Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflX and hfq are separated by 76 nucleotides hfq and miaA are separated by 85 nucleotides miaA and mutL overlap by 8 nucleotides
MCAODC_02765: hflX - GTPase HflX, at 558,387 to 559,667
hflX
MCAODC_02770: hfq - RNA chaperone Hfq, at 559,744 to 560,052
hfq
MCAODC_02775: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 560,138 to 561,088
miaA
MCAODC_02780: mutL - DNA mismatch repair endonuclease MutL, at 561,081 to 562,928
mutL
Position (kb) 560
561
562 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 559.147 kb on - strand, within hflX at 559.147 kb on - strand, within hflX at 559.308 kb on - strand, within hflX at 559.341 kb on - strand, within hflX at 559.341 kb on - strand, within hflX at 559.485 kb on - strand, within hflX at 559.495 kb on - strand, within hflX at 559.495 kb on - strand, within hflX at 559.614 kb on - strand at 559.619 kb on - strand at 559.619 kb on - strand at 559.619 kb on - strand at 559.671 kb on + strand at 559.672 kb on - strand at 559.672 kb on - strand at 559.672 kb on - strand at 559.672 kb on - strand at 559.672 kb on - strand at 559.672 kb on - strand at 559.674 kb on - strand at 559.674 kb on - strand at 559.685 kb on + strand at 559.686 kb on - strand at 559.686 kb on - strand at 559.686 kb on - strand at 559.686 kb on - strand at 559.795 kb on - strand, within hfq at 559.795 kb on - strand, within hfq at 559.805 kb on + strand, within hfq at 559.806 kb on - strand, within hfq at 559.806 kb on - strand, within hfq at 559.810 kb on - strand, within hfq at 559.810 kb on - strand, within hfq at 559.810 kb on - strand, within hfq at 559.837 kb on - strand, within hfq at 560.075 kb on - strand at 560.075 kb on - strand at 560.075 kb on - strand at 560.081 kb on - strand at 560.119 kb on - strand at 560.119 kb on - strand at 560.132 kb on - strand at 560.132 kb on - strand at 560.132 kb on - strand at 560.166 kb on - strand at 560.169 kb on - strand at 560.169 kb on - strand at 560.169 kb on - strand at 560.299 kb on - strand, within miaA at 560.320 kb on - strand, within miaA at 560.320 kb on - strand, within miaA at 560.369 kb on - strand, within miaA at 560.511 kb on - strand, within miaA at 560.758 kb on - strand, within miaA at 560.768 kb on - strand, within miaA at 560.904 kb on - strand, within miaA at 560.953 kb on + strand, within miaA at 561.058 kb on + strand at 561.059 kb on - strand at 561.074 kb on - strand at 561.080 kb on - strand at 561.115 kb on - strand at 561.115 kb on - strand at 561.115 kb on - strand at 561.169 kb on - strand at 561.261 kb on - strand at 561.291 kb on + strand, within mutL at 561.295 kb on + strand, within mutL at 561.295 kb on + strand, within mutL at 561.296 kb on - strand, within mutL at 561.296 kb on - strand, within mutL at 561.296 kb on - strand, within mutL at 561.296 kb on - strand, within mutL at 561.300 kb on + strand, within mutL at 561.300 kb on + strand, within mutL at 561.300 kb on + strand, within mutL at 561.301 kb on - strand, within mutL at 561.301 kb on - strand, within mutL at 561.301 kb on - strand, within mutL at 561.301 kb on - strand, within mutL at 561.301 kb on - strand, within mutL at 561.301 kb on - strand, within mutL at 561.345 kb on + strand, within mutL at 561.556 kb on - strand, within mutL at 561.780 kb on - strand, within mutL at 561.780 kb on - strand, within mutL at 561.780 kb on - strand, within mutL at 561.802 kb on + strand, within mutL at 561.803 kb on - strand, within mutL at 561.813 kb on + strand, within mutL at 561.814 kb on - strand, within mutL at 561.814 kb on - strand, within mutL at 561.939 kb on + strand, within mutL at 561.939 kb on + strand, within mutL at 561.961 kb on + strand, within mutL at 561.962 kb on - strand, within mutL at 561.962 kb on - strand, within mutL at 561.962 kb on - strand, within mutL at 561.966 kb on - strand, within mutL at 561.966 kb on - strand, within mutL at 562.057 kb on + strand, within mutL at 562.057 kb on + strand, within mutL at 562.057 kb on + strand, within mutL at 562.057 kb on + strand, within mutL at 562.058 kb on - strand, within mutL at 562.058 kb on - strand, within mutL at 562.058 kb on - strand, within mutL
