Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsdR and hsdM are separated by 66 nucleotides hsdM and hsdS overlap by 1 nucleotides
MCAODC_01925: hsdR - DEAD/DEAH box helicase, at 372,066 to 374,498
hsdR
MCAODC_01930: hsdM - Type I restriction enzyme EcoEI methylase subunit, at 374,565 to 376,034
hsdM
MCAODC_01935: hsdS - restriction endonuclease subunit S, at 376,034 to 377,788
hsdS
Position (kb) 374
375
376
377 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 373.577 kb on - strand, within hsdR at 373.635 kb on - strand, within hsdR at 373.671 kb on - strand, within hsdR at 373.831 kb on + strand, within hsdR at 373.878 kb on - strand, within hsdR at 373.945 kb on + strand, within hsdR at 373.977 kb on + strand, within hsdR at 373.977 kb on + strand, within hsdR at 373.978 kb on - strand, within hsdR at 373.980 kb on + strand, within hsdR at 374.023 kb on - strand, within hsdR at 374.023 kb on - strand, within hsdR at 374.146 kb on + strand, within hsdR at 374.165 kb on + strand, within hsdR at 374.200 kb on + strand, within hsdR at 374.200 kb on + strand, within hsdR at 374.200 kb on + strand, within hsdR at 374.201 kb on - strand, within hsdR at 374.205 kb on + strand, within hsdR at 374.205 kb on + strand, within hsdR at 374.205 kb on + strand, within hsdR at 374.206 kb on - strand, within hsdR at 374.206 kb on - strand, within hsdR at 374.206 kb on - strand, within hsdR at 374.206 kb on - strand, within hsdR at 374.206 kb on - strand, within hsdR at 374.262 kb on + strand at 374.263 kb on - strand at 374.274 kb on + strand at 374.274 kb on + strand at 374.320 kb on - strand at 374.351 kb on + strand at 374.406 kb on - strand at 374.406 kb on - strand at 374.408 kb on + strand at 374.408 kb on + strand at 374.409 kb on - strand at 374.438 kb on + strand at 374.439 kb on - strand at 374.472 kb on + strand at 374.473 kb on - strand at 374.473 kb on - strand at 374.560 kb on - strand at 374.571 kb on - strand at 374.594 kb on - strand at 374.600 kb on + strand at 374.600 kb on + strand at 374.600 kb on + strand at 374.601 kb on - strand at 374.601 kb on - strand at 374.601 kb on - strand at 374.601 kb on - strand at 374.603 kb on + strand at 374.717 kb on + strand, within hsdM at 374.717 kb on + strand, within hsdM at 374.717 kb on + strand, within hsdM at 374.718 kb on - strand, within hsdM at 374.721 kb on + strand, within hsdM at 374.745 kb on + strand, within hsdM at 374.746 kb on - strand, within hsdM at 374.746 kb on - strand, within hsdM at 374.746 kb on - strand, within hsdM at 374.749 kb on + strand, within hsdM at 374.904 kb on + strand, within hsdM at 374.904 kb on + strand, within hsdM at 374.904 kb on + strand, within hsdM at 374.904 kb on + strand, within hsdM at 374.905 kb on - strand, within hsdM at 374.905 kb on - strand, within hsdM at 374.905 kb on - strand, within hsdM at 374.905 kb on - strand, within hsdM at 374.905 kb on - strand, within hsdM at 374.906 kb on + strand, within hsdM at 374.906 kb on + strand, within hsdM at 374.907 kb on - strand, within hsdM at 374.911 kb on - strand, within hsdM at 374.911 kb on - strand, within hsdM at 374.911 kb on - strand, within hsdM at 374.929 kb on + strand, within hsdM at 374.930 kb on - strand, within hsdM at 374.948 kb on + strand, within hsdM at 374.949 kb on - strand, within hsdM at 374.969 kb on + strand, within hsdM at 375.003 kb on - strand, within hsdM at 375.006 kb on + strand, within hsdM at 