Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aldA and gap are separated by 41 nucleotides gap and cybB are separated by 188 nucleotides cybB and ydcA are separated by 244 nucleotides
MCAODC_16235: aldA - aldehyde dehydrogenase, at 143,303 to 144,742
aldA
MCAODC_16240: gap - type I glyceraldehyde-3-phosphate dehydrogenase, at 144,784 to 145,785
gap
MCAODC_16245: cybB - cytochrome b561, at 145,974 to 146,504
cybB
MCAODC_16255: ydcA - Uncharacterized protein YdcA, at 146,749 to 146,922
ydcA
Position (kb) 144
145
146 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 143.815 kb on + strand, within aldA at 144.139 kb on + strand, within aldA at 144.158 kb on - strand, within aldA at 144.194 kb on + strand, within aldA at 144.502 kb on + strand, within aldA at 144.503 kb on - strand, within aldA at 144.548 kb on + strand, within aldA at 144.583 kb on + strand, within aldA at 144.634 kb on + strand at 144.635 kb on - strand at 144.661 kb on + strand at 144.707 kb on + strand at 144.732 kb on + strand at 144.732 kb on + strand at 144.740 kb on + strand at 144.740 kb on + strand at 144.793 kb on - strand at 144.875 kb on - strand at 144.875 kb on - strand at 144.956 kb on - strand, within gap at 145.005 kb on + strand, within gap at 145.162 kb on - strand, within gap at 145.248 kb on + strand, within gap at 145.248 kb on + strand, within gap at 145.251 kb on - strand, within gap at 145.251 kb on - strand, within gap at 145.330 kb on - strand, within gap at 145.330 kb on - strand, within gap at 145.330 kb on - strand, within gap at 145.330 kb on - strand, within gap at 145.333 kb on - strand, within gap at 145.485 kb on + strand, within gap at 145.634 kb on + strand, within gap at 145.659 kb on - strand, within gap at 145.680 kb on + strand, within gap at 145.770 kb on + strand at 145.771 kb on - strand at 145.837 kb on - strand at 146.078 kb on + strand, within cybB at 146.079 kb on - strand, within cybB at 146.187 kb on - strand, within cybB at 146.188 kb on + strand, within cybB at 146.189 kb on - strand, within cybB at 146.192 kb on + strand, within cybB at 146.193 kb on - strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.367 kb on - strand, within cybB at 146.476 kb on + strand at 146.476 kb on + strand at 146.532 kb on - strand at 146.569 kb on - strand at 146.588 kb on + strand at 146.589 kb on - strand at 146.702 kb on + strand at 146.702 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.746 kb on + strand at 146.747 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 143,815 + aldA MCAODC_16235 0.36 +1.8 144,139 + aldA MCAODC_16235 0.58 -0.8 144,158 - aldA MCAODC_16235 0.59 +1.2 144,194 + aldA MCAODC_16235 0.62 +0.5 144,502 + aldA MCAODC_16235 0.83 +1.7 144,503 - aldA MCAODC_16235 0.83 -0.9 144,548 + aldA MCAODC_16235 0.86 +0.2 144,583 + aldA MCAODC_16235 0.89 +0.3 144,634 + +0.5 144,635 - +1.1 144,661 + +2.8 144,707 + -1.5 144,732 + -1.8 144,732 + -2.2 144,740 + +0.7 144,740 + -0.4 144,793 - -0.6 144,875 - -0.1 144,875 - +0.2 144,956 - gap MCAODC_16240 0.17 +0.9 145,005 + gap MCAODC_16240 0.22 +0.1 145,162 - gap MCAODC_16240 0.38 +2.3 145,248 + gap MCAODC_16240 0.46 -0.4 145,248 + gap MCAODC_16240 0.46 +0.4 145,251 - gap MCAODC_16240 0.47 -0.2 145,251 - gap MCAODC_16240 0.47 -1.2 145,330 - gap MCAODC_16240 0.54 +1.9 145,330 - gap MCAODC_16240 0.54 -0.5 145,330 - gap MCAODC_16240 0.54 +0.0 145,330 - gap MCAODC_16240 0.54 -0.8 145,333 - gap MCAODC_16240 0.55 -0.3 145,485 + gap MCAODC_16240 0.70 -0.9 145,634 + gap MCAODC_16240 0.85 -0.2 145,659 - gap MCAODC_16240 0.87 -0.7 145,680 + gap MCAODC_16240 0.89 -0.7 145,770 + +1.9 145,771 - -0.5 145,837 - +1.3 146,078 + cybB MCAODC_16245 0.20 -0.1 146,079 - cybB MCAODC_16245 0.20 -1.9 146,187 - cybB MCAODC_16245 0.40 -2.0 146,188 + cybB MCAODC_16245 0.40 +2.4 146,189 - cybB MCAODC_16245 0.40 +1.6 146,192 + cybB MCAODC_16245 0.41 -0.0 146,193 - cybB MCAODC_16245 0.41 -0.4 146,289 + cybB MCAODC_16245 0.59 +0.8 146,289 + cybB MCAODC_16245 0.59 -1.3 146,289 + cybB MCAODC_16245 0.59 -0.9 146,289 + cybB MCAODC_16245 0.59 +1.2 146,289 + cybB MCAODC_16245 0.59 -0.9 146,289 + cybB MCAODC_16245 0.59 -0.5 146,367 - cybB MCAODC_16245 0.74 -0.8 146,476 + +1.5 146,476 + +0.4 146,532 - +0.2 146,569 - +0.9 146,588 + -0.7 146,589 - +2.0 146,702 + -1.3 146,702 + -2.0 146,722 + +1.9 146,722 + -0.6 146,722 + -0.1 146,746 + +0.3 146,747 - +0.2
Or see this region's nucleotide sequence