Strain Fitness in Escherichia coli ECRC101 around MCAODC_05040

Experiment: WM_EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzyE and rffT overlap by 4 nucleotidesrffT and wzxE overlap by 4 nucleotides MCAODC_05035: wzyE - ECA oligosaccharide polymerase, at 1,012,822 to 1,014,174 wzyE MCAODC_05040: rffT - TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase, at 1,014,171 to 1,015,250 rffT MCAODC_05045: wzxE - lipid III flippase WzxE, at 1,015,247 to 1,016,497 wzxE Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1014.219 kb on + strandat 1014.219 kb on + strandat 1014.219 kb on + strandat 1014.219 kb on + strandat 1014.219 kb on + strandat 1014.219 kb on + strandat 1014.220 kb on - strandat 1014.220 kb on - strandat 1014.223 kb on + strandat 1014.224 kb on - strandat 1014.227 kb on + strandat 1014.227 kb on + strandat 1014.228 kb on - strandat 1014.228 kb on - strandat 1014.234 kb on - strandat 1014.254 kb on + strandat 1014.317 kb on + strand, within rffTat 1014.318 kb on - strand, within rffTat 1014.374 kb on + strand, within rffTat 1014.568 kb on + strand, within rffTat 1014.568 kb on + strand, within rffTat 1014.568 kb on + strand, within rffTat 1014.568 kb on + strand, within rffTat 1014.569 kb on - strand, within rffTat 1014.569 kb on - strand, within rffTat 1014.569 kb on - strand, within rffTat 1014.569 kb on - strand, within rffTat 1014.579 kb on - strand, within rffTat 1014.612 kb on + strand, within rffTat 1014.613 kb on - strand, within rffTat 1014.656 kb on + strand, within rffTat 1014.656 kb on + strand, within rffTat 1014.656 kb on + strand, within rffTat 1014.656 kb on + strand, within rffTat 1014.766 kb on + strand, within rffTat 1014.767 kb on - strand, within rffTat 1014.835 kb on - strand, within rffTat 1014.835 kb on - strand, within rffTat 1014.835 kb on - strand, within rffTat 1014.835 kb on - strand, within rffTat 1014.887 kb on - strand, within rffTat 1014.929 kb on + strand, within rffTat 1014.930 kb on - strand, within rffTat 1014.953 kb on + strand, within rffTat 1014.953 kb on + strand, within rffTat 1014.953 kb on + strand, within rffTat 1014.953 kb on + strand, within rffTat 1014.954 kb on - strand, within rffTat 1015.106 kb on + strand, within rffTat 1015.106 kb on + strand, within rffTat 1015.107 kb on - strand, within rffTat 1015.107 kb on - strand, within rffTat 1015.114 kb on - strand, within rffTat 1015.114 kb on - strand, within rffTat 1015.114 kb on - strand, within rffTat 1015.114 kb on - strand, within rffTat 1015.114 kb on - strand, within rffTat 1015.242 kb on + strandat 1015.242 kb on + strandat 1015.259 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV240
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1,014,219 + +0.3
1,014,219 + -2.5
1,014,219 + -1.1
1,014,219 + +0.1
1,014,219 + -2.8
1,014,219 + -2.2
1,014,220 - -1.6
1,014,220 - -2.9
1,014,223 + -2.2
1,014,224 - -3.9
1,014,227 + -2.5
1,014,227 + -1.7
1,014,228 - -3.3
1,014,228 - -1.0
1,014,234 - -3.2
1,014,254 + -1.7
1,014,317 + rffT MCAODC_05040 0.14 -2.8
1,014,318 - rffT MCAODC_05040 0.14 +0.3
1,014,374 + rffT MCAODC_05040 0.19 -0.4
1,014,568 + rffT MCAODC_05040 0.37 -0.2
1,014,568 + rffT MCAODC_05040 0.37 -0.8
1,014,568 + rffT MCAODC_05040 0.37 -2.6
1,014,568 + rffT MCAODC_05040 0.37 -0.5
1,014,569 - rffT MCAODC_05040 0.37 -3.4
1,014,569 - rffT MCAODC_05040 0.37 -2.8
1,014,569 - rffT MCAODC_05040 0.37 -2.8
1,014,569 - rffT MCAODC_05040 0.37 -1.7
1,014,579 - rffT MCAODC_05040 0.38 -3.7
1,014,612 + rffT MCAODC_05040 0.41 -0.7
1,014,613 - rffT MCAODC_05040 0.41 -1.9
1,014,656 + rffT MCAODC_05040 0.45 -2.3
1,014,656 + rffT MCAODC_05040 0.45 -2.2
1,014,656 + rffT MCAODC_05040 0.45 -3.1
1,014,656 + rffT MCAODC_05040 0.45 -2.7
1,014,766 + rffT MCAODC_05040 0.55 +0.9
1,014,767 - rffT MCAODC_05040 0.55 -2.4
1,014,835 - rffT MCAODC_05040 0.61 -2.8
1,014,835 - rffT MCAODC_05040 0.61 -4.1
1,014,835 - rffT MCAODC_05040 0.61 -4.0
1,014,835 - rffT MCAODC_05040 0.61 -2.3
1,014,887 - rffT MCAODC_05040 0.66 -2.8
1,014,929 + rffT MCAODC_05040 0.70 -1.1
1,014,930 - rffT MCAODC_05040 0.70 -2.1
1,014,953 + rffT MCAODC_05040 0.72 -1.3
1,014,953 + rffT MCAODC_05040 0.72 -1.9
1,014,953 + rffT MCAODC_05040 0.72 -0.3
1,014,953 + rffT MCAODC_05040 0.72 -0.4
1,014,954 - rffT MCAODC_05040 0.72 -3.2
1,015,106 + rffT MCAODC_05040 0.87 -0.5
1,015,106 + rffT MCAODC_05040 0.87 -1.0
1,015,107 - rffT MCAODC_05040 0.87 -3.3
1,015,107 - rffT MCAODC_05040 0.87 -0.8
1,015,114 - rffT MCAODC_05040 0.87 -4.5
1,015,114 - rffT MCAODC_05040 0.87 -1.4
1,015,114 - rffT MCAODC_05040 0.87 -0.7
1,015,114 - rffT MCAODC_05040 0.87 -1.5
1,015,114 - rffT MCAODC_05040 0.87 -3.3
1,015,242 + -5.5
1,015,242 + -1.0
1,015,259 - -0.7

Or see this region's nucleotide sequence