Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thpR and sfsA are separated by 14 nucleotides sfsA and dksA are separated by 177 nucleotides dksA and gluQRS are separated by 36 nucleotides
MCAODC_00850: thpR - RNA 2',3'-cyclic phosphodiesterase, at 150,857 to 151,387
thpR
MCAODC_00855: sfsA - DNA/RNA nuclease SfsA, at 151,402 to 152,106
sfsA
MCAODC_00860: dksA - RNA polymerase-binding protein DksA, at 152,284 to 152,739
dksA
MCAODC_00865: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 152,776 to 153,702
gluQRS
Position (kb) 152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 151.286 kb on - strand, within thpR at 151.295 kb on + strand, within thpR at 151.295 kb on + strand, within thpR at 151.296 kb on - strand, within thpR at 151.296 kb on - strand, within thpR at 151.320 kb on - strand, within thpR at 151.352 kb on + strand at 151.352 kb on + strand at 151.352 kb on + strand at 151.352 kb on + strand at 151.385 kb on + strand at 151.421 kb on + strand at 151.421 kb on + strand at 151.421 kb on + strand at 151.422 kb on - strand at 151.499 kb on + strand, within sfsA at 151.499 kb on + strand, within sfsA at 151.499 kb on + strand, within sfsA at 151.500 kb on - strand, within sfsA at 151.513 kb on - strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.589 kb on - strand, within sfsA at 151.589 kb on - strand, within sfsA at 151.608 kb on - strand, within sfsA at 151.677 kb on + strand, within sfsA at 151.683 kb on + strand, within sfsA at 151.712 kb on - strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.759 kb on - strand, within sfsA at 151.767 kb on + strand, within sfsA at 151.767 kb on + strand, within sfsA at 151.768 kb on - strand, within sfsA at 151.768 kb on - strand, within sfsA at 151.798 kb on + strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.801 kb on - strand, within sfsA at 151.801 kb on - strand, within sfsA at 151.818 kb on + strand, within sfsA at 151.819 kb on - strand, within sfsA at 151.819 kb on - strand, within sfsA at 151.843 kb on + strand, within sfsA at 151.957 kb on - strand, within sfsA at 151.993 kb on + strand, within sfsA at 151.994 kb on - strand, within sfsA at 151.994 kb on - strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.044 kb on + strand at 152.045 kb on - strand at 152.080 kb on - strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.108 kb on - strand at 152.112 kb on + strand at 152.112 kb on + strand at 152.113 kb on - strand at 152.116 kb on + strand at 152.117 kb on - strand at 152.153 kb on - strand at 152.182 kb on + strand at 152.183 kb on - strand at 152.188 kb on + strand at 152.219 kb on - strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.223 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.374 kb on + strand, within dksA at 152.374 kb on + strand, within dksA at 152.375 kb on - strand, within dksA at 152.376 kb on + strand, within dksA at 152.460 kb on + strand, within dksA at 152.460 kb on + strand, within dksA at 152.460 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.466 kb on + strand, within dksA at 152.467 kb on - strand, within dksA at 152.467 kb on - strand, within dksA at 152.467 kb on - strand, within dksA at 152.562 kb on + strand, within dksA at 152.649 kb on + strand, within dksA at 152.741 kb on + strand at 152.741 kb on + strand at 152.741 kb on + strand at 152.762 kb on - strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.821 kb on + strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.822 kb on - strand at 152.823 kb on + strand at 152.823 kb on + strand at 152.893 kb on + strand, within gluQRS at 152.894 kb on - strand, within gluQRS at 152.894 kb on - strand, within gluQRS at 152.944 kb on - strand, within gluQRS at 152.982 kb on + strand, within gluQRS at 152.983 kb on - strand, within gluQRS at 152.983 kb on - strand, within gluQRS at 153.004 kb on + strand, within gluQRS at 153.080 kb on + strand, within gluQRS at 153.080 kb on + strand, within gluQRS at 153.095 kb on + strand, within gluQRS at 153.095 kb on + strand, within gluQRS at 153.095 kb on + strand, within gluQRS at 153.101 kb on - strand, within gluQRS at 153.101 kb on - strand, within gluQRS at 153.101 kb on - strand, within gluQRS at 153.103 kb on + strand, within gluQRS at 153.126 kb on + strand, within gluQRS at 153.148 kb on + strand, within gluQRS at 153.149 kb on - strand, within gluQRS at 153.231 kb on + strand, within gluQRS at 153.252 kb on + strand, within gluQRS at 153.288 kb on + strand, within gluQRS at 153.289 kb on - strand, within gluQRS at 153.319 kb on + strand, within gluQRS at 153.319 kb on + strand, within gluQRS at 153.320 kb on - strand, within gluQRS at 153.419 kb on - strand, within gluQRS at 153.448 kb on + strand, within gluQRS at 153.449 kb on - strand, within gluQRS at 153.449 kb on - strand, within gluQRS at 153.545 kb on + strand, within gluQRS at 153.546 kb on - strand, within gluQRS at 153.546 kb on - strand, within gluQRS at 153.706 kb on - strand at 153.706 kb on - strand at 153.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 151,286 - thpR MCAODC_00850 0.81 -0.0 151,295 + thpR MCAODC_00850 0.82 +0.8 151,295 + thpR MCAODC_00850 0.82 -1.1 151,296 - thpR MCAODC_00850 0.83 -1.8 151,296 - thpR MCAODC_00850 0.83 -0.9 151,320 - thpR MCAODC_00850 0.87 -1.0 151,352 + +0.7 151,352 + -0.3 151,352 + +0.5 151,352 + -1.9 151,385 + +0.4 151,421 + -1.7 151,421 + +1.4 151,421 + +0.2 151,422 - -0.5 151,499 + sfsA MCAODC_00855 0.14 +0.4 151,499 + sfsA MCAODC_00855 0.14 -1.2 151,499 + sfsA MCAODC_00855 0.14 +0.1 151,500 - sfsA MCAODC_00855 0.14 +0.7 151,513 - sfsA MCAODC_00855 0.16 +0.0 151,588 + sfsA MCAODC_00855 0.26 -0.9 151,588 + sfsA MCAODC_00855 0.26 -0.7 151,588 + sfsA MCAODC_00855 0.26 -1.1 151,589 - sfsA MCAODC_00855 0.27 +0.4 151,589 - sfsA MCAODC_00855 0.27 +0.3 151,608 - sfsA MCAODC_00855 0.29 -0.5 151,677 + sfsA MCAODC_00855 0.39 -1.0 151,683 + sfsA MCAODC_00855 0.40 -0.6 151,712 - sfsA MCAODC_00855 0.44 +1.6 151,713 + sfsA MCAODC_00855 0.44 -1.8 151,713 + sfsA MCAODC_00855 0.44 +1.1 151,713 + sfsA MCAODC_00855 0.44 +0.4 151,714 - sfsA MCAODC_00855 0.44 +0.5 151,714 - sfsA MCAODC_00855 0.44 -0.5 151,714 - sfsA MCAODC_00855 0.44 -0.1 151,759 - sfsA MCAODC_00855 0.51 +0.9 151,767 + sfsA MCAODC_00855 0.52 -0.1 151,767 + sfsA MCAODC_00855 0.52 -1.5 151,768 - sfsA MCAODC_00855 0.52 -1.1 151,768 - sfsA MCAODC_00855 0.52 +0.4 151,798 + sfsA MCAODC_00855 0.56 +0.1 151,799 - sfsA MCAODC_00855 0.56 -1.2 151,799 - sfsA MCAODC_00855 0.56 +0.6 151,799 - sfsA MCAODC_00855 0.56 +0.7 151,799 - sfsA MCAODC_00855 0.56 -0.6 151,799 - sfsA MCAODC_00855 0.56 +0.5 151,799 - sfsA MCAODC_00855 0.56 -0.1 151,801 - sfsA MCAODC_00855 0.57 -0.9 151,801 - sfsA MCAODC_00855 0.57 +0.1 151,818 + sfsA MCAODC_00855 0.59 +1.1 151,819 - sfsA MCAODC_00855 0.59 +0.5 151,819 - sfsA MCAODC_00855 0.59 -0.4 151,843 + sfsA MCAODC_00855 0.63 -1.9 151,957 - sfsA