on - strand, within MCAODC_03515 at 707.290 kb on - strand, within MCAODC_03515 at 707.296 kb on - strand, within MCAODC_03515 at 707.354 kb on + strand, within MCAODC_03515 at 707.354 kb on + strand, within MCAODC_03515 at 707.354 kb on + strand, within MCAODC_03515 at 707.355 kb on - strand, within MCAODC_03515 at 707.358 kb on + strand, within MCAODC_03515 at 707.359 kb on - strand, within MCAODC_03515 at 707.359 kb on - strand, within MCAODC_03515 at 707.359 kb on - strand, within MCAODC_03515 at 707.396 kb on - strand, within MCAODC_03515 at 707.585 kb on + strand, within MCAODC_03520 at 707.603 kb on - strand, within MCAODC_03520 at 707.620 kb on + strand, within MCAODC_03520 at 707.620 kb on + strand, within MCAODC_03520 at 707.620 kb on + strand, within MCAODC_03520 at 707.621 kb on - strand, within MCAODC_03520 at 707.652 kb on - strand, within MCAODC_03520 at 707.653 kb on + strand, within MCAODC_03520 at 707.654 kb on - strand, within MCAODC_03520 at 707.709 kb on + strand at 707.824 kb on + strand at 707.839 kb on + strand at 707.845 kb on + strand at 707.910 kb on - strand at 707.957 kb on + strand at 707.961 kb on + strand at 707.961 kb on + strand at 707.962 kb on - strand at 707.963 kb on + strand at 707.975 kb on + strand at 707.976 kb on - strand at 708.094 kb on - strand, within MCAODC_03525 at 708.094 kb on - strand, within MCAODC_03525
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 706,057 + +0.5 706,057 + +0.3 706,078 - +0.9 706,165 - MCAODC_03505 0.26 +0.8 706,225 - MCAODC_03505 0.42 -0.2 706,339 - MCAODC_03505 0.71 -0.2 706,493 + -1.9 706,494 - -1.0 706,505 + -1.1 706,534 - -1.0 706,562 + -0.5 706,562 + +0.4 706,563 - +1.3 706,563 - -1.5 706,630 + -0.5 706,676 - yfdR MCAODC_03510 0.18 -0.2 706,776 + yfdR MCAODC_03510 0.37 -0.8 706,800 - yfdR MCAODC_03510 0.42 -0.8 706,800 - yfdR MCAODC_03510 0.42 +0.3 706,844 + yfdR MCAODC_03510 0.50 -0.4 706,845 - yfdR MCAODC_03510 0.50 -0.1 706,845 - yfdR MCAODC_03510 0.50 +0.5 706,845 - yfdR MCAODC_03510 0.50 -1.0 706,845 - yfdR MCAODC_03510 0.50 -0.7 706,846 + yfdR MCAODC_03510 0.50 -1.4 706,847 - yfdR MCAODC_03510 0.50 +0.1 706,906 + yfdR MCAODC_03510 0.61 -0.3 706,907 - yfdR MCAODC_03510 0.61 +0.3 706,925 + yfdR MCAODC_03510 0.65 +1.5 706,936 + yfdR MCAODC_03510 0.67 -0.3 706,936 + yfdR MCAODC_03510 0.67 -0.1 706,936 + yfdR MCAODC_03510 0.67 +0.6 706,937 - yfdR MCAODC_03510 0.67 -1.3 706,937 - yfdR MCAODC_03510 0.67 -0.1 706,995 + yfdR MCAODC_03510 0.78 +0.3 707,047 + yfdR MCAODC_03510 0.88 +1.0 707,047 + yfdR MCAODC_03510 0.88 -1.0 707,047 + yfdR MCAODC_03510 0.88 +0.6 707,047 + yfdR MCAODC_03510 0.88 +2.8 707,047 + yfdR MCAODC_03510 0.88 -0.9 707,068 + -0.4 707,081 - -1.3 707,097 + +0.0 707,098 - +2.1 707,191 + MCAODC_03515 0.25 -0.0 707,191 + MCAODC_03515 0.25 +2.1 707,217 - MCAODC_03515 0.32 +1.4 707,217 - MCAODC_03515 0.32 -0.6 707,217 - MCAODC_03515 0.32 +0.5 707,241 + MCAODC_03515 0.39 +0.1 707,241 + MCAODC_03515 0.39 -0.1 707,242 - MCAODC_03515 0.39 -1.2 707,244 + MCAODC_03515 0.40 -0.2 707,244 + MCAODC_03515 0.40 -0.0 707,244 + MCAODC_03515 0.40 +0.9 707,245 - MCAODC_03515 0.40 -0.7 707,245 - MCAODC_03515 0.40 -0.6 707,245 - MCAODC_03515 0.40 +0.0 707,245 - MCAODC_03515 0.40 +0.9 707,245 - MCAODC_03515 0.40 +0.0 707,245 - MCAODC_03515 0.40 -0.2 707,245 - MCAODC_03515 0.40 -1.0 707,245 - MCAODC_03515 0.40 -0.2 707,245 - MCAODC_03515 0.40 +1.9 707,245 - MCAODC_03515 0.40 -0.3 707,245 - MCAODC_03515 0.40 -0.5 707,245 - MCAODC_03515 0.40 +0.8 707,248 + MCAODC_03515 0.41 +0.3 707,249 - MCAODC_03515 0.41 +0.6 707,249 - MCAODC_03515 0.41 -1.0 707,290 - MCAODC_03515 0.53 -0.5 707,290 - MCAODC_03515 0.53 -0.4 707,296 - MCAODC_03515 0.55 -0.9 707,354 + MCAODC_03515 0.71 +0.9 707,354 + MCAODC_03515 0.71 +0.2 707,354 + MCAODC_03515 0.71 -1.0 707,355 - MCAODC_03515 0.72 +0.4 707,358 + MCAODC_03515 0.72 +0.0 707,359 - MCAODC_03515 0.73 -0.0 707,359 - MCAODC_03515 0.73 +2.9 707,359 - MCAODC_03515 0.73 +1.6 707,396 - MCAODC_03515 0.83 -0.6 707,585 + MCAODC_03520 0.47 -0.5 707,603 - MCAODC_03520 0.53 +1.0 707,620 + MCAODC_03520 0.59 +1.1 707,620 + MCAODC_03520 0.59 -0.9 707,620 + MCAODC_03520 0.59 -0.9 707,621 - MCAODC_03520 0.60 -2.4 707,652 - MCAODC_03520 0.71 -0.9 707,653 + MCAODC_03520 0.71 -0.5 707,654 - MCAODC_03520 0.71 -0.9 707,709 + +0.3 707,824 + +0.3 707,839 + -1.1 707,845 + +0.2 707,910 - -1.8 707,957 + -2.0 707,961 + +0.6 707,961 + -0.7 707,962 - +0.4 707,963 + -0.1 707,975 + -1.6 707,976 - -0.3 708,094 - MCAODC_03525 0.12 +1.2 708,094 - MCAODC_03525 0.12 -1.4
Or see this region's nucleotide sequence