Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ttcA and uspF are separated by 175 nucleotides uspF and MCAODC_16110 are separated by 140 nucleotides MCAODC_16110 and MCAODC_16115 overlap by 26 nucleotides MCAODC_16115 and nifJ are separated by 365 nucleotides
MCAODC_16100: ttcA - tRNA 2-thiocytidine(32) synthetase TtcA, at 109,160 to 110,095
ttcA
MCAODC_16105: uspF - universal stress protein UspF, at 110,271 to 110,705
uspF
MCAODC_16110: MCAODC_16110 - hypothetical protein, at 110,846 to 111,613
_16110
MCAODC_16115: MCAODC_16115 - Putative outer membrane protein, at 111,588 to 111,959
_16115
MCAODC_16125: nifJ - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 112,325 to 115,849
nifJ
Position (kb)
110
111
112 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 109.938 kb on + strand, within ttcA at 109.938 kb on + strand, within ttcA at 109.939 kb on - strand, within ttcA at 109.939 kb on - strand, within ttcA at 109.939 kb on - strand, within ttcA at 109.939 kb on - strand, within ttcA at 110.058 kb on + strand at 110.098 kb on - strand at 110.160 kb on + strand at 110.190 kb on + strand at 110.191 kb on - strand at 110.191 kb on - strand at 110.191 kb on - strand at 110.310 kb on + strand at 110.379 kb on - strand, within uspF at 110.379 kb on - strand, within uspF at 110.566 kb on + strand, within uspF at 110.566 kb on + strand, within uspF at 110.567 kb on - strand, within uspF at 110.567 kb on - strand, within uspF at 110.705 kb on + strand at 110.788 kb on + strand at 110.788 kb on + strand at 110.867 kb on + strand at 110.878 kb on + strand at 110.878 kb on + strand at 110.907 kb on + strand at 110.952 kb on + strand, within MCAODC_16110 at 110.979 kb on + strand, within MCAODC_16110 at 111.190 kb on + strand, within MCAODC_16110 at 111.191 kb on - strand, within MCAODC_16110 at 111.223 kb on - strand, within MCAODC_16110 at 111.227 kb on - strand, within MCAODC_16110 at 111.289 kb on + strand, within MCAODC_16110 at 111.363 kb on + strand, within MCAODC_16110 at 111.440 kb on - strand, within MCAODC_16110 at 111.458 kb on - strand, within MCAODC_16110 at 111.458 kb on - strand, within MCAODC_16110 at 111.569 kb on - strand at 111.614 kb on - strand at 111.618 kb on - strand at 112.068 kb on - strand at 112.199 kb on + strand at 112.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 109,938 + ttcA MCAODC_16100 0.83 -0.7 109,938 + ttcA MCAODC_16100 0.83 +1.6 109,939 - ttcA MCAODC_16100 0.83 -0.4 109,939 - ttcA MCAODC_16100 0.83 +0.3 109,939 - ttcA MCAODC_16100 0.83 -0.2 109,939 - ttcA MCAODC_16100 0.83 +0.6 110,058 + -0.1 110,098 - +0.3 110,160 + +1.6 110,190 + +1.0 110,191 - -0.1 110,191 - -0.2 110,191 - +0.1 110,310 + -0.8 110,379 - uspF MCAODC_16105 0.25 +0.2 110,379 - uspF MCAODC_16105 0.25 -1.5 110,566 + uspF MCAODC_16105 0.68 +0.2 110,566 + uspF MCAODC_16105 0.68 +0.9 110,567 - uspF MCAODC_16105 0.68 -1.1 110,567 - uspF MCAODC_16105 0.68 -0.4 110,705 + +0.9 110,788 + -1.1 110,788 + +0.3 110,867 + -0.5 110,878 + +1.6 110,878 + -2.0 110,907 + -0.2 110,952 + MCAODC_16110 0.14 -0.3 110,979 + MCAODC_16110 0.17 +0.6 111,190 + MCAODC_16110 0.45 +0.2 111,191 - MCAODC_16110 0.45 -2.0 111,223 - MCAODC_16110 0.49 -2.7 111,227 - MCAODC_16110 0.50 +0.6 111,289 + MCAODC_16110 0.58 +0.6 111,363 + MCAODC_16110 0.67 -1.5 111,440 - MCAODC_16110 0.77 +2.0 111,458 - MCAODC_16110 0.80 +0.2 111,458 - MCAODC_16110 0.80 +0.9 111,569 - -1.0 111,614 - +0.5 111,618 - -0.7 112,068 - -0.9 112,199 + -0.3 112,563 - +1.2
Or see this region's nucleotide sequence