Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapA and MCAODC_15815 are separated by 312 nucleotides MCAODC_15815 and puuP are separated by 133 nucleotides puuP and puuA are separated by 302 nucleotides
MCAODC_15810: sapA - peptide ABC transporter substrate-binding protein SapA, at 53,388 to 55,031
sapA
MCAODC_15815: MCAODC_15815 - DUF2543 domain-containing protein, at 55,344 to 55,589
_15815
MCAODC_15820: puuP - putrescine/proton symporter PuuP, at 55,723 to 57,108
puuP
MCAODC_15825: puuA - glutamate-putrescine ligase, at 57,411 to 58,829
puuA
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 55.188 kb on - strand at 55.194 kb on - strand at 55.194 kb on - strand at 55.194 kb on - strand at 55.194 kb on - strand at 55.194 kb on - strand at 55.194 kb on - strand at 55.196 kb on - strand at 55.196 kb on - strand at 55.196 kb on - strand at 55.196 kb on - strand at 55.401 kb on - strand, within MCAODC_15815 at 55.486 kb on + strand, within MCAODC_15815 at 55.654 kb on + strand at 55.654 kb on + strand at 55.664 kb on + strand at 55.664 kb on + strand at 55.666 kb on + strand at 55.725 kb on - strand at 55.725 kb on - strand at 55.725 kb on - strand at 55.725 kb on - strand at 55.725 kb on - strand at 55.725 kb on - strand at 55.758 kb on - strand at 55.800 kb on - strand at 55.933 kb on - strand, within puuP at 56.011 kb on - strand, within puuP at 56.032 kb on + strand, within puuP at 56.159 kb on + strand, within puuP at 56.159 kb on + strand, within puuP at 56.160 kb on - strand, within puuP at 56.261 kb on + strand, within puuP at 56.331 kb on + strand, within puuP at 56.366 kb on + strand, within puuP at 56.366 kb on + strand, within puuP at 56.367 kb on - strand, within puuP at 56.517 kb on + strand, within puuP at 56.560 kb on - strand, within puuP at 56.586 kb on + strand, within puuP at 56.617 kb on + strand, within puuP at 56.628 kb on + strand, within puuP at 56.808 kb on + strand, within puuP at 56.844 kb on - strand, within puuP at 56.847 kb on + strand, within puuP at 56.848 kb on - strand, within puuP at 56.969 kb on - strand, within puuP at 57.009 kb on - strand at 57.108 kb on + strand at 57.129 kb on - strand at 57.132 kb on - strand at 57.251 kb on - strand at 57.520 kb on - strand at 57.520 kb on - strand at 57.520 kb on - strand at 57.691 kb on + strand, within puuA at 57.692 kb on - strand, within puuA at 57.692 kb on - strand, within puuA at 57.738 kb on - strand, within puuA at 57.807 kb on + strand, within puuA at 57.807 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.811 kb on + strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.812 kb on - strand, within puuA at 57.962 kb on + strand, within puuA at 57.963 kb on - strand, within puuA at 57.984 kb on - strand, within puuA at 58.043 kb on + strand, within puuA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 55,188 - -2.2 55,194 - -0.5 55,194 - +0.0 55,194 - +0.4 55,194 - +0.8 55,194 - +0.0 55,194 - +0.1 55,196 - +0.2 55,196 - +0.1 55,196 - -0.4 55,196 - -1.1 55,401 - MCAODC_15815 0.23 -0.9 55,486 + MCAODC_15815 0.58 -2.3 55,654 + +0.4 55,654 + -0.3 55,664 + -0.7 55,664 + -1.1 55,666 + +0.0 55,725 - +0.2 55,725 - +0.6 55,725 - -0.9 55,725 - +0.4 55,725 - +0.1 55,725 - +1.4 55,758 - +0.3 55,800 - +2.8 55,933 - puuP MCAODC_15820 0.15 +0.4 56,011 - puuP MCAODC_15820 0.21 +2.3 56,032 + puuP MCAODC_15820 0.22 -1.2 56,159 + puuP MCAODC_15820 0.31 +1.7 56,159 + puuP MCAODC_15820 0.31 -1.0 56,160 - puuP MCAODC_15820 0.32 +0.0 56,261 + puuP MCAODC_15820 0.39 +0.5 56,331 + puuP MCAODC_15820 0.44 +0.6 56,366 + puuP MCAODC_15820 0.46 +0.4 56,366 + puuP MCAODC_15820 0.46 -1.9 56,367 - puuP MCAODC_15820 0.46 -2.2 56,517 + puuP MCAODC_15820 0.57 -0.5 56,560 - puuP MCAODC_15820 0.60 +0.9 56,586 + puuP MCAODC_15820 0.62 +0.6 56,617 + puuP MCAODC_15820 0.65 -1.0 56,628 + puuP MCAODC_15820 0.65 +0.8 56,808 + puuP MCAODC_15820 0.78 +1.1 56,844 - puuP MCAODC_15820 0.81 +0.0 56,847 + puuP MCAODC_15820 0.81 -3.2 56,848 - puuP MCAODC_15820 0.81 +0.4 56,969 - puuP MCAODC_15820 0.90 -0.5 57,009 - -0.3 57,108 + -0.6 57,129 - +1.5 57,132 - -0.1 57,251 - +0.2 57,520 - -0.9 57,520 - -0.6 57,520 - +0.1 57,691 + puuA MCAODC_15825 0.20 -0.8 57,692 - puuA MCAODC_15825 0.20 -0.3 57,692 - puuA MCAODC_15825 0.20 +0.3 57,738 - puuA MCAODC_15825 0.23 +1.9 57,807 + puuA MCAODC_15825 0.28 +0.6 57,807 + puuA MCAODC_15825 0.28 -0.7 57,811 + puuA MCAODC_15825 0.28 -1.5 57,811 + puuA MCAODC_15825 0.28 -1.9 57,811 + puuA MCAODC_15825 0.28 -0.7 57,811 + puuA MCAODC_15825 0.28 +0.2 57,811 + puuA MCAODC_15825 0.28 +0.7 57,811 + puuA MCAODC_15825 0.28 +1.1 57,811 + puuA MCAODC_15825 0.28 -1.2 57,811 + puuA MCAODC_15825 0.28 +2.5 57,811 + puuA MCAODC_15825 0.28 +0.2 57,812 - puuA MCAODC_15825 0.28 -1.0 57,812 - puuA MCAODC_15825 0.28 +0.5 57,812 - puuA MCAODC_15825 0.28 +0.3 57,812 - puuA MCAODC_15825 0.28 -0.4 57,812 - puuA MCAODC_15825 0.28 +0.2 57,812 - puuA MCAODC_15825 0.28 +1.0 57,812 - puuA MCAODC_15825 0.28 -0.8 57,812 - puuA MCAODC_15825 0.28 +0.9 57,962 + puuA MCAODC_15825 0.39 +0.5 57,963 - puuA MCAODC_15825 0.39 -0.0 57,984 - puuA MCAODC_15825 0.40 -0.1 58,043 + puuA MCAODC_15825 0.45 +0.2
Or see this region's nucleotide sequence