Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_14775 and MCAODC_14780 overlap by 4 nucleotides MCAODC_14780 and MCAODC_14785 are separated by 98 nucleotides MCAODC_14785 and MCAODC_14790 overlap by 4 nucleotides MCAODC_14790 and MCAODC_14795 overlap by 4 nucleotides MCAODC_14795 and MCAODC_14800 overlap by 28 nucleotides MCAODC_14800 and exoD overlap by 1 nucleotides exoD and bet overlap by 4 nucleotides
MCAODC_14775: MCAODC_14775 - Ead/Ea22-like protein, at 2,907,260 to 2,908,207
_14775
MCAODC_14780: MCAODC_14780 - conjugal transfer protein TraR, at 2,908,204 to 2,908,425
_14780
MCAODC_14785: MCAODC_14785 - Cell division protein ZapA, at 2,908,524 to 2,908,739
_14785
MCAODC_14790: MCAODC_14790 - DUF1382 domain-containing protein, at 2,908,736 to 2,908,819
_14790
MCAODC_14795: MCAODC_14795 - DUF1382 domain-containing protein, at 2,908,816 to 2,909,007
_14795
MCAODC_14800: MCAODC_14800 - cruciferin, at 2,908,980 to 2,909,162
_14800
MCAODC_14805: exoD - Putative uncharacterized protein ExoD, at 2,909,162 to 2,909,839
exoD
MCAODC_14810: bet - phage recombination protein Bet, at 2,909,836 to 2,910,621
bet
Position (kb)
2908
2909
2910 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2908.133 kb on - strand at 2908.133 kb on - strand at 2908.133 kb on - strand at 2908.133 kb on - strand at 2908.133 kb on - strand at 2908.301 kb on - strand, within MCAODC_14780 at 2908.385 kb on - strand, within MCAODC_14780 at 2908.496 kb on - strand at 2908.765 kb on + strand, within MCAODC_14790 at 2908.765 kb on + strand, within MCAODC_14790 at 2908.765 kb on + strand, within MCAODC_14790 at 2908.956 kb on + strand, within MCAODC_14795 at 2909.007 kb on + strand at 2909.007 kb on + strand at 2909.028 kb on + strand, within MCAODC_14800 at 2909.142 kb on - strand, within MCAODC_14800 at 2909.142 kb on - strand, within MCAODC_14800 at 2909.179 kb on - strand at 2909.181 kb on - strand at 2909.181 kb on - strand at 2909.283 kb on + strand, within exoD at 2909.283 kb on + strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.284 kb on - strand, within exoD at 2909.316 kb on + strand, within exoD at 2909.328 kb on + strand, within exoD at 2909.328 kb on + strand, within exoD at 2909.589 kb on + strand, within exoD at 2909.590 kb on - strand, within exoD at 2909.615 kb on - strand, within exoD at 2909.720 kb on + strand, within exoD at 2909.720 kb on + strand, within exoD at 2909.721 kb on - strand, within exoD at 2909.721 kb on - strand, within exoD at 2909.721 kb on - strand, within exoD at 2909.962 kb on + strand, within bet at 2909.962 kb on + strand, within bet
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 2,908,133 - -1.2 2,908,133 - -1.4 2,908,133 - -1.4 2,908,133 - -1.9 2,908,133 - -2.6 2,908,301 - MCAODC_14780 0.44 +0.5 2,908,385 - MCAODC_14780 0.82 -1.2 2,908,496 - -0.3 2,908,765 + MCAODC_14790 0.35 -0.7 2,908,765 + MCAODC_14790 0.35 +2.3 2,908,765 + MCAODC_14790 0.35 -2.0 2,908,956 + MCAODC_14795 0.73 +0.8 2,909,007 + -0.0 2,909,007 + +0.3 2,909,028 + MCAODC_14800 0.26 +0.2 2,909,142 - MCAODC_14800 0.89 -1.7 2,909,142 - MCAODC_14800 0.89 -0.9 2,909,179 - +0.9 2,909,181 - -0.5 2,909,181 - -1.0 2,909,283 + exoD MCAODC_14805 0.18 -0.9 2,909,283 + exoD MCAODC_14805 0.18 -0.9 2,909,284 - exoD MCAODC_14805 0.18 +0.1 2,909,284 - exoD MCAODC_14805 0.18 +0.5 2,909,284 - exoD MCAODC_14805 0.18 -0.1 2,909,284 - exoD MCAODC_14805 0.18 -0.6 2,909,284 - exoD MCAODC_14805 0.18 +0.0 2,909,284 - exoD MCAODC_14805 0.18 +0.8 2,909,316 + exoD MCAODC_14805 0.23 +1.0 2,909,328 + exoD MCAODC_14805 0.24 -3.2 2,909,328 + exoD MCAODC_14805 0.24 -0.7 2,909,589 + exoD MCAODC_14805 0.63 +2.3 2,909,590 - exoD MCAODC_14805 0.63 +0.4 2,909,615 - exoD MCAODC_14805 0.67 +0.2 2,909,720 + exoD MCAODC_14805 0.82 +1.3 2,909,720 + exoD MCAODC_14805 0.82 +1.7 2,909,721 - exoD MCAODC_14805 0.82 +0.5 2,909,721 - exoD MCAODC_14805 0.82 -1.3 2,909,721 - exoD MCAODC_14805 0.82 -0.7 2,909,962 + bet MCAODC_14810 0.16 -1.2 2,909,962 + bet MCAODC_14810 0.16 -0.9
Or see this region's nucleotide sequence