Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzyE and rffT overlap by 4 nucleotides rffT and wzxE overlap by 4 nucleotides
MCAODC_05035: wzyE - ECA oligosaccharide polymerase, at 1,012,822 to 1,014,174
wzyE
MCAODC_05040: rffT - TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase, at 1,014,171 to 1,015,250
rffT
MCAODC_05045: wzxE - lipid III flippase WzxE, at 1,015,247 to 1,016,497
wzxE
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1014.219 kb on + strand at 1014.219 kb on + strand at 1014.219 kb on + strand at 1014.219 kb on + strand at 1014.219 kb on + strand at 1014.219 kb on + strand at 1014.220 kb on - strand at 1014.220 kb on - strand at 1014.223 kb on + strand at 1014.224 kb on - strand at 1014.227 kb on + strand at 1014.227 kb on + strand at 1014.228 kb on - strand at 1014.228 kb on - strand at 1014.234 kb on - strand at 1014.254 kb on + strand at 1014.317 kb on + strand, within rffT at 1014.318 kb on - strand, within rffT at 1014.374 kb on + strand, within rffT at 1014.568 kb on + strand, within rffT at 1014.568 kb on + strand, within rffT at 1014.568 kb on + strand, within rffT at 1014.568 kb on + strand, within rffT at 1014.569 kb on - strand, within rffT at 1014.569 kb on - strand, within rffT at 1014.569 kb on - strand, within rffT at 1014.569 kb on - strand, within rffT at 1014.579 kb on - strand, within rffT at 1014.612 kb on + strand, within rffT at 1014.613 kb on - strand, within rffT at 1014.656 kb on + strand, within rffT at 1014.656 kb on + strand, within rffT at 1014.656 kb on + strand, within rffT at 1014.656 kb on + strand, within rffT at 1014.766 kb on + strand, within rffT at 1014.767 kb on - strand, within rffT at 1014.835 kb on - strand, within rffT at 1014.835 kb on - strand, within rffT at 1014.835 kb on - strand, within rffT at 1014.835 kb on - strand, within rffT at 1014.887 kb on - strand, within rffT at 1014.929 kb on + strand, within rffT at 1014.930 kb on - strand, within rffT at 1014.953 kb on + strand, within rffT at 1014.953 kb on + strand, within rffT at 1014.953 kb on + strand, within rffT at 1014.953 kb on + strand, within rffT at 1014.954 kb on - strand, within rffT at 1015.106 kb on + strand, within rffT at 1015.106 kb on + strand, within rffT at 1015.107 kb on - strand, within rffT at 1015.107 kb on - strand, within rffT at 1015.114 kb on - strand, within rffT at 1015.114 kb on - strand, within rffT at 1015.114 kb on - strand, within rffT at 1015.114 kb on - strand, within rffT at 1015.114 kb on - strand, within rffT at 1015.242 kb on + strand at 1015.242 kb on + strand at 1015.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 1,014,219 + +0.3 1,014,219 + -2.5 1,014,219 + -1.1 1,014,219 + +0.1 1,014,219 + -2.8 1,014,219 + -2.2 1,014,220 - -1.6 1,014,220 - -2.9 1,014,223 + -2.2 1,014,224 - -3.9 1,014,227 + -2.5 1,014,227 + -1.7 1,014,228 - -3.3 1,014,228 - -1.0 1,014,234 - -3.2 1,014,254 + -1.7 1,014,317 + rffT MCAODC_05040 0.14 -2.8 1,014,318 - rffT MCAODC_05040 0.14 +0.3 1,014,374 + rffT MCAODC_05040 0.19 -0.4 1,014,568 + rffT MCAODC_05040 0.37 -0.2 1,014,568 + rffT MCAODC_05040 0.37 -0.8 1,014,568 + rffT MCAODC_05040 0.37 -2.6 1,014,568 + rffT MCAODC_05040 0.37 -0.5 1,014,569 - rffT MCAODC_05040 0.37 -3.4 1,014,569 - rffT MCAODC_05040 0.37 -2.8 1,014,569 - rffT MCAODC_05040 0.37 -2.8 1,014,569 - rffT MCAODC_05040 0.37 -1.7 1,014,579 - rffT MCAODC_05040 0.38 -3.7 1,014,612 + rffT MCAODC_05040 0.41 -0.7 1,014,613 - rffT MCAODC_05040 0.41 -1.9 1,014,656 + rffT MCAODC_05040 0.45 -2.3 1,014,656 + rffT MCAODC_05040 0.45 -2.2 1,014,656 + rffT MCAODC_05040 0.45 -3.1 1,014,656 + rffT MCAODC_05040 0.45 -2.7 1,014,766 + rffT MCAODC_05040 0.55 +0.9 1,014,767 - rffT MCAODC_05040 0.55 -2.4 1,014,835 - rffT MCAODC_05040 0.61 -2.8 1,014,835 - rffT MCAODC_05040 0.61 -4.1 1,014,835 - rffT MCAODC_05040 0.61 -4.0 1,014,835 - rffT MCAODC_05040 0.61 -2.3 1,014,887 - rffT MCAODC_05040 0.66 -2.8 1,014,929 + rffT MCAODC_05040 0.70 -1.1 1,014,930 - rffT MCAODC_05040 0.70 -2.1 1,014,953 + rffT MCAODC_05040 0.72 -1.3 1,014,953 + rffT MCAODC_05040 0.72 -1.9 1,014,953 + rffT MCAODC_05040 0.72 -0.3 1,014,953 + rffT MCAODC_05040 0.72 -0.4 1,014,954 - rffT MCAODC_05040 0.72 -3.2 1,015,106 + rffT MCAODC_05040 0.87 -0.5 1,015,106 + rffT MCAODC_05040 0.87 -1.0 1,015,107 - rffT MCAODC_05040 0.87 -3.3 1,015,107 - rffT MCAODC_05040 0.87 -0.8 1,015,114 - rffT MCAODC_05040 0.87 -4.5 1,015,114 - rffT MCAODC_05040 0.87 -1.4 1,015,114 - rffT MCAODC_05040 0.87 -0.7 1,015,114 - rffT MCAODC_05040 0.87 -1.5 1,015,114 - rffT MCAODC_05040 0.87 -3.3 1,015,242 + -5.5 1,015,242 + -1.0 1,015,259 - -0.7
Or see this region's nucleotide sequence