Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgpB and lapA are separated by 148 nucleotides lapA and lapB are separated by 6 nucleotides lapB and pyrF are separated by 132 nucleotides pyrF and yciH overlap by 1 nucleotides
MCAODC_15700: pgpB - phosphatidylglycerophosphatase B, at 30,024 to 30,788
pgpB
MCAODC_15705: lapA - lipopolysaccharide assembly protein LapA, at 30,937 to 31,245
lapA
MCAODC_15710: lapB - lipopolysaccharide assembly protein LapB, at 31,252 to 32,421
lapB
MCAODC_15715: pyrF - orotidine-5'-phosphate decarboxylase, at 32,554 to 33,351
pyrF
MCAODC_15720: yciH - stress response translation initiation inhibitor YciH, at 33,351 to 33,677
yciH
Position (kb) 31
32
33 Strain fitness (log2 ratio) -2
-1
0
1 at 30.257 kb on - strand, within pgpB at 30.299 kb on + strand, within pgpB at 30.394 kb on + strand, within pgpB at 30.459 kb on + strand, within pgpB at 30.587 kb on + strand, within pgpB at 30.664 kb on + strand, within pgpB at 30.668 kb on + strand, within pgpB at 30.668 kb on + strand, within pgpB at 30.668 kb on + strand, within pgpB at 30.669 kb on - strand, within pgpB at 30.734 kb on - strand at 30.763 kb on + strand at 30.763 kb on + strand at 30.874 kb on + strand at 30.874 kb on + strand at 30.882 kb on + strand at 30.901 kb on + strand at 30.947 kb on + strand at 31.026 kb on + strand, within lapA at 31.030 kb on + strand, within lapA at 31.056 kb on + strand, within lapA at 31.169 kb on + strand, within lapA at 32.465 kb on - strand at 32.763 kb on + strand, within pyrF at 32.764 kb on - strand, within pyrF at 32.885 kb on + strand, within pyrF at 32.885 kb on + strand, within pyrF at 32.928 kb on + strand, within pyrF at 33.225 kb on + strand, within pyrF at 33.262 kb on + strand, within pyrF at 33.262 kb on + strand, within pyrF at 33.264 kb on + strand, within pyrF at 33.335 kb on + strand at 33.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 30,257 - pgpB MCAODC_15700 0.30 -0.4 30,299 + pgpB MCAODC_15700 0.36 +0.9 30,394 + pgpB MCAODC_15700 0.48 -0.2 30,459 + pgpB MCAODC_15700 0.57 -0.5 30,587 + pgpB MCAODC_15700 0.74 -0.6 30,664 + pgpB MCAODC_15700 0.84 +0.4 30,668 + pgpB MCAODC_15700 0.84 +0.6 30,668 + pgpB MCAODC_15700 0.84 +1.6 30,668 + pgpB MCAODC_15700 0.84 +0.1 30,669 - pgpB MCAODC_15700 0.84 -0.3 30,734 - -1.2 30,763 + +1.7 30,763 + -0.4 30,874 + -1.2 30,874 + -0.7 30,882 + -2.2 30,901 + -1.1 30,947 + +0.1 31,026 + lapA MCAODC_15705 0.29 -0.4 31,030 + lapA MCAODC_15705 0.30 +0.3 31,056 + lapA MCAODC_15705 0.39 -1.9 31,169 + lapA MCAODC_15705 0.75 -0.3 32,465 - -0.3 32,763 + pyrF MCAODC_15715 0.26 +0.5 32,764 - pyrF MCAODC_15715 0.26 -2.4 32,885 + pyrF MCAODC_15715 0.41 -2.3 32,885 + pyrF MCAODC_15715 0.41 -0.5 32,928 + pyrF MCAODC_15715 0.47 +1.1 33,225 + pyrF MCAODC_15715 0.84 +0.4 33,262 + pyrF MCAODC_15715 0.89 +1.5 33,262 + pyrF MCAODC_15715 0.89 +0.8 33,264 + pyrF MCAODC_15715 0.89 -2.0 33,335 + +0.3 33,335 + +1.1
Or see this region's nucleotide sequence