MCAODC_00855 0.79 -1.8 151,993 + sfsA MCAODC_00855 0.84 +0.6 151,994 - sfsA MCAODC_00855 0.84 -0.3 151,994 - sfsA MCAODC_00855 0.84 +1.1 152,003 + sfsA MCAODC_00855 0.85 +0.8 152,003 + sfsA MCAODC_00855 0.85 +0.7 152,003 + sfsA MCAODC_00855 0.85 +1.0 152,004 - sfsA MCAODC_00855 0.85 +0.4 152,004 - sfsA MCAODC_00855 0.85 +0.1 152,004 - sfsA MCAODC_00855 0.85 -0.1 152,004 - sfsA MCAODC_00855 0.85 -0.7 152,004 - sfsA MCAODC_00855 0.85 -0.7 152,044 + +0.3 152,045 - +0.9 152,080 - -1.6 152,104 + +0.8 152,104 + -1.7 152,104 + -0.3 152,104 + +0.9 152,104 + +0.7 152,105 - +0.2 152,105 - +0.1 152,105 - -0.2 152,105 - -0.2 152,108 - -0.3 152,112 + +0.8 152,112 + -0.8 152,113 - +1.1 152,116 + +0.6 152,117 - -0.1 152,153 - -0.1 152,182 + -1.5 152,183 - -0.4 152,188 + +0.5 152,219 - -0.1 152,222 + -0.3 152,222 + +2.0 152,222 + -1.7 152,222 + -1.5 152,223 - -2.1 152,224 + +0.5 152,224 + -3.0 152,374 + dksA MCAODC_00860 0.20 -1.8 152,374 + dksA MCAODC_00860 0.20 -1.6 152,375 - dksA MCAODC_00860 0.20 +1.5 152,376 + dksA MCAODC_00860 0.20 -2.0 152,460 + dksA MCAODC_00860 0.39 -3.0 152,460 + dksA MCAODC_00860 0.39 -3.1 152,460 + dksA MCAODC_00860 0.39 -1.7 152,466 + dksA MCAODC_00860 0.40 -1.9 152,466 + dksA MCAODC_00860 0.40 -2.3 152,466 + dksA MCAODC_00860 0.40 -2.8 152,466 + dksA MCAODC_00860 0.40 -2.6 152,466 + dksA MCAODC_00860 0.40 +0.6 152,466 + dksA MCAODC_00860 0.40 -3.7 152,466 + dksA MCAODC_00860 0.40 -1.3 152,466 + dksA MCAODC_00860 0.40 -1.4 152,467 - dksA MCAODC_00860 0.40 +0.2 152,467 - dksA MCAODC_00860 0.40 -0.1 152,467 - dksA MCAODC_00860 0.40 -0.3 152,562 + dksA MCAODC_00860 0.61 -2.8 152,649 + dksA MCAODC_00860 0.80 -0.8 152,741 + -0.3 152,741 + -1.7 152,741 + -1.0 152,762 - -1.1 152,821 + +0.6 152,821 + -1.6 152,821 + +0.1 152,821 + -0.1 152,821 + +0.3 152,821 + -0.2 152,821 + +0.0 152,821 + +2.0 152,821 + +0.1 152,822 - -0.1 152,822 - -1.6 152,822 - -2.7 152,822 - -1.7 152,822 - -0.8 152,822 - +0.0 152,822 - -0.1 152,822 - -0.7 152,822 - +2.2 152,823 + +0.3 152,823 + +0.3 152,893 + gluQRS MCAODC_00865 0.13 +0.1 152,894 - gluQRS MCAODC_00865 0.13 -0.6 152,894 - gluQRS MCAODC_00865 0.13 -0.4 152,944 - gluQRS MCAODC_00865 0.18 -0.9 152,982 + gluQRS MCAODC_00865 0.22 -0.0 152,983 - gluQRS MCAODC_00865 0.22 -1.1 152,983 - gluQRS MCAODC_00865 0.22 +0.2 153,004 + gluQRS MCAODC_00865 0.25 -0.4 153,080 + gluQRS MCAODC_00865 0.33 +0.5 153,080 + gluQRS MCAODC_00865 0.33 -1.8 153,095 + gluQRS MCAODC_00865 0.34 -1.5 153,095 + gluQRS MCAODC_00865 0.34 -0.2 153,095 + gluQRS MCAODC_00865 0.34 +1.2 153,101 - gluQRS MCAODC_00865 0.35 -2.1 153,101 - gluQRS MCAODC_00865 0.35 +0.1 153,101 - gluQRS MCAODC_00865 0.35 -0.3 153,103 + gluQRS MCAODC_00865 0.35 -0.4 153,126 + gluQRS MCAODC_00865 0.38 +1.1 153,148 + gluQRS MCAODC_00865 0.40 +0.5 153,149 - gluQRS MCAODC_00865 0.40 -0.4 153,231 + gluQRS MCAODC_00865 0.49 -0.5 153,252 + gluQRS MCAODC_00865 0.51 +0.0 153,288 + gluQRS MCAODC_00865 0.55 +0.8 153,289 - gluQRS MCAODC_00865 0.55 +0.3 153,319 + gluQRS MCAODC_00865 0.59 -0.6 153,319 + gluQRS MCAODC_00865 0.59 -0.1 153,320 - gluQRS MCAODC_00865 0.59 +0.3 153,419 - gluQRS MCAODC_00865 0.69 -0.4 153,448 + gluQRS MCAODC_00865 0.72 -0.2 153,449 - gluQRS MCAODC_00865 0.73 -0.0 153,449 - gluQRS MCAODC_00865 0.73 +0.2 153,545 + gluQRS MCAODC_00865 0.83 -0.7 153,546 - gluQRS MCAODC_00865 0.83 -0.6 153,546 - gluQRS MCAODC_00865 0.83 +0.1 153,706 - -1.0 153,706 - +0.0 153,712 - -1.3
Or see this region's nucleotide sequence