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 559,147 - hflX MCAODC_02765 0.59 -1.7 559,147 - hflX MCAODC_02765 0.59 +1.6 559,308 - hflX MCAODC_02765 0.72 +1.1 559,341 - hflX MCAODC_02765 0.74 -1.0 559,341 - hflX MCAODC_02765 0.74 +0.2 559,485 - hflX MCAODC_02765 0.86 +0.3 559,495 - hflX MCAODC_02765 0.86 -1.1 559,495 - hflX MCAODC_02765 0.86 -0.4 559,614 - +0.8 559,619 - +1.0 559,619 - +0.0 559,619 - -1.2 559,671 + -0.0 559,672 - -0.3 559,672 - -1.0 559,672 - +0.0 559,672 - +0.5 559,672 - +0.3 559,672 - -1.8 559,674 - +1.4 559,674 - -1.1 559,685 + +0.4 559,686 - -0.5 559,686 - -0.7 559,686 - -0.4 559,686 - +2.1 559,795 - hfq MCAODC_02770 0.17 -0.0 559,795 - hfq MCAODC_02770 0.17 +1.3 559,805 + hfq MCAODC_02770 0.20 +2.6 559,806 - hfq MCAODC_02770 0.20 +0.3 559,806 - hfq MCAODC_02770 0.20 +0.6 559,810 - hfq MCAODC_02770 0.21 +0.3 559,810 - hfq MCAODC_02770 0.21 +0.2 559,810 - hfq MCAODC_02770 0.21 +1.0 559,837 - hfq MCAODC_02770 0.30 -0.8 560,075 - -0.5 560,075 - +1.4 560,075 - +1.9 560,081 - +2.7 560,119 - +1.1 560,119 - +0.6 560,132 - +2.1 560,132 - -0.3 560,132 - +1.0 560,166 - -1.2 560,169 - +0.3 560,169 - +0.5 560,169 - +0.6 560,299 - miaA MCAODC_02775 0.17 +0.7 560,320 - miaA MCAODC_02775 0.19 -2.9 560,320 - miaA MCAODC_02775 0.19 +0.7 560,369 - miaA MCAODC_02775 0.24 -2.0 560,511 - miaA MCAODC_02775 0.39 -2.2 560,758 - miaA MCAODC_02775 0.65 -0.4 560,768 - miaA MCAODC_02775 0.66 -2.1 560,904 - miaA MCAODC_02775 0.81 -3.1 560,953 + miaA MCAODC_02775 0.86 +0.5 561,058 + -1.1 561,059 - +0.3 561,074 - -0.0 561,080 - -1.0 561,115 - -2.0 561,115 - -0.8 561,115 - +1.0 561,169 - -0.1 561,261 - +0.2 561,291 + mutL MCAODC_02780 0.11 -0.7 561,295 + mutL MCAODC_02780 0.12 +0.6 561,295 + mutL MCAODC_02780 0.12 +1.0 561,296 - mutL MCAODC_02780 0.12 -0.9 561,296 - mutL MCAODC_02780 0.12 -0.0 561,296 - mutL MCAODC_02780 0.12 -0.7 561,296 - mutL MCAODC_02780 0.12 -0.7 561,300 + mutL MCAODC_02780 0.12 -1.0 561,300 + mutL MCAODC_02780 0.12 +0.5 561,300 + mutL MCAODC_02780 0.12 -1.5 561,301 - mutL MCAODC_02780 0.12 -0.7 561,301 - mutL MCAODC_02780 0.12 +1.8 561,301 - mutL MCAODC_02780 0.12 +0.2 561,301 - mutL MCAODC_02780 0.12 -0.3 561,301 - mutL MCAODC_02780 0.12 -0.1 561,301 - mutL MCAODC_02780 0.12 -0.7 561,345 + mutL MCAODC_02780 0.14 +0.3 561,556 - mutL MCAODC_02780 0.26 -1.5 561,780 - mutL MCAODC_02780 0.38 +1.2 561,780 - mutL MCAODC_02780 0.38 -0.5 561,780 - mutL MCAODC_02780 0.38 -0.2 561,802 + mutL MCAODC_02780 0.39 +0.7 561,803 - mutL MCAODC_02780 0.39 -0.6 561,813 + mutL MCAODC_02780 0.40 +0.8 561,814 - mutL MCAODC_02780 0.40 +1.4 561,814 - mutL MCAODC_02780 0.40 -0.5 561,939 + mutL MCAODC_02780 0.46 -2.3 561,939 + mutL MCAODC_02780 0.46 +0.7 561,961 + mutL MCAODC_02780 0.48 -1.7 561,962 - mutL MCAODC_02780 0.48 -1.8 561,962 - mutL MCAODC_02780 0.48 +0.2 561,962 - mutL MCAODC_02780 0.48 -0.5 561,966 - mutL MCAODC_02780 0.48 -0.0 561,966 - mutL MCAODC_02780 0.48 +0.7 562,057 + mutL MCAODC_02780 0.53 +0.7 562,057 + mutL MCAODC_02780 0.53 -0.8 562,057 + mutL MCAODC_02780 0.53 -0.7 562,057 + mutL MCAODC_02780 0.53 -0.3 562,058 - mutL MCAODC_02780 0.53 +0.5 562,058 - mutL MCAODC_02780 0.53 -1.2 562,058 - mutL MCAODC_02780 0.53 -1.2
Or see this region's nucleotide sequence