Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaQ and rfaG overlap by 4 nucleotides rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides
MCAODC_06160: rfaQ - lipopolysaccharide core heptosyltransferase RfaQ, at 1,234,185 to 1,235,243
rfaQ
MCAODC_06165: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,235,240 to 1,236,364
rfaG
MCAODC_06170: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,236,357 to 1,237,154
rfaP
MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204
waaO
Position (kb) 1235
1236
1237 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1234.246 kb on + strand at 1234.246 kb on + strand at 1234.262 kb on + strand at 1234.263 kb on - strand at 1234.370 kb on - strand, within rfaQ at 1234.430 kb on - strand, within rfaQ at 1234.432 kb on + strand, within rfaQ at 1234.432 kb on + strand, within rfaQ at 1234.433 kb on - strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.487 kb on + strand, within rfaQ at 1234.488 kb on - strand, within rfaQ at 1234.489 kb on + strand, within rfaQ at 1234.489 kb on + strand, within rfaQ at 1234.533 kb on + strand, within rfaQ at 1234.534 kb on - strand, within rfaQ at 1234.613 kb on + strand, within rfaQ at 1234.619 kb on - strand, within rfaQ at 1234.697 kb on + strand, within rfaQ at 1234.697 kb on + strand, within rfaQ at 1234.698 kb on - strand, within rfaQ at 1234.737 kb on + strand, within rfaQ at 1234.754 kb on + strand, within rfaQ at 1234.816 kb on + strand, within rfaQ at 1234.816 kb on + strand, within rfaQ at 1234.816 kb on + strand, within rfaQ at 1234.816 kb on + strand, within rfaQ at 1234.816 kb on + strand, within rfaQ at 1234.817 kb on - strand, within rfaQ at 1234.817 kb on - strand, within rfaQ at 1234.817 kb on - strand, within rfaQ at 1234.817 kb on - strand, within rfaQ at 1234.817 kb on - strand, within rfaQ at 1235.009 kb on + strand, within rfaQ at 1235.028 kb on - strand, within rfaQ at 1235.087 kb on + strand, within rfaQ at 1235.088 kb on - strand, within rfaQ at 1235.160 kb on + strand at 1235.160 kb on + strand at 1235.160 kb on + strand at 1235.160 kb on + strand at 1235.231 kb on + strand at 1235.231 kb on + strand at 1235.239 kb on + strand at 1235.240 kb on - strand at 1235.300 kb on - strand at 1235.382 kb on + strand, within rfaG at 1235.416 kb on + strand, within rfaG at 1235.546 kb on + strand, within rfaG at 1235.547 kb on - strand, within rfaG at 1235.610 kb on - strand, within rfaG at 1235.610 kb on - strand, within rfaG at 1235.703 kb on - strand, within rfaG at 1235.728 kb on + strand, within rfaG at 1235.762 kb on + strand, within rfaG at 1235.763 kb on - strand, within rfaG at 1235.931 kb on + strand, within rfaG at 1235.932 kb on - strand, within rfaG at 1235.932 kb on - strand, within rfaG at 1235.932 kb on - strand, within rfaG at 1235.933 kb on + strand, within rfaG at 1235.934 kb on - strand, within rfaG at 1235.951 kb on - strand, within rfaG at 1236.057 kb on + strand, within rfaG at 1236.057 kb on + strand, within rfaG at 1236.132 kb on + strand, within rfaG at 1236.132 kb on + strand, within rfaG at 1236.161 kb on + strand, within rfaG at 1236.161 kb on + strand, within rfaG at 1236.163 kb on + strand, within rfaG at 1236.231 kb on + strand, within rfaG at 1236.309 kb on + strand at 1236.311 kb on + strand at 1236.312 kb on - strand at 1236.343 kb on - strand at 1236.368 kb on + strand at 1236.495 kb on + strand, within rfaP at 1236.568 kb on + strand, within rfaP at 1236.629 kb on + strand, within rfaP at 1236.629 kb on + strand, within rfaP at 1236.686 kb on + strand, within rfaP at 1236.686 kb on + strand, within rfaP at 1236.687 kb on - strand, within rfaP at 1236.715 kb on + strand, within rfaP at 1236.782 kb on + strand, within rfaP at 1236.786 kb on - strand, within rfaP at 1236.846 kb on + strand, within rfaP at 1236.847 kb on - strand, within rfaP at 1236.847 kb on - strand, within rfaP at 1236.848 kb on + strand, within rfaP at 1236.849 kb on - strand, within rfaP at 1237.044 kb on + strand, within rfaP at 1237.152 kb on + strand at 1237.282 kb on + strand at 1237.324 kb on + strand, within waaO
