Strain Fitness in Escherichia coli ECRC101 around MCAODC_20480

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpsG and manC2 are separated by 3 nucleotides MCAODC_20480: cpsG - colanic acid biosynthesis phosphomannomutase CpsG, at 903,350 to 904,720 cpsG MCAODC_20485: manC2 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 904,724 to 906,172 manC2 Position (kb) 903 904 905Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 902.417 kb on + strandat 903.119 kb on - strandat 903.316 kb on - strandat 903.721 kb on - strand, within cpsGat 904.235 kb on - strand, within cpsGat 904.235 kb on - strand, within cpsGat 904.237 kb on - strand, within cpsGat 904.721 kb on - strandat 904.760 kb on - strandat 904.760 kb on - strandat 904.760 kb on - strandat 904.780 kb on - strandat 904.861 kb on - strandat 905.072 kb on - strand, within manC2at 905.077 kb on - strand, within manC2at 905.077 kb on - strand, within manC2

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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902,417 + -1.1
903,119 - +2.7
903,316 - +2.3
903,721 - cpsG MCAODC_20480 0.27 +0.4
904,235 - cpsG MCAODC_20480 0.65 -1.2
904,235 - cpsG MCAODC_20480 0.65 -0.5
904,237 - cpsG MCAODC_20480 0.65 -1.1
904,721 - -0.5
904,760 - -0.6
904,760 - -1.6
904,760 - -2.5
904,780 - -4.0
904,861 - +3.0
905,072 - manC2 MCAODC_20485 0.24 -1.5
905,077 - manC2 MCAODC_20485 0.24 -2.6
905,077 - manC2 MCAODC_20485 0.24 -1.5

Or see this region's nucleotide sequence