Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaB and guaA are separated by 68 nucleotides guaA and yfgI are separated by 92 nucleotides yfgI and yfgH are separated by 15 nucleotides
MCAODC_12290: guaB - IMP dehydrogenase, at 2,434,753 to 2,436,219
guaB
MCAODC_12295: guaA - glutamine-hydrolyzing GMP synthase, at 2,436,288 to 2,437,865
guaA
MCAODC_12300: yfgI - Uncharacterized protein YfgI, at 2,437,958 to 2,438,497
yfgI
MCAODC_12305: yfgH - Uncharacterized lipoprotein YfgH, at 2,438,513 to 2,439,028
yfgH
Position (kb)
2436
2437
2438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2435.554 kb on + strand, within guaB at 2435.691 kb on - strand, within guaB at 2435.831 kb on + strand, within guaB at 2436.014 kb on + strand, within guaB at 2436.015 kb on - strand, within guaB at 2436.021 kb on + strand, within guaB at 2436.021 kb on + strand, within guaB at 2436.021 kb on + strand, within guaB at 2436.096 kb on + strand at 2436.096 kb on + strand at 2436.114 kb on + strand at 2436.117 kb on + strand at 2436.117 kb on + strand at 2436.131 kb on + strand at 2436.135 kb on + strand at 2436.183 kb on + strand at 2436.186 kb on + strand at 2436.186 kb on + strand at 2436.251 kb on + strand at 2436.251 kb on + strand at 2436.253 kb on + strand at 2436.253 kb on + strand at 2436.256 kb on + strand at 2436.256 kb on + strand at 2436.256 kb on + strand at 2436.305 kb on + strand at 2436.473 kb on + strand, within guaA at 2436.634 kb on + strand, within guaA at 2436.820 kb on + strand, within guaA at 2437.136 kb on + strand, within guaA at 2437.608 kb on + strand, within guaA at 2437.648 kb on + strand, within guaA at 2437.679 kb on + strand, within guaA at 2437.728 kb on - strand at 2437.821 kb on + strand at 2437.933 kb on - strand at 2437.933 kb on - strand at 2437.950 kb on - strand at 2438.260 kb on + strand, within yfgI at 2438.459 kb on + strand at 2438.469 kb on - strand at 2438.512 kb on - strand at 2438.536 kb on + strand at 2438.563 kb on - strand at 2438.575 kb on + strand, within yfgH at 2438.575 kb on + strand, within yfgH at 2438.597 kb on - strand, within yfgH at 2438.624 kb on - strand, within yfgH at 2438.629 kb on + strand, within yfgH at 2438.629 kb on + strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.630 kb on - strand, within yfgH at 2438.631 kb on + strand, within yfgH at 2438.631 kb on + strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.632 kb on - strand, within yfgH at 2438.650 kb on + strand, within yfgH at 2438.652 kb on + strand, within yfgH at 2438.652 kb on + strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.653 kb on - strand, within yfgH at 2438.676 kb on - strand, within yfgH at 2438.676 kb on - strand, within yfgH at 2438.695 kb on + strand, within yfgH at 2438.696 kb on - strand, within yfgH
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 2,435,554 + guaB MCAODC_12290 0.55 -1.8 2,435,691 - guaB MCAODC_12290 0.64 -1.2 2,435,831 + guaB MCAODC_12290 0.73 +0.1 2,436,014 + guaB MCAODC_12290 0.86 +0.7 2,436,015 - guaB MCAODC_12290 0.86 +0.8 2,436,021 + guaB MCAODC_12290 0.86 +2.6 2,436,021 + guaB MCAODC_12290 0.86 -1.4 2,436,021 + guaB MCAODC_12290 0.86 -0.9 2,436,096 + -0.9 2,436,096 + +0.2 2,436,114 + -1.1 2,436,117 + -0.6 2,436,117 + -1.0 2,436,131 + -1.7 2,436,135 + -0.9 2,436,183 + -1.5 2,436,186 + +0.4 2,436,186 + +0.4 2,436,251 + -0.8 2,436,251 + +1.4 2,436,253 + +1.5 2,436,253 + +0.4 2,436,256 + -0.4 2,436,256 + +1.1 2,436,256 + +0.8 2,436,305 + +1.0 2,436,473 + guaA MCAODC_12295 0.12 -0.9 2,436,634 + guaA MCAODC_12295 0.22 +1.6 2,436,820 + guaA MCAODC_12295 0.34 -0.9 2,437,136 + guaA MCAODC_12295 0.54 +1.0 2,437,608 + guaA MCAODC_12295 0.84 -0.6 2,437,648 + guaA MCAODC_12295 0.86 +1.0 2,437,679 + guaA MCAODC_12295 0.88 +1.3 2,437,728 - -0.7 2,437,821 + +0.4 2,437,933 - -1.7 2,437,933 - +0.5 2,437,950 - -1.0 2,438,260 + yfgI MCAODC_12300 0.56 -0.4 2,438,459 + -0.8 2,438,469 - +0.0 2,438,512 - +0.8 2,438,536 + -0.2 2,438,563 - +1.0 2,438,575 + yfgH MCAODC_12305 0.12 +0.4 2,438,575 + yfgH MCAODC_12305 0.12 +0.5 2,438,597 - yfgH MCAODC_12305 0.16 +0.1 2,438,624 - yfgH MCAODC_12305 0.22 -0.1 2,438,629 + yfgH MCAODC_12305 0.22 +0.3 2,438,629 + yfgH MCAODC_12305 0.22 -0.2 2,438,630 - yfgH MCAODC_12305 0.23 -3.1 2,438,630 - yfgH MCAODC_12305 0.23 +0.6 2,438,630 - yfgH MCAODC_12305 0.23 +0.1 2,438,630 - yfgH MCAODC_12305 0.23 -1.2 2,438,630 - yfgH MCAODC_12305 0.23 +1.5 2,438,631 + yfgH MCAODC_12305 0.23 +0.3 2,438,631 + yfgH MCAODC_12305 0.23 -0.2 2,438,632 - yfgH MCAODC_12305 0.23 -1.2 2,438,632 - yfgH MCAODC_12305 0.23 -0.6 2,438,632 - yfgH MCAODC_12305 0.23 +0.7 2,438,650 + yfgH MCAODC_12305 0.27 +1.2 2,438,652 + yfgH MCAODC_12305 0.27 +0.3 2,438,652 + yfgH MCAODC_12305 0.27 -0.4 2,438,653 - yfgH MCAODC_12305 0.27 -0.8 2,438,653 - yfgH MCAODC_12305 0.27 -0.5 2,438,653 - yfgH MCAODC_12305 0.27 -0.0 2,438,653 - yfgH MCAODC_12305 0.27 +0.3 2,438,653 - yfgH MCAODC_12305 0.27 +0.5 2,438,676 - yfgH MCAODC_12305 0.32 +0.1 2,438,676 - yfgH MCAODC_12305 0.32 +1.6 2,438,695 + yfgH MCAODC_12305 0.35 +1.0 2,438,696 - yfgH MCAODC_12305 0.35 +0.7
Or see this region's nucleotide sequence