375.006 kb on + strand, within hsdM at 375.007 kb on - strand, within hsdM at 375.058 kb on - strand, within hsdM at 375.060 kb on - strand, within hsdM at 375.083 kb on + strand, within hsdM at 375.083 kb on + strand, within hsdM at 375.122 kb on + strand, within hsdM at 375.175 kb on + strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.187 kb on - strand, within hsdM at 375.278 kb on + strand, within hsdM at 375.278 kb on + strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.377 kb on + strand, within hsdM at 375.488 kb on - strand, within hsdM at 375.488 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.571 kb on + strand, within hsdM at 375.596 kb on + strand, within hsdM at 375.596 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.700 kb on - strand, within hsdM at 375.700 kb on - strand, within hsdM at 375.725 kb on - strand, within hsdM at 375.887 kb on + strand, within hsdM at 375.887 kb on + strand, within hsdM at 375.945 kb on + strand at 375.946 kb on - strand at 375.946 kb on - strand at 376.029 kb on - strand at 376.029 kb on - strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.033 kb on - strand at 376.134 kb on + strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.136 kb on + strand at 376.136 kb on + strand at 376.186 kb on + strand at 376.309 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.361 kb on - strand, within hsdS at 376.412 kb on - strand, within hsdS at 376.659 kb on - strand, within hsdS at 376.691 kb on + strand, within hsdS at 376.692 kb on - strand, within hsdS at 376.816 kb on - strand, within hsdS at 376.949 kb on - strand, within hsdS at 376.959 kb on - strand, within hsdS at 376.971 kb on + strand, within hsdS at 376.971 kb on + strand, within hsdS at 376.972 kb on - strand, within hsdS at 376.972 kb on - strand, within hsdS at 376.972 kb on - strand, within hsdS at 377.029 kb on - strand, within hsdS
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 373,577 - hsdR MCAODC_01925 0.62 -1.7 373,635 - hsdR MCAODC_01925 0.64 -0.5 373,671 - hsdR MCAODC_01925 0.66 -0.6 373,831 + hsdR MCAODC_01925 0.73 -3.0 373,878 - hsdR MCAODC_01925 0.74 -0.8 373,945 + hsdR MCAODC_01925 0.77 -0.6 373,977 + hsdR MCAODC_01925 0.79 -0.6 373,977 + hsdR MCAODC_01925 0.79 +0.7 373,978 - hsdR MCAODC_01925 0.79 -0.0 373,980 + hsdR MCAODC_01925 0.79 +0.7 374,023 - hsdR MCAODC_01925 0.80 -1.5 374,023 - hsdR MCAODC_01925 0.80 -0.4 374,146 + hsdR MCAODC_01925 0.85 -0.5 374,165 + hsdR MCAODC_01925 0.86 -0.7 374,200 + hsdR MCAODC_01925 0.88 -1.8 374,200 + hsdR MCAODC_01925 0.88 -0.8 374,200 + hsdR MCAODC_01925 0.88 +0.9 374,201 - hsdR MCAODC_01925 0.88 +1.0 374,205 + hsdR MCAODC_01925 0.88 -0.2 374,205 + hsdR MCAODC_01925 0.88 -0.7 374,205 + hsdR MCAODC_01925 0.88 +0.7 374,206 - hsdR MCAODC_01925 0.88 +0.7 374,206 - hsdR MCAODC_01925 0.88 -1.1 374,206 - hsdR MCAODC_01925 0.88 +0.8 374,206 - hsdR MCAODC_01925 0.88 -0.8 374,206 - hsdR MCAODC_01925 0.88 +0.3 374,262 + +0.5 374,263 - +0.8 374,274 + -0.9 374,274 + +0.5 374,320 - -0.3 374,351 + -0.3 374,406 - -2.3 374,406 - -0.5 374,408 + -1.9 374,408 + +0.9 374,409 - -0.7 374,438 + +1.5 374,439 - -0.5 374,472 + -0.9 374,473 - -0.4 374,473 - +1.0 374,560 - -0.6 374,571 - -0.3 374,594 - +0.3 374,600 + -0.7 374,600 + -1.2 374,600 + -0.3 374,601 - -0.2 374,601 - +1.5 374,601 - -1.1 374,601 - -0.1 374,603 + -0.1 374,717 + hsdM MCAODC_01930 0.10 -0.4 374,717 + hsdM MCAODC_01930 0.10 +0.1 374,717 + hsdM