Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bdcA and ridA are separated by 130 nucleotides ridA and pyrB are separated by 418 nucleotides pyrB and pyrI are separated by 12 nucleotides pyrI and ridA are separated by 72 nucleotides
MCAODC_02360: bdcA - SDR family oxidoreductase, at 484,491 to 485,204
bdcA
MCAODC_02365: ridA - Endoribonuclease L-PSP, at 485,335 to 485,730
ridA
MCAODC_02370: pyrB - aspartate carbamoyltransferase, at 486,149 to 487,084
pyrB
MCAODC_02375: pyrI - aspartate carbamoyltransferase regulatory subunit, at 487,097 to 487,558
pyrI
MCAODC_02380: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 487,631 to 488,017
ridA
Position (kb) 486
487
488 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 485.177 kb on + strand at 485.177 kb on + strand at 485.178 kb on - strand at 485.210 kb on + strand at 485.273 kb on + strand at 485.289 kb on + strand at 485.290 kb on - strand at 485.293 kb on + strand at 485.295 kb on + strand at 485.295 kb on + strand at 485.296 kb on - strand at 485.315 kb on + strand at 485.406 kb on + strand, within ridA at 485.444 kb on + strand, within ridA at 485.444 kb on + strand, within ridA at 485.444 kb on + strand, within ridA at 485.445 kb on - strand, within ridA at 485.554 kb on - strand, within ridA at 485.566 kb on - strand, within ridA at 485.577 kb on + strand, within ridA at 485.627 kb on + strand, within ridA at 485.627 kb on + strand, within ridA at 485.645 kb on - strand, within ridA at 485.670 kb on + strand, within ridA at 485.670 kb on + strand, within ridA at 485.670 kb on + strand, within ridA at 485.670 kb on + strand, within ridA at 485.671 kb on - strand, within ridA at 485.671 kb on - strand, within ridA at 485.671 kb on - strand, within ridA at 485.671 kb on - strand, within ridA at 485.728 kb on + strand at 485.729 kb on - strand at 485.735 kb on + strand at 485.767 kb on + strand at 485.767 kb on + strand at 485.768 kb on - strand at 485.768 kb on - strand at 485.769 kb on + strand at 485.770 kb on - strand at 485.982 kb on + strand at 486.010 kb on + strand at 486.010 kb on + strand at 486.011 kb on - strand at 486.011 kb on - strand at 486.011 kb on - strand at 486.011 kb on - strand at 486.011 kb on - strand at 486.011 kb on - strand at 486.176 kb on - strand at 486.176 kb on - strand at 486.386 kb on - strand, within pyrB at 486.443 kb on + strand, within pyrB at 486.443 kb on + strand, within pyrB at 486.444 kb on - strand, within pyrB at 486.444 kb on - strand, within pyrB at 486.576 kb on + strand, within pyrB at 486.576 kb on + strand, within pyrB at 486.576 kb on + strand, within pyrB at 486.576 kb on + strand, within pyrB at 486.577 kb on - strand, within pyrB at 486.577 kb on - strand, within pyrB at 486.645 kb on - strand, within pyrB at 486.705 kb on - strand, within pyrB at 486.741 kb on - strand, within pyrB at 486.793 kb on + strand, within pyrB at 486.793 kb on + strand, within pyrB at 486.827 kb on + strand, within pyrB at 486.827 kb on + strand, within pyrB at 486.827 kb on + strand, within pyrB at 486.827 kb on + strand, within pyrB at 486.828 kb on - strand, within pyrB at 486.931 kb on + strand, within pyrB at 486.939 kb on + strand, within pyrB at 486.939 kb on + strand, within pyrB at 486.940 kb on - strand, within pyrB at 486.940 kb on - strand, within pyrB at 486.985 kb on + strand, within pyrB at 487.004 kb on + strand at 487.004 kb on + strand at 487.082 kb on + strand at 487.083 kb on - strand at 487.092 kb on + strand at 487.092 kb on + strand at 487.093 kb on - strand at 487.093 kb on - strand at 487.108 kb on + strand at 487.112 kb on - strand at 487.129 kb on + strand at 487.146 kb on + strand, within pyrI at 487.177 kb on + strand, within pyrI at 487.177 kb on + strand, within pyrI at 487.178 kb on - strand, within pyrI at 487.285 kb on + strand, within pyrI at 487.308 kb on + strand, within pyrI at 487.309 kb on - strand, within pyrI at 487.325 kb on + strand, within pyrI at 487.325 kb on + strand, within pyrI at 487.326 kb on - strand, within pyrI at 487.326 kb on - strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.361 kb on + strand, within pyrI at 487.362 kb on - strand, within pyrI at 487.362 kb on - strand, within pyrI at 487.375 kb on + strand, within pyrI at 487.375 kb on + strand, within pyrI at 487.438 kb on + strand, within pyrI at 487.439 kb on - strand, within pyrI at 487.515 kb on - strand at 487.538 kb on - strand at 487.557 kb on - strand at 487.557 kb on - strand at 487.589 kb on - strand at 487.590 kb on + strand at 487.615 kb on + strand at 487.615 kb on + strand at 487.615 kb on + strand at 487.616 kb on - strand at 487.618 kb on + strand at 487.669 kb on + strand at 487.670 kb on - strand, within ridA at 487.680 kb on - strand, within ridA at 487.683 kb on + strand, within ridA at 487.683 kb on + strand, within ridA at 487.684 kb on - strand, within ridA at 487.684 kb on - strand, within ridA at 487.684 kb on - strand, within ridA at 487.705 kb on + strand, within ridA at 487.705 kb on + strand, within ridA at 487.705 kb on + strand, within ridA at 487.847 kb on - strand, within ridA at 487.853 kb on - strand, within ridA at 487.853 kb on - strand, within ridA at 487.901 kb on + strand, within ridA at 487.901 kb on + strand, within ridA at 487.902 kb on - strand, within ridA at 488.037 kb on + strand at 488.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 485,177 + -0.6 485,177 + +0.8 485,178 - -1.4 485,210 + +2.2 485,273 + -1.1 485,289 + +1.5 485,290 - -2.2 485,293 + +1.4 485,295 + -0.1 485,295 + -0.3 485,296 - -0.1 485,315 + +0.2 485,406 + ridA MCAODC_02365 0.18 -0.7 485,444 + ridA MCAODC_02365 0.28 +0.4 485,444 + ridA MCAODC_02365 0.28 -0.2 485,444 + ridA MCAODC_02365 0.28 -0.5 485,445 - ridA MCAODC_02365 0.28 +0.3 485,554 - ridA MCAODC_02365 0.55 +0.0 485,566 - ridA MCAODC_02365 0.58 -1.8 485,577 + ridA MCAODC_02365 0.61 -1.8 485,627 + ridA MCAODC_02365 0.74 -2.4 485,627 + ridA MCAODC_02365 0.74 +0.4 485,645 - ridA MCAODC_02365 0.78 -0.2 485,670 + ridA MCAODC_02365 0.85 +1.4 485,670 + ridA MCAODC_02365 0.85 -0.0 485,670 + ridA MCAODC_02365 0.85 +0.1 485,670 + ridA MCAODC_02365 0.85 -1.5 485,671 - ridA MCAODC_02365 0.85 -1.3 485,671 - ridA MCAODC_02365 0.85 +0.4 485,671 - ridA MCAODC_02365 0.85 -0.0 485,671 - ridA MCAODC_02365 0.85 +0.6 485,728 + -1.2 485,729 - -1.3 485,735 + -0.0 485,767 + -0.5 485,767 + +1.0 