Experiment: WM_EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeF and nlpE are separated by 199 nucleotides nlpE and arfB are separated by 13 nucleotides arfB and yaeQ overlap by 4 nucleotides yaeQ and yaeP are separated by 165 nucleotides yaeP and rof overlap by 14 nucleotides
MCAODC_00595: yaeF - YaeF family permuted papain-like enzyme, at 96,875 to 97,699
yaeF
MCAODC_00605: nlpE - envelope stress response activation lipoprotein NlpE, at 97,899 to 98,609
nlpE
MCAODC_00610: arfB - aminoacyl-tRNA hydrolase, at 98,623 to 99,033
arfB
MCAODC_00615: yaeQ - Uncharacterized protein YaeQ, at 99,030 to 99,575
yaeQ
MCAODC_00620: yaeP - YaeP family protein, at 99,741 to 99,941
yaeP
MCAODC_00625: rof - Rho-binding antiterminator, at 99,928 to 100,188
rof
Position (kb) 98
99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 97.629 kb on + strand at 97.630 kb on - strand at 97.630 kb on - strand at 97.634 kb on + strand at 97.634 kb on + strand at 97.635 kb on - strand at 97.661 kb on + strand at 97.661 kb on + strand at 97.661 kb on + strand at 97.662 kb on - strand at 97.662 kb on - strand at 97.701 kb on - strand at 97.705 kb on + strand at 97.706 kb on - strand at 97.706 kb on - strand at 97.706 kb on - strand at 97.723 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.743 kb on - strand at 97.752 kb on - strand at 97.752 kb on - strand at 97.752 kb on - strand at 97.913 kb on + strand at 97.959 kb on + strand at 97.960 kb on - strand at 97.962 kb on + strand at 97.962 kb on + strand at 97.963 kb on - strand at 97.992 kb on - strand, within nlpE at 98.016 kb on + strand, within nlpE at 98.016 kb on + strand, within nlpE at 98.016 kb on + strand, within nlpE at 98.016 kb on + strand, within nlpE at 98.017 kb on - strand, within nlpE at 98.017 kb on - strand, within nlpE at 98.017 kb on - strand, within nlpE at 98.017 kb on - strand, within nlpE at 98.025 kb on + strand, within nlpE at 98.026 kb on - strand, within nlpE at 98.146 kb on + strand, within nlpE at 98.152 kb on + strand, within nlpE at 98.152 kb on + strand, within nlpE at 98.185 kb on - strand, within nlpE at 98.211 kb on - strand, within nlpE at 98.212 kb on + strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.213 kb on - strand, within nlpE at 98.281 kb on + strand, within nlpE at 98.282 kb on - strand, within nlpE at 98.284 kb on + strand, within nlpE at 98.284 kb on + strand, within nlpE at 98.313 kb on + strand, within nlpE at 98.342 kb on + strand, within nlpE at 98.342 kb on + strand, within nlpE at 98.343 kb on - strand, within nlpE at 98.343 kb on - strand, within nlpE at 98.347 kb on + strand, within nlpE at 98.526 kb on - strand, within nlpE at 98.526 kb on - strand, within nlpE at 98.544 kb on - strand at 98.547 kb on - strand at 98.574 kb on + strand at 98.575 kb on - strand at 98.740 kb on + strand, within arfB at 98.741 kb on - strand, within arfB at 98.741 kb on - strand, within arfB at 98.749 kb on - strand, within arfB at 98.818 kb on - strand, within arfB at 98.827 kb on + strand, within arfB at 98.827 kb on + strand, within arfB at 98.879 kb on + strand, within arfB at 98.882 kb on + strand, within arfB at 98.940 kb on + strand, within arfB at 98.943 kb on + strand, within arfB at 98.943 kb on + strand, within arfB at 98.944 kb on - strand, within arfB at 98.944 kb on - strand, within arfB at 98.944 kb on - strand, within arfB at 99.006 kb on + strand at 99.072 kb on - strand at 99.077 kb on + strand at 99.078 kb on - strand at 99.132 kb on - strand, within yaeQ at 99.132 