MCAODC_01930 0.10 -0.6 374,718 - hsdM MCAODC_01930 0.10 +0.8 374,721 + hsdM MCAODC_01930 0.11 +1.1 374,745 + hsdM MCAODC_01930 0.12 -0.1 374,746 - hsdM MCAODC_01930 0.12 -0.1 374,746 - hsdM MCAODC_01930 0.12 -1.1 374,746 - hsdM MCAODC_01930 0.12 -0.5 374,749 + hsdM MCAODC_01930 0.13 +0.2 374,904 + hsdM MCAODC_01930 0.23 -1.2 374,904 + hsdM MCAODC_01930 0.23 -0.0 374,904 + hsdM MCAODC_01930 0.23 -0.6 374,904 + hsdM MCAODC_01930 0.23 -0.8 374,905 - hsdM MCAODC_01930 0.23 -0.6 374,905 - hsdM MCAODC_01930 0.23 +0.1 374,905 - hsdM MCAODC_01930 0.23 -0.0 374,905 - hsdM MCAODC_01930 0.23 +0.6 374,905 - hsdM MCAODC_01930 0.23 -0.6 374,906 + hsdM MCAODC_01930 0.23 -2.2 374,906 + hsdM MCAODC_01930 0.23 -1.0 374,907 - hsdM MCAODC_01930 0.23 +0.1 374,911 - hsdM MCAODC_01930 0.24 -0.3 374,911 - hsdM MCAODC_01930 0.24 +1.3 374,911 - hsdM MCAODC_01930 0.24 +1.5 374,929 + hsdM MCAODC_01930 0.25 +0.3 374,930 - hsdM MCAODC_01930 0.25 +1.3 374,948 + hsdM MCAODC_01930 0.26 -0.9 374,949 - hsdM MCAODC_01930 0.26 +0.1 374,969 + hsdM MCAODC_01930 0.27 +0.5 375,003 - hsdM MCAODC_01930 0.30 -0.5 375,006 + hsdM MCAODC_01930 0.30 -1.1 375,006 + hsdM MCAODC_01930 0.30 -0.8 375,007 - hsdM MCAODC_01930 0.30 -1.9 375,058 - hsdM MCAODC_01930 0.34 -1.2 375,060 - hsdM MCAODC_01930 0.34 +0.2 375,083 + hsdM MCAODC_01930 0.35 -0.1 375,083 + hsdM MCAODC_01930 0.35 +2.5 375,122 + hsdM MCAODC_01930 0.38 -0.3 375,175 + hsdM MCAODC_01930 0.41 +2.4 375,176 - hsdM MCAODC_01930 0.42 -0.8 375,176 - hsdM MCAODC_01930 0.42 +0.1 375,176 - hsdM MCAODC_01930 0.42 -0.8 375,187 - hsdM MCAODC_01930 0.42 -0.6 375,278 + hsdM MCAODC_01930 0.49 +1.0 375,278 + hsdM MCAODC_01930 0.49 -0.7 375,279 - hsdM MCAODC_01930 0.49 -1.1 375,279 - hsdM MCAODC_01930 0.49 -0.2 375,279 - hsdM MCAODC_01930 0.49 -0.7 375,377 + hsdM MCAODC_01930 0.55 -0.6 375,488 - hsdM MCAODC_01930 0.63 -1.0 375,488 - hsdM MCAODC_01930 0.63 -1.6 375,536 - hsdM MCAODC_01930 0.66 -0.7 375,536 - hsdM MCAODC_01930 0.66 -0.2 375,536 - hsdM MCAODC_01930 0.66 -0.0 375,571 + hsdM MCAODC_01930 0.68 -2.4 375,596 + hsdM MCAODC_01930 0.70 +0.3 375,596 + hsdM MCAODC_01930 0.70 -0.4 375,699 + hsdM MCAODC_01930 0.77 -1.0 375,699 + hsdM MCAODC_01930 0.77 +0.5 375,699 + hsdM MCAODC_01930 0.77 +1.0 375,700 - hsdM MCAODC_01930 0.77 +0.0 375,700 - hsdM MCAODC_01930 0.77 -0.6 375,725 - hsdM MCAODC_01930 0.79 +0.1 375,887 + hsdM MCAODC_01930 0.90 +0.3 375,887 + hsdM MCAODC_01930 0.90 -1.1 375,945 + +0.2 375,946 - -0.3 375,946 - +0.3 376,029 - +0.0 376,029 - +0.8 376,032 + +0.6 376,032 + -0.3 376,032 + -0.4 376,032 + -1.3 376,032 + -1.3 376,033 - -2.3 376,134 + +1.5 376,135 - -1.9 376,135 - +1.8 376,135 - +0.3 376,135 - -1.3 376,135 - +0.9 376,136 + +1.3 376,136 + -1.5 376,186 + -0.9 376,309 + hsdS MCAODC_01935 0.16 -0.9 376,360 + hsdS MCAODC_01935 0.19 +0.0 376,360 + hsdS MCAODC_01935 0.19 +0.4 376,360 + hsdS MCAODC_01935 0.19 -0.6 376,361 - hsdS MCAODC_01935 0.19 +1.2 376,412 - hsdS MCAODC_01935 0.22 +0.8 376,659 - hsdS MCAODC_01935 0.36 -0.4 376,691 + hsdS MCAODC_01935 0.37 -1.3 376,692 - hsdS MCAODC_01935 0.37 +0.3 376,816 - hsdS MCAODC_01935 0.45 -0.3 376,949 - hsdS MCAODC_01935 0.52 +0.1 376,959 - hsdS MCAODC_01935 0.53 +1.1 376,971 + hsdS MCAODC_01935 0.53 -0.3 376,971 + hsdS MCAODC_01935 0.53 -0.6 376,972 - hsdS MCAODC_01935 0.53 +3.8 376,972 - hsdS MCAODC_01935 0.53 +0.7 376,972 - hsdS MCAODC_01935 0.53 +0.8 377,029 - hsdS MCAODC_01935 0.57 -2.3
Or see this region's nucleotide sequence