Strain Fitness in Escherichia coli ECRC101 around MCAODC_20215

Experiment: WM_Bas41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntamn and ycdB are separated by 342 nucleotidesycdB and Asn_trna are separated by 449 nucleotidesAsn_trna and yeeO are separated by 100 nucleotides MCAODC_20210: amn - AMP nucleosidase, at 853,739 to 855,193 amn MCAODC_20215: ycdB - putative transcriptional regulatory protein YcdB, at 855,536 to 856,252 ycdB MCAODC_20220: Asn_trna - tRNA-Asn(gtt), at 856,702 to 856,777 _trna MCAODC_20225: yeeO - toxic metabolite efflux MATE transporter YeeO, at 856,878 to 858,332 yeeO Position (kb) 855 856 857Strain fitness (log2 ratio) -1 0 1 2 3at 854.554 kb on + strand, within amnat 854.555 kb on - strand, within amnat 854.557 kb on + strand, within amnat 854.557 kb on + strand, within amnat 854.557 kb on + strand, within amnat 854.570 kb on - strand, within amnat 854.593 kb on + strand, within amnat 854.622 kb on + strand, within amnat 854.752 kb on + strand, within amnat 854.852 kb on - strand, within amnat 854.858 kb on - strand, within amnat 854.892 kb on + strand, within amnat 855.023 kb on - strand, within amnat 855.172 kb on + strandat 855.191 kb on + strandat 855.194 kb on + strandat 855.254 kb on + strandat 856.042 kb on + strand, within ycdBat 856.046 kb on + strand, within ycdBat 856.345 kb on - strandat 856.679 kb on - strandat 856.769 kb on + strand, within Asn_trnaat 857.102 kb on + strand, within yeeOat 857.130 kb on - strand, within yeeOat 857.130 kb on - strand, within yeeOat 857.143 kb on - strand, within yeeOat 857.172 kb on - strand, within yeeOat 857.213 kb on + strand, within yeeO

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas41
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854,554 + amn MCAODC_20210 0.56 +1.2
854,555 - amn MCAODC_20210 0.56 -1.4
854,557 + amn MCAODC_20210 0.56 -1.0
854,557 + amn MCAODC_20210 0.56 +0.6
854,557 + amn MCAODC_20210 0.56 -0.7
854,570 - amn MCAODC_20210 0.57 -0.1
854,593 + amn MCAODC_20210 0.59 +0.2
854,622 + amn MCAODC_20210 0.61 +1.0
854,752 + amn MCAODC_20210 0.70 +3.3
854,852 - amn MCAODC_20210 0.76 -0.9
854,858 - amn MCAODC_20210 0.77 +1.3
854,892 + amn MCAODC_20210 0.79 +1.5
855,023 - amn MCAODC_20210 0.88 +0.8
855,172 + -1.2
855,191 + -0.5
855,194 + +1.2
855,254 + +0.1
856,042 + ycdB MCAODC_20215 0.71 +0.3
856,046 + ycdB MCAODC_20215 0.71 -0.0
856,345 - +1.8
856,679 - -0.2
856,769 + Asn_trna MCAODC_20220 0.88 +0.6
857,102 + yeeO MCAODC_20225 0.15 +0.2
857,130 - yeeO MCAODC_20225 0.17 -1.2
857,130 - yeeO MCAODC_20225 0.17 -0.1
857,143 - yeeO MCAODC_20225 0.18 -0.7
857,172 - yeeO MCAODC_20225 0.20 -0.2
857,213 + yeeO MCAODC_20225 0.23 +1.5

Or see this region's nucleotide sequence