Strain Fitness in Escherichia coli ECRC101 around MCAODC_27035

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_27025 and ybcJ are separated by 1 nucleotidesybcJ and ybcI are separated by 107 nucleotidesybcI and cysS are separated by 35 nucleotides MCAODC_27025: MCAODC_27025 - Bifunctional protein FolD, at 2,130,608 to 2,131,474 _27025 MCAODC_27030: ybcJ - ribosome-associated protein YbcJ, at 2,131,476 to 2,131,688 ybcJ MCAODC_27035: ybcI - Inner membrane protein YbcI, at 2,131,796 to 2,132,317 ybcI MCAODC_27040: cysS - cysteine--tRNA ligase, at 2,132,353 to 2,133,738 cysS Position (kb) 2131 2132 2133Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2131.434 kb on + strandat 2131.452 kb on - strandat 2131.492 kb on + strandat 2131.493 kb on - strandat 2131.496 kb on + strandat 2131.496 kb on + strandat 2131.497 kb on - strandat 2131.766 kb on + strandat 2131.767 kb on - strandat 2131.767 kb on - strandat 2131.767 kb on - strandat 2131.810 kb on + strandat 2131.810 kb on + strandat 2131.810 kb on + strandat 2131.810 kb on + strandat 2131.811 kb on - strandat 2131.842 kb on + strandat 2131.878 kb on + strand, within ybcIat 2131.879 kb on - strand, within ybcIat 2131.930 kb on + strand, within ybcIat 2131.975 kb on + strand, within ybcIat 2132.013 kb on + strand, within ybcIat 2132.014 kb on - strand, within ybcIat 2132.073 kb on + strand, within ybcIat 2132.189 kb on - strand, within ybcIat 2132.229 kb on - strand, within ybcIat 2132.283 kb on + strandat 2132.284 kb on - strandat 2132.346 kb on - strandat 2132.346 kb on - strandat 2132.355 kb on - strandat 2132.358 kb on + strandat 2132.359 kb on - strandat 2132.359 kb on - strandat 2132.359 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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2,131,434 + -2.3
2,131,452 - -0.7
2,131,492 + -0.5
2,131,493 - -1.8
2,131,496 + -0.4
2,131,496 + -0.2
2,131,497 - -2.9
2,131,766 + -0.3
2,131,767 - -0.7
2,131,767 - +0.2
2,131,767 - -0.5
2,131,810 + -0.2
2,131,810 + -0.3
2,131,810 + -0.9
2,131,810 + +0.3
2,131,811 - -1.4
2,131,842 + +0.4
2,131,878 + ybcI MCAODC_27035 0.16 -1.3
2,131,879 - ybcI MCAODC_27035 0.16 +0.2
2,131,930 + ybcI MCAODC_27035 0.26 +0.2
2,131,975 + ybcI MCAODC_27035 0.34 +0.3
2,132,013 + ybcI MCAODC_27035 0.42 +1.6
2,132,014 - ybcI MCAODC_27035 0.42 -0.0
2,132,073 + ybcI MCAODC_27035 0.53 +0.5
2,132,189 - ybcI MCAODC_27035 0.75 +1.0
2,132,229 - ybcI MCAODC_27035 0.83 +2.0
2,132,283 + +0.6
2,132,284 - +2.1
2,132,346 - +2.1
2,132,346 - +0.7
2,132,355 - +0.4
2,132,358 + +0.6
2,132,359 - -0.5
2,132,359 - -0.5
2,132,359 - -1.4

Or see this region's nucleotide sequence