Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt espR4 and fliE are separated by 348 nucleotides fliE and fliF are separated by 214 nucleotides fliF and fliG overlap by 8 nucleotides fliG and fliH overlap by 8 nucleotides
MCAODC_19670: espR4 - T3SS effector leucine-rich repeat protein EspR4, at 764,967 to 766,106
espR4
MCAODC_19675: fliE - flagellar hook-basal body complex protein FliE, at 766,455 to 766,769
fliE
MCAODC_19680: fliF - flagellar M-ring protein FliF, at 766,984 to 768,642
fliF
MCAODC_19685: fliG - flagellar motor switch protein FliG, at 768,635 to 769,630
fliG
MCAODC_19690: fliH - flagellar assembly protein FliH, at 769,623 to 770,309
fliH
Position (kb) 766
767
768
769 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 766.327 kb on + strand at 766.328 kb on - strand at 766.330 kb on - strand at 766.437 kb on - strand at 766.437 kb on - strand at 766.456 kb on + strand at 766.456 kb on + strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.474 kb on - strand at 766.474 kb on - strand at 766.500 kb on - strand, within fliE at 766.628 kb on + strand, within fliE at 766.674 kb on - strand, within fliE at 766.674 kb on - strand, within fliE at 766.805 kb on - strand at 766.867 kb on - strand at 766.888 kb on + strand at 767.229 kb on + strand, within fliF at 767.247 kb on + strand, within fliF at 767.439 kb on - strand, within fliF at 767.665 kb on - strand, within fliF at 767.739 kb on + strand, within fliF at 767.740 kb on - strand, within fliF at 767.757 kb on + strand, within fliF at 767.757 kb on + strand, within fliF at 767.758 kb on - strand, within fliF at 767.868 kb on + strand, within fliF at 767.868 kb on + strand, within fliF at 768.016 kb on - strand, within fliF at 768.052 kb on - strand, within fliF at 768.052 kb on - strand, within fliF at 768.055 kb on + strand, within fliF at 768.055 kb on + strand, within fliF at 768.055 kb on + strand, within fliF at 768.056 kb on - strand, within fliF at 768.081 kb on + strand, within fliF at 768.274 kb on - strand, within fliF at 768.335 kb on + strand, within fliF at 768.391 kb on - strand, within fliF at 768.440 kb on + strand, within fliF at 768.440 kb on + strand, within fliF at 768.627 kb on + strand at 768.789 kb on + strand, within fliG at 768.845 kb on - strand, within fliG at 768.860 kb on + strand, within fliG at 768.922 kb on + strand, within fliG at 769.168 kb on - strand, within fliG at 769.240 kb on + strand, within fliG at 769.599 kb on + strand at 769.599 kb on + strand at 769.618 kb on + strand at 769.618 kb on + strand at 769.618 kb on + strand at 769.619 kb on - strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.622 kb on + strand at 769.623 kb on - strand at 769.623 kb on - strand at 769.623 kb on - strand at 769.623 kb on - strand at 769.623 kb on - strand at 769.623 kb on - strand at 769.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 766,327 + +0.8 766,328 - +0.5 766,330 - -0.4 766,437 - +0.6 766,437 - -0.0 766,456 + +3.0 766,456 + +0.3 766,457 - -2.0 766,457 - -0.9 766,457 - -0.8 766,457 - -0.5 766,474 - +0.4 766,474 - -1.0 766,500 - fliE MCAODC_19675 0.14 -1.6 766,628 + fliE MCAODC_19675 0.55 +1.2 766,674 - fliE MCAODC_19675 0.70 -0.1 766,674 - fliE MCAODC_19675 0.70 -0.2 766,805 - +0.2 766,867 - +0.4 766,888 + -0.2 767,229 + fliF MCAODC_19680 0.15 -0.1 767,247 + fliF MCAODC_19680 0.16 -0.9 767,439 - fliF MCAODC_19680 0.27 +3.0 767,665 - fliF MCAODC_19680 0.41 +0.2 767,739 + fliF MCAODC_19680 0.46 -0.4 767,740 - fliF MCAODC_19680 0.46 -0.5 767,757 + fliF MCAODC_19680 0.47 -1.0 767,757 + fliF MCAODC_19680 0.47 +0.3 767,758 - fliF MCAODC_19680 0.47 +0.8 767,868 + fliF MCAODC_19680 0.53 -1.5 767,868 + fliF MCAODC_19680 0.53 +1.2 768,016 - fliF MCAODC_19680 0.62 -2.2 768,052 - fliF MCAODC_19680 0.64 +2.0 768,052 - fliF MCAODC_19680 0.64 +0.4 768,055 + fliF MCAODC_19680 0.65 -2.0 768,055 + fliF MCAODC_19680 0.65 -1.2 768,055 + fliF MCAODC_19680 0.65 -0.5 768,056 - fliF MCAODC_19680 0.65 +1.6 768,081 + fliF MCAODC_19680 0.66 -0.8 768,274 - fliF MCAODC_19680 0.78 +0.4 768,335 + fliF MCAODC_19680 0.81 -0.1 768,391 - fliF MCAODC_19680 0.85 +0.2 768,440 + fliF MCAODC_19680 0.88 -1.0 768,440 + fliF MCAODC_19680 0.88 -1.5 768,627 + -0.4 768,789 + fliG MCAODC_19685 0.15 +0.5 768,845 - fliG MCAODC_19685 0.21 +1.9 768,860 + fliG MCAODC_19685 0.23 +0.7 768,922 + fliG MCAODC_19685 0.29 +0.0 769,168 - fliG MCAODC_19685 0.54 -0.2 769,240 + fliG MCAODC_19685 0.61 +0.3 769,599 + -0.1 769,599 + +0.3 769,618 + -2.7 769,618 + +0.6 769,618 + +0.9 769,619 - -0.0 769,622 + -0.3 769,622 + +1.0 769,622 + -0.5 769,622 + +0.8 769,622 + +0.6 769,622 + -1.4 769,622 + -0.3 769,623 - -0.9 769,623 - +0.8 769,623 - -0.5 769,623 - -0.2 769,623 - -0.6 769,623 - +0.2 769,632 - +0.2
Or see this region's nucleotide sequence