485,768 - -1.6 485,768 - +0.9 485,769 + +0.9 485,770 - +0.0 485,982 + -0.5 486,010 + +0.7 486,010 + +0.3 486,011 - -0.1 486,011 - -0.4 486,011 - +0.5 486,011 - -1.6 486,011 - -1.6 486,011 - -2.0 486,176 - -2.1 486,176 - -1.7 486,386 - pyrB MCAODC_02370 0.25 -2.2 486,443 + pyrB MCAODC_02370 0.31 -0.9 486,443 + pyrB MCAODC_02370 0.31 -0.1 486,444 - pyrB MCAODC_02370 0.32 -0.7 486,444 - pyrB MCAODC_02370 0.32 -0.5 486,576 + pyrB MCAODC_02370 0.46 -0.3 486,576 + pyrB MCAODC_02370 0.46 +1.1 486,576 + pyrB MCAODC_02370 0.46 -2.4 486,576 + pyrB MCAODC_02370 0.46 -0.5 486,577 - pyrB MCAODC_02370 0.46 -1.0 486,577 - pyrB MCAODC_02370 0.46 -1.9 486,645 - pyrB MCAODC_02370 0.53 -1.8 486,705 - pyrB MCAODC_02370 0.59 -0.5 486,741 - pyrB MCAODC_02370 0.63 -0.5 486,793 + pyrB MCAODC_02370 0.69 -0.1 486,793 + pyrB MCAODC_02370 0.69 -3.9 486,827 + pyrB MCAODC_02370 0.72 -0.0 486,827 + pyrB MCAODC_02370 0.72 -1.2 486,827 + pyrB MCAODC_02370 0.72 +0.2 486,827 + pyrB MCAODC_02370 0.72 -2.1 486,828 - pyrB MCAODC_02370 0.73 -1.6 486,931 + pyrB MCAODC_02370 0.84 -0.5 486,939 + pyrB MCAODC_02370 0.84 -0.7 486,939 + pyrB MCAODC_02370 0.84 +0.2 486,940 - pyrB MCAODC_02370 0.85 -0.2 486,940 - pyrB MCAODC_02370 0.85 +0.5 486,985 + pyrB MCAODC_02370 0.89 +0.1 487,004 + -2.1 487,004 + -1.1 487,082 + -1.7 487,083 - -0.5 487,092 + +1.1 487,092 + -2.0 487,093 - -0.6 487,093 - +2.1 487,108 + -1.6 487,112 - +1.5 487,129 + +0.2 487,146 + pyrI MCAODC_02375 0.11 +0.3 487,177 + pyrI MCAODC_02375 0.17 -0.1 487,177 + pyrI MCAODC_02375 0.17 -1.0 487,178 - pyrI MCAODC_02375 0.18 -1.0 487,285 + pyrI MCAODC_02375 0.41 -0.4 487,308 + pyrI MCAODC_02375 0.46 +0.0 487,309 - pyrI MCAODC_02375 0.46 -0.8 487,325 + pyrI MCAODC_02375 0.49 -0.4 487,325 + pyrI MCAODC_02375 0.49 -1.7 487,326 - pyrI MCAODC_02375 0.50 +2.0 487,326 - pyrI MCAODC_02375 0.50 +0.0 487,361 + pyrI MCAODC_02375 0.57 -1.5 487,361 + pyrI MCAODC_02375 0.57 -1.1 487,361 + pyrI MCAODC_02375 0.57 -0.3 487,361 + pyrI MCAODC_02375 0.57 +0.0 487,361 + pyrI MCAODC_02375 0.57 +0.3 487,361 + pyrI MCAODC_02375 0.57 -0.1 487,361 + pyrI MCAODC_02375 0.57 -1.9 487,362 - pyrI MCAODC_02375 0.57 -0.6 487,362 - pyrI MCAODC_02375 0.57 +0.5 487,375 + pyrI MCAODC_02375 0.60 -0.4 487,375 + pyrI MCAODC_02375 0.60 -0.1 487,438 + pyrI MCAODC_02375 0.74 -1.9 487,439 - pyrI MCAODC_02375 0.74 -2.1 487,515 - -0.3 487,538 - +1.7 487,557 - +0.2 487,557 - +3.3 487,589 - +0.9 487,590 + -2.3 487,615 + +0.1 487,615 + -0.2 487,615 + -0.8 487,616 - -0.1 487,618 + -0.7 487,669 + -0.7 487,670 - ridA MCAODC_02380 0.10 +1.3 487,680 - ridA MCAODC_02380 0.13 -0.5 487,683 + ridA MCAODC_02380 0.13 -0.8 487,683 + ridA MCAODC_02380 0.13 +0.1 487,684 - ridA MCAODC_02380 0.14 -1.8 487,684 - ridA MCAODC_02380 0.14 -0.4 487,684 - ridA MCAODC_02380 0.14 -1.0 487,705 + ridA MCAODC_02380 0.19 +2.0 487,705 + ridA MCAODC_02380 0.19 -0.2 487,705 + ridA MCAODC_02380 0.19 +0.1 487,847 - ridA MCAODC_02380 0.56 -0.2 487,853 - ridA MCAODC_02380 0.57 +0.4 487,853 - ridA MCAODC_02380 0.57 -1.6 487,901 + ridA MCAODC_02380 0.70 +1.1 487,901 + ridA MCAODC_02380 0.70 -1.9 487,902 - ridA MCAODC_02380 0.70 -0.8 488,037 + -0.7 488,052 - -1.1
Or see this region's nucleotide sequence