Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lsrB and lsrF are separated by 26 nucleotides lsrF and lsrG are separated by 23 nucleotides lsrG and tam are separated by 56 nucleotides tam and yneE are separated by 3 nucleotides
MCAODC_16770: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 266,547 to 267,569
lsrB
MCAODC_16775: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 267,596 to 268,471
lsrF
MCAODC_16780: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 268,495 to 268,785
lsrG
MCAODC_16785: tam - trans-aconitate 2-methyltransferase, at 268,842 to 269,600
tam
MCAODC_16790: yneE - UPF0187 protein YneE, at 269,604 to 270,518
yneE
Position (kb) 268
269 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 267.577 kb on + strand at 267.811 kb on + strand, within lsrF at 267.812 kb on - strand, within lsrF at 267.817 kb on + strand, within lsrF at 267.846 kb on + strand, within lsrF at 267.846 kb on + strand, within lsrF at 267.850 kb on - strand, within lsrF at 267.949 kb on - strand, within lsrF at 268.023 kb on - strand, within lsrF at 268.061 kb on - strand, within lsrF at 268.151 kb on + strand, within lsrF at 268.212 kb on - strand, within lsrF at 268.442 kb on + strand at 268.451 kb on + strand at 268.469 kb on + strand at 268.469 kb on + strand at 268.583 kb on + strand, within lsrG at 268.583 kb on + strand, within lsrG at 268.660 kb on + strand, within lsrG at 268.662 kb on + strand, within lsrG at 268.864 kb on + strand at 268.865 kb on - strand at 268.942 kb on - strand, within tam at 269.198 kb on - strand, within tam at 269.335 kb on - strand, within tam at 269.391 kb on + strand, within tam at 269.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 267,577 + +0.6 267,811 + lsrF MCAODC_16775 0.25 +2.8 267,812 - lsrF MCAODC_16775 0.25 +1.8 267,817 + lsrF MCAODC_16775 0.25 +0.3 267,846 + lsrF MCAODC_16775 0.29 +0.5 267,846 + lsrF MCAODC_16775 0.29 +0.3 267,850 - lsrF MCAODC_16775 0.29 +0.3 267,949 - lsrF MCAODC_16775 0.40 -0.2 268,023 - lsrF MCAODC_16775 0.49 +0.8 268,061 - lsrF MCAODC_16775 0.53 +0.0 268,151 + lsrF MCAODC_16775 0.63 -0.6 268,212 - lsrF MCAODC_16775 0.70 -0.4 268,442 + -0.7 268,451 + +0.3 268,469 + -0.5 268,469 + +0.9 268,583 + lsrG MCAODC_16780 0.30 -0.7 268,583 + lsrG MCAODC_16780 0.30 +2.8 268,660 + lsrG MCAODC_16780 0.57 -1.0 268,662 + lsrG MCAODC_16780 0.57 -0.4 268,864 + +1.2 268,865 - +0.9 268,942 - tam MCAODC_16785 0.13 -1.3 269,198 - tam MCAODC_16785 0.47 +0.0 269,335 - tam MCAODC_16785 0.65 +1.0 269,391 + tam MCAODC_16785 0.72 -2.3 269,551 + -0.5
Or see this region's nucleotide sequence