Strain Fitness in Escherichia coli ECRC101 around MCAODC_18735

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeaL and nimR overlap by 44 nucleotidesnimR and nimT are separated by 99 nucleotidesnimT and yeaO are separated by 54 nucleotidesyeaO and yoaF are separated by 21 nucleotidesyoaF and dgcP are separated by 182 nucleotides MCAODC_18725: yeaL - UPF0756 membrane protein CKO_01811, at 605,225 to 605,671 yeaL MCAODC_18730: nimR - DNA-binding transcriptional regulator NimR, at 605,628 to 606,446 nimR MCAODC_18735: nimT - 2-nitroimidazole transporter, at 606,546 to 607,727 nimT MCAODC_18740: yeaO - Uncharacterized protein YeaO, at 607,782 to 608,129 yeaO MCAODC_18745: yoaF - DUF333 domain-containing protein, at 608,151 to 608,405 yoaF MCAODC_18750: dgcP - diguanylate cyclase DgcP, at 608,588 to 609,613 dgcP Position (kb) 606 607 608Strain fitness (log2 ratio) -2 -1 0 1 2 3at 605.630 kb on - strandat 605.670 kb on - strandat 605.670 kb on - strandat 605.684 kb on + strandat 605.685 kb on - strandat 605.724 kb on - strand, within nimRat 605.726 kb on + strand, within nimRat 605.727 kb on - strand, within nimRat 605.727 kb on - strand, within nimRat 605.750 kb on - strand, within nimRat 605.750 kb on - strand, within nimRat 605.976 kb on - strand, within nimRat 606.102 kb on + strand, within nimRat 606.103 kb on - strand, within nimRat 606.143 kb on - strand, within nimRat 606.222 kb on - strand, within nimRat 606.222 kb on - strand, within nimRat 606.222 kb on - strand, within nimRat 606.291 kb on - strand, within nimRat 606.297 kb on - strand, within nimRat 606.468 kb on + strandat 606.469 kb on - strandat 606.497 kb on - strandat 606.517 kb on - strandat 606.546 kb on - strandat 606.546 kb on - strandat 606.617 kb on + strandat 606.618 kb on - strandat 606.704 kb on - strand, within nimTat 606.704 kb on - strand, within nimTat 606.704 kb on - strand, within nimTat 606.786 kb on - strand, within nimTat 606.849 kb on + strand, within nimTat 606.854 kb on - strand, within nimTat 606.895 kb on + strand, within nimTat 606.900 kb on - strand, within nimTat 606.907 kb on + strand, within nimTat 606.923 kb on + strand, within nimTat 606.966 kb on + strand, within nimTat 607.013 kb on - strand, within nimTat 607.151 kb on - strand, within nimTat 607.151 kb on - strand, within nimTat 607.151 kb on - strand, within nimTat 607.151 kb on - strand, within nimTat 607.154 kb on + strand, within nimTat 607.154 kb on + strand, within nimTat 607.225 kb on - strand, within nimTat 607.228 kb on - strand, within nimTat 607.236 kb on - strand, within nimTat 607.315 kb on + strand, within nimTat 607.354 kb on + strand, within nimTat 607.495 kb on + strand, within nimTat 607.495 kb on + strand, within nimTat 607.614 kb on - strandat 607.629 kb on - strandat 607.666 kb on + strandat 607.676 kb on - strandat 607.728 kb on + strandat 607.738 kb on + strandat 607.738 kb on + strandat 607.739 kb on - strandat 607.739 