Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20195 and rfaF are separated by 261 nucleotides rfaF and shiA are separated by 313 nucleotides
MCAODC_20195: MCAODC_20195 - Ig-like domain-containing protein, at 842,684 to 850,693
_20195
MCAODC_20200: rfaF - Glycosyltransferase family 9 (heptosyltransferase), at 850,955 to 852,007
rfaF
MCAODC_20205: shiA - shikimate transporter, at 852,321 to 853,637
shiA
Position (kb) 850
851
852
853 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 849.993 kb on - strand at 850.006 kb on + strand at 850.045 kb on - strand at 850.084 kb on - strand at 850.084 kb on - strand at 850.084 kb on - strand at 850.111 kb on - strand at 850.129 kb on - strand at 850.156 kb on + strand at 850.156 kb on + strand at 850.157 kb on - strand at 850.157 kb on - strand at 850.157 kb on - strand at 850.187 kb on + strand at 850.187 kb on + strand at 850.201 kb on + strand at 850.201 kb on + strand at 850.298 kb on - strand at 850.298 kb on - strand at 850.354 kb on + strand at 850.354 kb on + strand at 850.355 kb on - strand at 850.358 kb on + strand at 850.358 kb on + strand at 850.358 kb on + strand at 850.358 kb on + strand at 850.358 kb on + strand at 850.360 kb on + strand at 850.360 kb on + strand at 850.360 kb on + strand at 850.360 kb on + strand at 850.360 kb on + strand at 850.360 kb on + strand at 850.361 kb on - strand at 850.361 kb on - strand at 850.361 kb on - strand at 850.361 kb on - strand at 850.361 kb on - strand at 850.361 kb on - strand at 850.362 kb on + strand at 850.362 kb on + strand at 850.363 kb on - strand at 850.363 kb on - strand at 850.363 kb on - strand at 850.363 kb on - strand at 850.364 kb on + strand at 850.365 kb on - strand at 850.418 kb on + strand at 850.418 kb on + strand at 850.479 kb on + strand at 850.479 kb on + strand at 850.514 kb on + strand at 850.571 kb on - strand at 850.611 kb on - strand at 850.614 kb on + strand at 850.615 kb on - strand at 850.615 kb on - strand at 850.755 kb on - strand at 850.788 kb on - strand at 850.939 kb on - strand at 850.944 kb on + strand at 850.945 kb on - strand at 850.959 kb on - strand at 851.086 kb on - strand, within rfaF at 851.127 kb on - strand, within rfaF at 851.282 kb on + strand, within rfaF at 851.289 kb on - strand, within rfaF at 851.492 kb on - strand, within rfaF at 851.492 kb on - strand, within rfaF at 851.999 kb on + strand at 852.034 kb on + strand at 852.035 kb on - strand at 852.092 kb on + strand at 852.092 kb on + strand at 852.127 kb on + strand at 852.242 kb on - strand at 852.292 kb on + strand at 852.294 kb on + strand at 852.426 kb on + strand at 852.437 kb on - strand at 852.441 kb on + strand at 852.467 kb on + strand, within shiA at 852.491 kb on - strand, within shiA at 852.600 kb on - strand, within shiA at 852.600 kb on - strand, within shiA at 852.611 kb on - strand, within shiA at 852.657 kb on - strand, within shiA at 852.805 kb on - strand, within shiA at 852.835 kb on + strand, within shiA at 852.933 kb on - strand, within shiA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 849,993 - +0.5 850,006 + +0.7 850,045 - +1.0 850,084 - -1.2 850,084 - -0.0 850,084 - +0.2 850,111 - +1.2 850,129 - +0.7 850,156 + -0.1 850,156 + -0.9 850,157 - +0.5 850,157 - +1.0 850,157 - -1.0 850,187 + -0.2 850,187 + -0.9 850,201 + -1.2 850,201 + +1.4 850,298 - -0.1 850,298 - -1.2 850,354 + -1.2 850,354 + +1.2 850,355 - +1.0 850,358 + +0.2 850,358 + +4.0 850,358 + -0.2 850,358 + -1.0 850,358 + -0.7 850,360 + +1.5 850,360 + -1.5 850,360 + -1.2 850,360 + +0.3 850,360 + +1.0 850,360 + +0.1 850,361 - +0.6 850,361 - +1.2 850,361 - -1.7 850,361 - +2.5 850,361 - -1.3 850,361 - +0.2 850,362 + +0.7 850,362 + +0.2 850,363 - -0.1 850,363 - -1.0 850,363 - +0.9 850,363 - +0.3 850,364 + -1.1 850,365 - +1.0 850,418 + -1.1 850,418 + +3.5 850,479 + +0.2 850,479 + +0.5 850,514 + -2.3 850,571 - -1.3 850,611 - +0.7 850,614 + +0.3 850,615 - +1.3 850,615 - -0.0 850,755 - +0.0 850,788 - +0.7 850,939 - -0.1 850,944 + -1.1 850,945 - -0.3 850,959 - +0.8 851,086 - rfaF MCAODC_20200 0.12 +0.7 851,127 - rfaF MCAODC_20200 0.16 +0.9 851,282 + rfaF MCAODC_20200 0.31 -1.6 851,289 - rfaF MCAODC_20200 0.32 +1.1 851,492 - rfaF MCAODC_20200 0.51 -0.1 851,492 - rfaF MCAODC_20200 0.51 -0.6 851,999 + -2.2 852,034 + +0.6 852,035 - -0.2 852,092 + +1.0 852,092 + -1.1 852,127 + -0.7 852,242 - +0.7 852,292 + +0.0 852,294 + -0.3 852,426 + +0.4 852,437 - +1.7 852,441 + +0.4 852,467 + shiA MCAODC_20205 0.11 +2.1 852,491 - shiA MCAODC_20205 0.13 -0.7 852,600 - shiA MCAODC_20205 0.21 -0.4 852,600 - shiA MCAODC_20205 0.21 -1.6 852,611 - shiA MCAODC_20205 0.22 -0.3 852,657 - shiA MCAODC_20205 0.26 +0.6 852,805 - shiA MCAODC_20205 0.37 -0.1 852,835 + shiA MCAODC_20205 0.39 +0.8 852,933 - shiA MCAODC_20205 0.46 -0.6
Or see this region's nucleotide sequence