Strain Fitness in Escherichia coli ECRC101 around MCAODC_16410

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_16400 and mddA overlap by 4 nucleotidesmddA and curA are separated by 180 nucleotidescurA and mcbR are separated by 197 nucleotidesmcbR and yncD are separated by 35 nucleotides MCAODC_16400: MCAODC_16400 - Putative inner membrane exporter, YdcZ, at 171,209 to 171,658 _16400 MCAODC_16405: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 171,655 to 172,173 mddA MCAODC_16410: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 172,354 to 173,391 curA MCAODC_16415: mcbR - colanic acid/biofilm transcriptional regulator McbR, at 173,589 to 174,254 mcbR MCAODC_16420: yncD - putative TonB-dependent receptor YncD, at 174,290 to 176,392 yncD Position (kb) 172 173 174Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 171.361 kb on + strand, within MCAODC_16400at 171.362 kb on - strand, within MCAODC_16400at 171.435 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.485 kb on - strand, within MCAODC_16400at 171.703 kb on - strandat 172.009 kb on + strand, within mddAat 172.031 kb on - strand, within mddAat 172.102 kb on - strand, within mddAat 172.103 kb on + strand, within mddAat 172.103 kb on + strand, within mddAat 172.192 kb on - strandat 172.192 kb on - strandat 172.192 kb on - strandat 172.212 kb on + strandat 172.212 kb on + strandat 172.318 kb on - strandat 172.479 kb on - strand, within curAat 172.510 kb on + strand, within curAat 172.510 kb on + strand, within curAat 172.511 kb on - strand, within curAat 172.512 kb on + strand, within curAat 172.512 kb on + strand, within curAat 172.512 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.543 kb on + strand, within curAat 172.544 kb on - strand, within curAat 172.673 kb on + strand, within curAat 172.767 kb on + strand, within curAat 172.767 kb on + strand, within curAat 172.767 kb on + strand, within curAat 172.775 kb on + strand, within curAat 172.775 kb on + strand, within curAat 172.775 kb on + strand, within curAat 172.794 kb on + strand, within curAat 172.818 kb on - strand, within curAat 173.013 kb on + strand, within curAat 173.017 kb on + strand, within curAat 173.038 kb on - strand, within curAat 173.070 kb on + strand, within curAat 173.110 kb on + strand, within curAat 173.111 kb on - strand, within curAat 173.113 kb on - strand, within curAat 173.126 kb on + strand, within curAat 173.147 kb on + strand, within curAat 173.163 kb on + strand, within curAat 173.163 kb on + strand, within curAat 173.306 kb on + strandat 173.460 kb on - strandat 173.521 kb on + strandat 173.660 kb on + strand, within mcbRat 173.719 kb on + strand, within mcbRat 173.729 kb on + strand, within mcbRat 173.752 kb on + strand, within mcbRat 173.870 kb on - strand, within mcbRat 174.244 kb on - strandat 174.351 kb on + strandat 174.355 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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171,361 + MCAODC_16400 0.34 +1.8
171,362 - MCAODC_16400 0.34 -0.0
171,435 - MCAODC_16400 0.50 -0.9
171,437 - MCAODC_16400 0.51 +1.2
171,437 - MCAODC_16400 0.51 -0.0
171,437 - MCAODC_16400 0.51 -1.0
171,485 - MCAODC_16400 0.61 -1.4
171,703 - -0.5
172,009 + mddA MCAODC_16405 0.68 +0.4
172,031 - mddA MCAODC_16405 0.72 -0.5
172,102 - mddA MCAODC_16405 0.86 +0.4
172,103 + mddA MCAODC_16405 0.86 +0.1
172,103 + mddA MCAODC_16405 0.86 +0.8
172,192 - +0.5
172,192 - -2.6
172,192 - -0.5
172,212 + +1.4
172,212 + +1.3
172,318 - -0.2
172,479 - curA MCAODC_16410 0.12 -1.9
172,510 + curA MCAODC_16410 0.15 +2.1
172,510 + curA MCAODC_16410 0.15 +1.0
172,511 - curA MCAODC_16410 0.15 -0.4
172,512 + curA MCAODC_16410 0.15 -0.7
172,512 + curA MCAODC_16410 0.15 -0.5
172,512 + curA MCAODC_16410 0.15 -1.9
172,524 + curA MCAODC_16410 0.16 +2.1
172,524 + curA MCAODC_16410 0.16 +2.8
172,524 + curA MCAODC_16410 0.16 +0.1
172,543 + curA MCAODC_16410 0.18 +0.8
172,544 - curA MCAODC_16410 0.18 -1.5
172,673 + curA MCAODC_16410 0.31 +0.0
172,767 + curA MCAODC_16410 0.40 +0.2
172,767 + curA MCAODC_16410 0.40 -0.9
172,767 + curA MCAODC_16410 0.40 -0.5
172,775 + curA MCAODC_16410 0.41 +0.6
172,775 + curA MCAODC_16410 0.41 +3.4
172,775 + curA MCAODC_16410 0.41 +0.7
172,794 + curA MCAODC_16410 0.42 +0.1
172,818 - curA MCAODC_16410 0.45 -0.6
173,013 + curA MCAODC_16410 0.63 +2.5
173,017 + curA MCAODC_16410 0.64 -1.4
173,038 - curA MCAODC_16410 0.66 +1.5
173,070 + curA MCAODC_16410 0.69 -0.1
173,110 + curA MCAODC_16410 0.73 +1.8
173,111 - curA MCAODC_16410 0.73 +0.9
173,113 - curA MCAODC_16410 0.73 +3.4
173,126 + curA MCAODC_16410 0.74 +0.9
173,147 + curA MCAODC_16410 0.76 +0.6
173,163 + curA MCAODC_16410 0.78 -0.5
173,163 + curA MCAODC_16410 0.78 -0.7
173,306 + -0.6
173,460 - -1.1
173,521 + -1.3
173,660 + mcbR MCAODC_16415 0.11 -1.4
173,719 + mcbR MCAODC_16415 0.20 -1.0
173,729 + mcbR MCAODC_16415 0.21 +1.5
173,752 + mcbR MCAODC_16415 0.24 +1.2
173,870 - mcbR MCAODC_16415 0.42 -3.2
174,244 - -0.0
174,351 + -1.2
174,355 - +1.2

Or see this region's nucleotide sequence