Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgD and flgC are separated by 11 nucleotides flgC and flgB are separated by 3 nucleotides flgB and flgA are separated by 154 nucleotides flgA and flgM are separated by 75 nucleotides
MCAODC_23025: flgD - flagellar hook assembly protein FlgD, at 1,348,786 to 1,349,481
flgD
MCAODC_23030: flgC - flagellar basal body rod protein FlgC, at 1,349,493 to 1,349,897
flgC
MCAODC_23035: flgB - flagellar basal body rod protein FlgB, at 1,349,901 to 1,350,317
flgB
MCAODC_23040: flgA - flagellar basal body P-ring formation protein FlgA, at 1,350,472 to 1,351,131
flgA
MCAODC_23045: flgM - anti-sigma-28 factor FlgM, at 1,351,207 to 1,351,500
flgM
Position (kb)
1349
1350
1351 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1348.915 kb on - strand, within flgD at 1348.983 kb on + strand, within flgD at 1348.984 kb on - strand, within flgD at 1349.226 kb on + strand, within flgD at 1349.245 kb on - strand, within flgD at 1349.422 kb on - strand at 1349.422 kb on - strand at 1349.422 kb on - strand at 1349.445 kb on + strand at 1349.481 kb on + strand at 1349.482 kb on - strand at 1349.482 kb on - strand at 1349.482 kb on - strand at 1349.482 kb on - strand at 1349.482 kb on - strand at 1349.563 kb on + strand, within flgC at 1349.695 kb on - strand, within flgC at 1349.695 kb on - strand, within flgC at 1349.695 kb on - strand, within flgC at 1349.695 kb on - strand, within flgC at 1349.696 kb on + strand, within flgC at 1349.801 kb on + strand, within flgC at 1349.917 kb on - strand at 1349.957 kb on + strand, within flgB at 1349.972 kb on - strand, within flgB at 1350.024 kb on - strand, within flgB at 1350.053 kb on + strand, within flgB at 1350.317 kb on + strand at 1350.317 kb on + strand at 1350.318 kb on - strand at 1350.355 kb on - strand at 1350.355 kb on - strand at 1350.405 kb on + strand at 1350.406 kb on - strand at 1350.406 kb on - strand at 1350.409 kb on + strand at 1350.410 kb on - strand at 1350.437 kb on + strand at 1350.451 kb on + strand at 1350.489 kb on + strand at 1350.587 kb on + strand, within flgA at 1350.588 kb on - strand, within flgA at 1350.615 kb on + strand, within flgA at 1350.615 kb on + strand, within flgA at 1350.629 kb on + strand, within flgA at 1350.629 kb on + strand, within flgA at 1350.630 kb on - strand, within flgA at 1350.630 kb on - strand, within flgA at 1350.669 kb on + strand, within flgA at 1350.669 kb on + strand, within flgA at 1350.727 kb on + strand, within flgA at 1350.728 kb on - strand, within flgA at 1350.731 kb on + strand, within flgA at 1350.841 kb on - strand, within flgA at 1350.905 kb on + strand, within flgA at 1351.082 kb on + strand at 1351.083 kb on - strand at 1351.120 kb on - strand at 1351.122 kb on - strand at 1351.165 kb on + strand at 1351.165 kb on + strand at 1351.179 kb on + strand at 1351.202 kb on - strand at 1351.212 kb on + strand at 1351.212 kb on + strand at 1351.241 kb on + strand, within flgM
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,348,915 - flgD MCAODC_23025 0.19 +1.2 1,348,983 + flgD MCAODC_23025 0.28 +2.0 1,348,984 - flgD MCAODC_23025 0.28 +1.8 1,349,226 + flgD MCAODC_23025 0.63 -0.4 1,349,245 - flgD MCAODC_23025 0.66 -1.9 1,349,422 - +0.6 1,349,422 - +1.4 1,349,422 - -0.1 1,349,445 + -1.8 1,349,481 + +0.6 1,349,482 - +0.7 1,349,482 - -0.2 1,349,482 - +0.1 1,349,482 - -0.9 1,349,482 - -0.2 1,349,563 + flgC MCAODC_23030 0.17 -1.3 1,349,695 - flgC MCAODC_23030 0.50 +0.5 1,349,695 - flgC MCAODC_23030 0.50 +0.0 1,349,695 - flgC MCAODC_23030 0.50 -1.7 1,349,695 - flgC MCAODC_23030 0.50 -2.9 1,349,696 + flgC MCAODC_23030 0.50 -0.6 1,349,801 + flgC MCAODC_23030 0.76 +1.0 1,349,917 - +1.1 1,349,957 + flgB MCAODC_23035 0.13 +0.8 1,349,972 - flgB MCAODC_23035 0.17 +0.8 1,350,024 - flgB MCAODC_23035 0.29 -0.4 1,350,053 + flgB MCAODC_23035 0.36 -0.6 1,350,317 + +0.8 1,350,317 + +1.0 1,350,318 - -1.5 1,350,355 - +0.4 1,350,355 - +0.4 1,350,405 + +2.4 1,350,406 - +0.8 1,350,406 - -0.2 1,350,409 + +0.4 1,350,410 - -2.3 1,350,437 + -0.3 1,350,451 + +0.5 1,350,489 + -0.8 1,350,587 + flgA MCAODC_23040 0.17 -0.5 1,350,588 - flgA MCAODC_23040 0.18 -1.1 1,350,615 + flgA MCAODC_23040 0.22 -1.5 1,350,615 + flgA MCAODC_23040 0.22 +0.3 1,350,629 + flgA MCAODC_23040 0.24 +0.0 1,350,629 + flgA MCAODC_23040 0.24 +1.0 1,350,630 - flgA MCAODC_23040 0.24 +1.4 1,350,630 - flgA MCAODC_23040 0.24 -0.6 1,350,669 + flgA MCAODC_23040 0.30 +0.9 1,350,669 + flgA MCAODC_23040 0.30 +1.1 1,350,727 + flgA MCAODC_23040 0.39 +0.7 1,350,728 - flgA MCAODC_23040 0.39 +1.4 1,350,731 + flgA MCAODC_23040 0.39 -0.9 1,350,841 - flgA MCAODC_23040 0.56 -0.0 1,350,905 + flgA MCAODC_23040 0.66 +2.5 1,351,082 + -0.0 1,351,083 - +1.6 1,351,120 - +2.1 1,351,122 - -0.2 1,351,165 + +0.5 1,351,165 + -0.4 1,351,179 + -0.0 1,351,202 - -0.0 1,351,212 + -1.3 1,351,212 + -0.6 1,351,241 + flgM MCAODC_23045 0.12 +0.1
Or see this region's nucleotide sequence