kb on - strandat 607.739 kb on - strandat 607.740 kb on + strandat 607.787 kb on + strandat 607.806 kb on + strandat 607.806 kb on + strandat 607.983 kb on + strand, within yeaOat 607.983 kb on + strand, within yeaOat 608.065 kb on - strand, within yeaOat 608.120 kb on + strandat 608.120 kb on + strandat 608.121 kb on - strandat 608.137 kb on - strandat 608.137 kb on - strandat 608.288 kb on - strand, within yoaFat 608.425 kb on - strandat 608.494 kb on + strandat 608.495 kb on - strandat 608.497 kb on + strandat 608.530 kb on - strandat 608.571 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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605,630 - +0.9
605,670 - +1.3
605,670 - -0.3
605,684 + +0.6
605,685 - -1.6
605,724 - nimR MCAODC_18730 0.12 +0.1
605,726 + nimR MCAODC_18730 0.12 +2.9
605,727 - nimR MCAODC_18730 0.12 +0.6
605,727 - nimR MCAODC_18730 0.12 +1.5
605,750 - nimR MCAODC_18730 0.15 +0.1
605,750 - nimR MCAODC_18730 0.15 -2.2
605,976 - nimR MCAODC_18730 0.42 -0.9
606,102 + nimR MCAODC_18730 0.58 -2.0
606,103 - nimR MCAODC_18730 0.58 +0.4
606,143 - nimR MCAODC_18730 0.63 +0.4
606,222 - nimR MCAODC_18730 0.73 +0.1
606,222 - nimR MCAODC_18730 0.73 -0.5
606,222 - nimR MCAODC_18730 0.73 -1.0
606,291 - nimR MCAODC_18730 0.81 -0.2
606,297 - nimR MCAODC_18730 0.82 -0.8
606,468 + -0.7
606,469 - +0.4
606,497 - +0.4
606,517 - +2.6
606,546 - -0.1
606,546 - -1.0
606,617 + -0.2
606,618 - +1.1
606,704 - nimT MCAODC_18735 0.13 +0.3
606,704 - nimT MCAODC_18735 0.13 -1.9
606,704 - nimT MCAODC_18735 0.13 -1.5
606,786 - nimT MCAODC_18735 0.20 -0.4
606,849 + nimT MCAODC_18735 0.26 +0.7
606,854 - nimT MCAODC_18735 0.26 -0.5
606,895 + nimT MCAODC_18735 0.30 -0.1
606,900 - nimT MCAODC_18735 0.30 +0.2
606,907 + nimT MCAODC_18735 0.31 +0.3
606,923 + nimT MCAODC_18735 0.32 +0.1
606,966 + nimT MCAODC_18735 0.36 -0.4
607,013 - nimT MCAODC_18735 0.40 +0.5
607,151 - nimT MCAODC_18735 0.51 -1.3
607,151 - nimT MCAODC_18735 0.51 +0.8
607,151 - nimT MCAODC_18735 0.51 -1.6
607,151 - nimT MCAODC_18735 0.51 +0.3
607,154 + nimT MCAODC_18735 0.51 +0.9
607,154 + nimT MCAODC_18735 0.51 +1.3
607,225 - nimT MCAODC_18735 0.57 +1.7
607,228 - nimT MCAODC_18735 0.58 -1.1
607,236 - nimT MCAODC_18735 0.58 -0.6
607,315 + nimT MCAODC_18735 0.65 +1.4
607,354 + nimT MCAODC_18735 0.68 +0.5
607,495 + nimT MCAODC_18735 0.80 -1.1
607,495 + nimT MCAODC_18735 0.80 +1.3
607,614 - +1.1
607,629 - -0.8
607,666 + -1.4
607,676 - +0.7
607,728 + -0.8
607,738 + -0.4
607,738 + +0.1
607,739 - -1.7
607,739 - +1.6
607,739 - +0.8
607,740 + -1.6
607,787 + +0.4
607,806 + +0.9
607,806 + -1.9
607,983 + yeaO MCAODC_18740 0.58 -0.4
607,983 + yeaO MCAODC_18740 0.58 -0.7
608,065 - yeaO MCAODC_18740 0.81 +1.3
608,120 + +1.3
608,120 + +0.1
608,121 - -0.3
608,137 - -1.4
608,137 - -1.3
608,288 - yoaF MCAODC_18745 0.54 +1.7
608,425 - +0.6
608,494 + +0.8
608,495 - +0.3
608,497 + +0.1
608,530 - -0.2
608,571 + -1.4

Or see this region's nucleotide sequence