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,234,246 + +1.4 1,234,246 + +1.9 1,234,262 + +1.2 1,234,263 - -0.3 1,234,370 - rfaQ MCAODC_06160 0.17 -0.8 1,234,430 - rfaQ MCAODC_06160 0.23 +1.0 1,234,432 + rfaQ MCAODC_06160 0.23 +0.8 1,234,432 + rfaQ MCAODC_06160 0.23 +2.3 1,234,433 - rfaQ MCAODC_06160 0.23 -2.7 1,234,487 + rfaQ MCAODC_06160 0.29 +1.7 1,234,487 + rfaQ MCAODC_06160 0.29 +1.7 1,234,487 + rfaQ MCAODC_06160 0.29 +2.1 1,234,487 + rfaQ MCAODC_06160 0.29 -0.2 1,234,487 + rfaQ MCAODC_06160 0.29 +1.8 1,234,487 + rfaQ MCAODC_06160 0.29 +1.4 1,234,487 + rfaQ MCAODC_06160 0.29 +1.7 1,234,487 + rfaQ MCAODC_06160 0.29 +2.4 1,234,488 - rfaQ MCAODC_06160 0.29 -2.7 1,234,489 + rfaQ MCAODC_06160 0.29 +1.3 1,234,489 + rfaQ MCAODC_06160 0.29 +1.4 1,234,533 + rfaQ MCAODC_06160 0.33 +1.2 1,234,534 - rfaQ MCAODC_06160 0.33 -2.8 1,234,613 + rfaQ MCAODC_06160 0.40 +1.1 1,234,619 - rfaQ MCAODC_06160 0.41 -0.7 1,234,697 + rfaQ MCAODC_06160 0.48 +0.8 1,234,697 + rfaQ MCAODC_06160 0.48 +0.4 1,234,698 - rfaQ MCAODC_06160 0.48 -1.7 1,234,737 + rfaQ MCAODC_06160 0.52 +1.8 1,234,754 + rfaQ MCAODC_06160 0.54 +1.7 1,234,816 + rfaQ MCAODC_06160 0.60 +1.6 1,234,816 + rfaQ MCAODC_06160 0.60 +1.9 1,234,816 + rfaQ MCAODC_06160 0.60 +1.3 1,234,816 + rfaQ MCAODC_06160 0.60 +1.5 1,234,816 + rfaQ MCAODC_06160 0.60 +2.0 1,234,817 - rfaQ MCAODC_06160 0.60 -1.5 1,234,817 - rfaQ MCAODC_06160 0.60 -0.9 1,234,817 - rfaQ MCAODC_06160 0.60 -2.4 1,234,817 - rfaQ MCAODC_06160 0.60 -0.4 1,234,817 - rfaQ MCAODC_06160 0.60 -1.4 1,235,009 + rfaQ MCAODC_06160 0.78 +2.4 1,235,028 - rfaQ MCAODC_06160 0.80 +1.8 1,235,087 + rfaQ MCAODC_06160 0.85 +3.0 1,235,088 - rfaQ MCAODC_06160 0.85 -1.1 1,235,160 + +2.1 1,235,160 + +1.5 1,235,160 + +2.6 1,235,160 + +2.2 1,235,231 + -2.0 1,235,231 + +0.6 1,235,239 + +0.2 1,235,240 - -3.3 1,235,300 - -0.4 1,235,382 + rfaG MCAODC_06165 0.13 -0.1 1,235,416 + rfaG MCAODC_06165 0.16 -1.1 1,235,546 + rfaG MCAODC_06165 0.27 -1.5 1,235,547 - rfaG MCAODC_06165 0.27 +0.2 1,235,610 - rfaG MCAODC_06165 0.33 -1.6 1,235,610 - rfaG MCAODC_06165 0.33 -1.0 1,235,703 - rfaG MCAODC_06165 0.41 -2.3 1,235,728 + rfaG MCAODC_06165 0.43 -2.2 1,235,762 + rfaG MCAODC_06165 0.46 -2.9 1,235,763 - rfaG MCAODC_06165 0.46 -2.2 1,235,931 + rfaG MCAODC_06165 0.61 +0.0 1,235,932 - rfaG MCAODC_06165 0.62 +0.2 1,235,932 - rfaG MCAODC_06165 0.62 -0.1 1,235,932 - rfaG MCAODC_06165 0.62 -1.1 1,235,933 + rfaG MCAODC_06165 0.62 -3.9 1,235,934 - rfaG MCAODC_06165 0.62 -0.1 1,235,951 - rfaG MCAODC_06165 0.63 +1.4 1,236,057 + rfaG MCAODC_06165 0.73 -0.4 1,236,057 + rfaG MCAODC_06165 0.73 +0.6 1,236,132 + rfaG MCAODC_06165 0.79 -0.2 1,236,132 + rfaG MCAODC_06165 0.79 -1.8 1,236,161 + rfaG MCAODC_06165 0.82 +0.6 1,236,161 + rfaG MCAODC_06165 0.82 -2.3 1,236,163 + rfaG MCAODC_06165 0.82 -1.9 1,236,231 + rfaG MCAODC_06165 0.88 +0.6 1,236,309 + -1.3 1,236,311 + -2.6 1,236,312 - +0.2 1,236,343 - +0.4 1,236,368 + -1.5 1,236,495 + rfaP MCAODC_06170 0.17 -1.5 1,236,568 + rfaP MCAODC_06170 0.26 -1.2 1,236,629 + rfaP MCAODC_06170 0.34 -1.4 1,236,629 + rfaP MCAODC_06170 0.34 -1.2 1,236,686 + rfaP MCAODC_06170 0.41 -1.2 1,236,686 + rfaP MCAODC_06170 0.41 -3.4 1,236,687 - rfaP MCAODC_06170 0.41 +0.6 1,236,715 + rfaP MCAODC_06170 0.45 -0.6 1,236,782 + rfaP MCAODC_06170 0.53 -1.4 1,236,786 - rfaP MCAODC_06170 0.54 -0.3 1,236,846 + rfaP MCAODC_06170 0.61 -1.2 1,236,847 - rfaP MCAODC_06170 0.61 -0.3 1,236,847 - rfaP MCAODC_06170 0.61 -0.6 1,236,848 + rfaP MCAODC_06170 0.62 -2.4 1,236,849 - rfaP MCAODC_06170 0.62 -0.4 1,237,044 + rfaP MCAODC_06170 0.86 +0.4 1,237,152 + -1.6 1,237,282 + -1.0 1,237,324 + waaO MCAODC_06175 0.13 +0.6
Or see this region's nucleotide sequence