Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potB and espK are separated by 363 nucleotides espK and MCAODC_22475 are separated by 188 nucleotides MCAODC_22475 and MCAODC_22480 are separated by 141 nucleotides MCAODC_22480 and tnp-IS3 are separated by 10 nucleotides
MCAODC_22465: potB - spermidine/putrescine ABC transporter permease PotB, at 1,252,410 to 1,253,273
potB
MCAODC_22470: espK - type III secretion system effector EspK, at 1,253,637 to 1,254,998
espK
MCAODC_22475: MCAODC_22475 - hypothetical protein, at 1,255,187 to 1,255,420
_22475
MCAODC_22480: MCAODC_22480 - Mutator family transposase, at 1,255,562 to 1,255,861
_22480
MCAODC_22485: tnp-IS3 - IS3 family transposase, at 1,255,872 to 1,256,033
tnp-IS3
Position (kb) 1253
1254
1255 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1252.941 kb on - strand, within potB at 1252.994 kb on + strand, within potB at 1253.076 kb on + strand, within potB at 1253.076 kb on + strand, within potB at 1253.077 kb on - strand, within potB at 1253.077 kb on - strand, within potB at 1253.077 kb on - strand, within potB at 1253.134 kb on + strand, within potB at 1253.134 kb on + strand, within potB at 1253.147 kb on + strand, within potB at 1253.184 kb on - strand, within potB at 1253.349 kb on - strand at 1253.450 kb on + strand at 1253.762 kb on - strand at 1253.822 kb on + strand, within espK at 1254.021 kb on + strand, within espK at 1254.022 kb on - strand, within espK at 1254.023 kb on + strand, within espK at 1254.024 kb on - strand, within espK at 1254.025 kb on + strand, within espK at 1254.129 kb on + strand, within espK at 1254.130 kb on - strand, within espK at 1254.130 kb on - strand, within espK at 1254.210 kb on - strand, within espK at 1254.212 kb on + strand, within espK at 1254.213 kb on - strand, within espK at 1254.215 kb on - strand, within espK at 1254.503 kb on + strand, within espK at 1254.797 kb on + strand, within espK at 1254.934 kb on + strand at 1255.175 kb on + strand at 1255.185 kb on - strand at 1255.188 kb on + strand at 1255.189 kb on - strand at 1255.189 kb on - strand at 1255.189 kb on - strand at 1255.190 kb on + strand at 1255.295 kb on + strand, within MCAODC_22475 at 1255.302 kb on - strand, within MCAODC_22475 at 1255.332 kb on + strand, within MCAODC_22475 at 1255.416 kb on - strand at 1255.434 kb on - strand at 1255.497 kb on - strand at 1255.510 kb on - strand at 1255.536 kb on - strand at 1255.550 kb on - strand at 1255.825 kb on - strand, within MCAODC_22480 at 1255.831 kb on + strand, within MCAODC_22480 at 1255.831 kb on + strand, within MCAODC_22480
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,252,941 - potB MCAODC_22465 0.61 +1.0 1,252,994 + potB MCAODC_22465 0.68 -0.1 1,253,076 + potB MCAODC_22465 0.77 -0.1 1,253,076 + potB MCAODC_22465 0.77 -0.2 1,253,077 - potB MCAODC_22465 0.77 -0.1 1,253,077 - potB MCAODC_22465 0.77 -0.9 1,253,077 - potB MCAODC_22465 0.77 -0.2 1,253,134 + potB MCAODC_22465 0.84 -1.3 1,253,134 + potB MCAODC_22465 0.84 -1.9 1,253,147 + potB MCAODC_22465 0.85 -0.8 1,253,184 - potB MCAODC_22465 0.90 +0.6 1,253,349 - -0.9 1,253,450 + -0.8 1,253,762 - +1.5 1,253,822 + espK MCAODC_22470 0.14 +0.9 1,254,021 + espK MCAODC_22470 0.28 -0.2 1,254,022 - espK MCAODC_22470 0.28 -0.2 1,254,023 + espK MCAODC_22470 0.28 +0.7 1,254,024 - espK MCAODC_22470 0.28 +0.2 1,254,025 + espK MCAODC_22470 0.28 -1.3 1,254,129 + espK MCAODC_22470 0.36 +0.1 1,254,130 - espK MCAODC_22470 0.36 +3.5 1,254,130 - espK MCAODC_22470 0.36 -1.2 1,254,210 - espK MCAODC_22470 0.42 -0.9 1,254,212 + espK MCAODC_22470 0.42 +0.1 1,254,213 - espK MCAODC_22470 0.42 +1.2 1,254,215 - espK MCAODC_22470 0.42 +1.3 1,254,503 + espK MCAODC_22470 0.64 -0.0 1,254,797 + espK MCAODC_22470 0.85 -0.0 1,254,934 + +0.6 1,255,175 + -0.9 1,255,185 - +1.5 1,255,188 + +0.6 1,255,189 - -1.2 1,255,189 - -0.2 1,255,189 - +1.1 1,255,190 + +0.8 1,255,295 + MCAODC_22475 0.46 -2.5 1,255,302 - MCAODC_22475 0.49 -1.4 1,255,332 + MCAODC_22475 0.62 -0.2 1,255,416 - -1.4 1,255,434 - -1.2 1,255,497 - +0.5 1,255,510 - +1.9 1,255,536 - +1.1 1,255,550 - -1.1 1,255,825 - MCAODC_22480 0.88 +0.9 1,255,831 + MCAODC_22480 0.90 -0.9 1,255,831 + MCAODC_22480 0.90 +0.6
Or see this region's nucleotide sequence