Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recO and pdxJ are separated by 11 nucleotides pdxJ and acpS overlap by 1 nucleotides acpS and shoB are separated by 421 nucleotides shoB and yfhL are separated by 193 nucleotides
MCAODC_11950: recO - DNA repair protein RecO, at 2,362,112 to 2,362,840
recO
MCAODC_11955: pdxJ - pyridoxine 5'-phosphate synthase, at 2,362,852 to 2,363,583
pdxJ
MCAODC_11960: acpS - Holo-[acyl-carrier-protein] synthase, at 2,363,583 to 2,363,963
acpS
MCAODC_11970: shoB - Small toxic protein ShoB, at 2,364,385 to 2,364,465
shoB
MCAODC_11975: yfhL - 4Fe-4S dicluster ferredoxin YfhL, at 2,364,659 to 2,364,919
yfhL
Position (kb)
2363
2364 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2362.585 kb on - strand, within recO at 2362.601 kb on + strand, within recO at 2362.674 kb on + strand, within recO at 2362.675 kb on - strand, within recO at 2362.804 kb on + strand at 2362.832 kb on + strand at 2362.832 kb on + strand at 2362.862 kb on + strand at 2362.988 kb on + strand, within pdxJ at 2363.153 kb on + strand, within pdxJ at 2363.983 kb on - strand at 2363.984 kb on + strand at 2364.023 kb on - strand at 2364.095 kb on - strand at 2364.188 kb on + strand at 2364.188 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.339 kb on + strand at 2364.340 kb on - strand at 2364.340 kb on - strand at 2364.340 kb on - strand at 2364.441 kb on - strand, within shoB at 2364.461 kb on + strand at 2364.482 kb on + strand at 2364.521 kb on - strand at 2364.531 kb on - strand at 2364.531 kb on - strand at 2364.531 kb on - strand at 2364.531 kb on - strand at 2364.531 kb on - strand at 2364.546 kb on + strand at 2364.603 kb on - strand at 2364.633 kb on + strand at 2364.641 kb on + strand at 2364.642 kb on - strand at 2364.642 kb on - strand at 2364.742 kb on + strand, within yfhL at 2364.743 kb on - strand, within yfhL at 2364.745 kb on + strand, within yfhL at 2364.812 kb on - strand, within yfhL at 2364.812 kb on - strand, within yfhL at 2364.812 kb on - strand, within yfhL at 2364.820 kb on + strand, within yfhL at 2364.828 kb on + strand, within yfhL at 2364.832 kb on + strand, within yfhL at 2364.832 kb on + strand, within yfhL at 2364.833 kb on - strand, within yfhL at 2364.833 kb on - strand, within yfhL at 2364.833 kb on - strand, within yfhL at 2364.880 kb on + strand, within yfhL at 2364.958 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 2,362,585 - recO MCAODC_11950 0.65 +1.1 2,362,601 + recO MCAODC_11950 0.67 -0.1 2,362,674 + recO MCAODC_11950 0.77 -0.9 2,362,675 - recO MCAODC_11950 0.77 -0.2 2,362,804 + -0.2 2,362,832 + -1.6 2,362,832 + +1.0 2,362,862 + -2.0 2,362,988 + pdxJ MCAODC_11955 0.19 +2.0 2,363,153 + pdxJ MCAODC_11955 0.41 -1.2 2,363,983 - -0.7 2,363,984 + -0.3 2,364,023 - -0.8 2,364,095 - -0.2 2,364,188 + -0.2 2,364,188 + +1.7 2,364,339 + -0.9 2,364,339 + -2.0 2,364,339 + -0.9 2,364,339 + +0.9 2,364,339 + +0.4 2,364,339 + -0.2 2,364,339 + +0.4 2,364,339 + -0.0 2,364,340 - -0.2 2,364,340 - +0.4 2,364,340 - +0.6 2,364,441 - shoB MCAODC_11970 0.69 -1.0 2,364,461 + -0.4 2,364,482 + +1.1 2,364,521 - +1.2 2,364,531 - +0.2 2,364,531 - +2.6 2,364,531 - -0.1 2,364,531 - -1.0 2,364,531 - -1.2 2,364,546 + -0.2 2,364,603 - -0.1 2,364,633 + +0.5 2,364,641 + +0.8 2,364,642 - +1.0 2,364,642 - -0.2 2,364,742 + yfhL MCAODC_11975 0.32 -1.1 2,364,743 - yfhL MCAODC_11975 0.32 +0.1 2,364,745 + yfhL MCAODC_11975 0.33 -0.8 2,364,812 - yfhL MCAODC_11975 0.59 +0.6 2,364,812 - yfhL MCAODC_11975 0.59 -0.3 2,364,812 - yfhL MCAODC_11975 0.59 -2.3 2,364,820 + yfhL MCAODC_11975 0.62 -1.7 2,364,828 + yfhL MCAODC_11975 0.65 +0.8 2,364,832 + yfhL MCAODC_11975 0.66 +3.0 2,364,832 + yfhL MCAODC_11975 0.66 +0.3 2,364,833 - yfhL MCAODC_11975 0.67 -0.3 2,364,833 - yfhL MCAODC_11975 0.67 +0.3 2,364,833 - yfhL MCAODC_11975 0.67 -0.3 2,364,880 + yfhL MCAODC_11975 0.85 +0.1 2,364,958 - -1.6
Or see this region's nucleotide sequence