kb on - strand, within yaeQ at 99.178 kb on + strand, within yaeQ at 99.179 kb on - strand, within yaeQ at 99.251 kb on + strand, within yaeQ at 99.251 kb on + strand, within yaeQ at 99.252 kb on - strand, within yaeQ at 99.254 kb on + strand, within yaeQ at 99.254 kb on + strand, within yaeQ at 99.255 kb on - strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.256 kb on + strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.257 kb on - strand, within yaeQ at 99.260 kb on + strand, within yaeQ at 99.260 kb on + strand, within yaeQ at 99.261 kb on - strand, within yaeQ at 99.261 kb on - strand, within yaeQ at 99.427 kb on + strand, within yaeQ at 99.427 kb on + strand, within yaeQ at 99.428 kb on - strand, within yaeQ at 99.428 kb on - strand, within yaeQ at 99.458 kb on + strand, within yaeQ at 99.458 kb on + strand, within yaeQ at 99.459 kb on - strand, within yaeQ at 99.540 kb on - strand at 99.551 kb on + strand at 99.554 kb on - strand at 99.554 kb on - strand at 99.575 kb on + strand at 99.616 kb on + strand at 99.616 kb on + strand at 99.617 kb on - strand at 99.617 kb on - strand at 99.637 kb on + strand at 99.638 kb on - strand at 99.638 kb on - strand at 99.657 kb on + strand at 99.657 kb on + strand at 99.658 kb on - strand at 99.658 kb on - strand at 99.658 kb on - strand at 99.659 kb on + strand at 99.659 kb on + strand at 99.683 kb on - strand at 99.691 kb on - strand at 99.696 kb on + strand at 99.696 kb on + strand at 99.697 kb on - strand at 99.763 kb on + strand, within yaeP at 99.799 kb on + strand, within yaeP at 99.799 kb on + strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.800 kb on - strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.804 kb on + strand, within yaeP at 99.805 kb on - strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.897 kb on + strand, within yaeP at 99.928 kb on - strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.946 kb on + strand at 99.947 kb on - strand at 99.947 kb on - strand at 99.970 kb on + strand, within rof at 99.970 kb on + strand, within rof at 99.970 kb on + strand, within rof at 99.971 kb on - strand, within rof at 100.004 kb on + strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof at 100.005 kb on - strand, within rof
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV240 remove 97,629 + -2.4 97,630 - +0.0 97,630 - +0.9 97,634 + -1.3 97,634 + -0.9 97,635 - +0.3 97,661 + +0.3 97,661 + -1.7 97,661 + +0.0 97,662 - +2.0 97,662 - +1.4 97,701 - -1.3 97,705 + -1.5 97,706 - -0.6 97,706 - +0.3 97,706 - +0.4 97,723 + -2.1 97,728 + +0.5 97,728 + -1.2 97,728 + -0.6 97,728 + -0.5 97,743 - +0.1 97,752 - -0.2 97,752 - -0.0 97,752 - +1.2 97,913 + -0.8 97,959 + +0.1 97,960 - +1.0 97,962 + +0.7 97,962 + +0.1 97,963 - -0.4 97,992 - nlpE MCAODC_00605 0.13 -1.8 98,016 + nlpE MCAODC_00605 0.16 +0.7 98,016 + nlpE MCAODC_00605 0.16 +0.9 98,016 + nlpE MCAODC_00605 0.16 -0.6 98,016 + nlpE MCAODC_00605 0.16 +0.7 98,017 - nlpE MCAODC_00605 0.17 -0.5 98,017 - nlpE MCAODC_00605 0.17 +0.2 98,017 - nlpE MCAODC_00605 0.17 -0.7 98,017 - nlpE MCAODC_00605 0.17 -0.2 98,025 + nlpE MCAODC_00605 0.18 +0.6 98,026 - nlpE MCAODC_00605 0.18 -0.4 98,146 + nlpE MCAODC_00605 0.35 +0.9 98,152 + nlpE MCAODC_00605 0.36 +1.4 98,152 + nlpE MCAODC_00605 0.36 +1.0 98,185 - nlpE MCAODC_00605 0.40 -0.1 98,211 - nlpE MCAODC_00605 0.44 -0.0 98,212 + nlpE MCAODC_00605 0.44 +1.5 98,213 - nlpE MCAODC_00605 0.44 +0.9 98,213 - nlpE MCAODC_00605 0.44 -0.0 98,213 - nlpE MCAODC_00605 0.44 -1.5 98,213 - nlpE MCAODC_00605 0.44 -0.5 98,281 + nlpE MCAODC_00605 0.54 -0.4 98,282 - nlpE MCAODC_00605 0.54 -0.2 98,284 + nlpE MCAODC_00605 0.54 -0.5 98,284 + nlpE MCAODC_00605 0.54 +1.2 98,313 + nlpE MCAODC_00605 0.58 -1.4 98,342 + nlpE MCAODC_00605 0.62 -0.7 98,342 + nlpE MCAODC_00605 0.62 -1.7 98,343 - nlpE MCAODC_00605 0.62 -0.2 98,343 - nlpE MCAODC_00605 0.62 -0.1 98,347 + nlpE MCAODC_00605 0.63 +1.0 98,526 - nlpE MCAODC_00605 0.88 +0.0 98,526 - nlpE MCAODC_00605 0.88 -0.4 98,544 - +1.0 98,547 - +0.4 98,574 + +2.2 98,575 - -0.2 98,740 + arfB MCAODC_00610 0.28 -1.3 98,741 - arfB MCAODC_00610 0.29 +0.2 98,741 - arfB MCAODC_00610 0.29 -0.9 98,749 - arfB MCAODC_00610 0.31 -0.9 98,818 - arfB MCAODC_00610 0.47 -0.0 98,827 + arfB MCAODC_00610 0.50 +1.8 98,827 + arfB MCAODC_00610 0.50 +0.6 98,879 + arfB MCAODC_00610 0.62 +0.2 98,882 + arfB MCAODC_00610 0.63 -0.2 98,940 + arfB MCAODC_00610 0.77 -0.1 98,943 + arfB MCAODC_00610 0.78 -1.2 98,943 + arfB MCAODC_00610 0.78 +0.8 98,944 - arfB MCAODC_00610 0.78 -0.0 98,944 - arfB MCAODC_00610 0.78 +0.3 98,944 - arfB MCAODC_00610 0.78 -1.1 99,006 + +1.6 99,072 - +1.0 99,077 + -1.1 99,078 - +0.8 99,132 - yaeQ MCAODC_00615 0.19 +0.1 99,132 - yaeQ MCAODC_00615 0.19 +0.9 99,178 + yaeQ MCAODC_00615 0.27 -0.4 99,179 - yaeQ MCAODC_00615 0.27 -0.5 99,251 + yaeQ MCAODC_00615 0.40 -1.4 99,251 + yaeQ MCAODC_00615 0.40 +0.4 99,252 - yaeQ MCAODC_00615 0.41 +0.6 99,254 + yaeQ MCAODC_00615 0.41 -1.9 99,254 + yaeQ MCAODC_00615 0.41 -0.8 99,255 - yaeQ MCAODC_00615 0.41 +1.6 99,256 + yaeQ MCAODC_00615 0.41 -0.3 99,256 + yaeQ MCAODC_00615 0.41 -1.3 99,256 + yaeQ MCAODC_00615 0.41 +1.2 99,257 - yaeQ MCAODC_00615 0.42 +0.3 99,257 - yaeQ MCAODC_00615 0.42 +0.2 99,257 - yaeQ MCAODC_00615 0.42 +1.9 99,257 - yaeQ MCAODC_00615 0.42 +0.7 99,260 + yaeQ MCAODC_00615 0.42 +0.3 99,260 + yaeQ MCAODC_00615 0.42 +0.4 99,261 - yaeQ MCAODC_00615 0.42 +0.1 99,261 - yaeQ MCAODC_00615 0.42 -1.9 99,427 + yaeQ MCAODC_00615 0.73 +0.2 99,427 + yaeQ MCAODC_00615 0.73 +0.3 99,428 - yaeQ MCAODC_00615 0.73 +0.4 99,428 - yaeQ MCAODC_00615 0.73 -0.0 99,458 + yaeQ MCAODC_00615 0.78 +0.2 99,458 + yaeQ MCAODC_00615 0.78 +0.4 99,459 - yaeQ MCAODC_00615 0.79 +0.5 99,540 - +1.2 99,551 + -0.2 99,554 - +0.1 99,554 - -0.3 99,575 + +0.6 99,616 + -1.4 99,616 + +0.5 99,617 - -0.3 99,617 - +2.1 99,637 + -0.0 99,638 - +1.0 99,638 - +0.7 99,657 + +0.6 99,657 + +0.6 99,658 - -1.6 99,658 - +0.4 99,658 - +0.9 99,659 + +1.0 99,659 + -0.4 99,683 - -0.7 99,691 - +0.6 99,696 + +0.4 99,696 + -0.2 99,697 - -0.3 99,763 + yaeP MCAODC_00620 0.11 +1.0 99,799 + yaeP MCAODC_00620 0.29 -1.5 99,799 + yaeP MCAODC_00620 0.29 -1.1 99,800 - yaeP MCAODC_00620 0.29 +0.5 99,800 - yaeP MCAODC_00620 0.29 +1.2 99,800 - yaeP MCAODC_00620 0.29 +0.6 99,800 - yaeP MCAODC_00620 0.29 +0.5 99,800 - yaeP MCAODC_00620 0.29 +0.5 99,804 + yaeP MCAODC_00620 0.31 -0.0 99,804 + yaeP MCAODC_00620 0.31 +0.5 99,804 + yaeP MCAODC_00620 0.31 -0.1 99,805 - yaeP MCAODC_00620 0.32 -1.3 99,897 + yaeP MCAODC_00620 0.78 -0.1 99,897 + yaeP MCAODC_00620 0.78 -0.5 99,897 + yaeP MCAODC_00620 0.78 -1.5 99,928 - -0.3 99,946 + -0.2 99,946 + +0.2 99,946 + +1.1 99,946 + -1.5 99,946 + +0.0 99,947 - +0.0 99,947 - +0.5 99,970 + rof MCAODC_00625 0.16 -1.3 99,970 + rof MCAODC_00625 0.16 +0.1 99,970 + rof MCAODC_00625 0.16 -1.8 99,971 - rof MCAODC_00625 0.16 +0.8 100,004 + rof MCAODC_00625 0.29 -0.7 100,005 - rof MCAODC_00625 0.30 +0.4 100,005 - rof MCAODC_00625 0.30 -3.0 100,005 - rof MCAODC_00625 0.30 -2.7 100,005 - rof MCAODC_00625 0.30 -0.7
Or see this region's nucleotide sequence