Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_00160 and MCAODC_00165 are separated by 127 nucleotides MCAODC_00165 and tnp-IS3 are separated by 25 nucleotides tnp-IS3 and tnp-IS3 overlap by 1 nucleotides tnp-IS3 and xerD are separated by 462 nucleotides
MCAODC_00160: MCAODC_00160 - Repressor protein cI, at 10,622 to 11,335
_00160
MCAODC_00165: MCAODC_00165 - hypothetical protein, at 11,463 to 11,846
_00165
MCAODC_00170: tnp-IS3 - IS3 family transposase, at 11,872 to 12,198
tnp-IS3
MCAODC_00175: tnp-IS3 - IS3 family transposase, at 12,198 to 13,085
tnp-IS3
MCAODC_00180: xerD - Site-specific recombinase XerD, at 13,548 to 14,522
xerD
Position (kb) 12
13
14 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 11.232 kb on + strand, within MCAODC_00160 at 11.233 kb on - strand, within MCAODC_00160 at 11.233 kb on - strand, within MCAODC_00160 at 11.241 kb on + strand, within MCAODC_00160 at 11.242 kb on - strand, within MCAODC_00160 at 11.242 kb on - strand, within MCAODC_00160 at 11.299 kb on + strand at 11.305 kb on + strand at 11.306 kb on - strand at 11.336 kb on + strand at 11.344 kb on - strand at 11.386 kb on + strand at 11.423 kb on + strand at 11.462 kb on + strand at 11.462 kb on + strand at 11.462 kb on + strand at 11.462 kb on + strand at 11.483 kb on - strand at 11.483 kb on - strand at 11.519 kb on + strand, within MCAODC_00165 at 11.575 kb on + strand, within MCAODC_00165 at 11.604 kb on - strand, within MCAODC_00165 at 11.631 kb on - strand, within MCAODC_00165 at 11.651 kb on + strand, within MCAODC_00165 at 11.658 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.664 kb on + strand, within MCAODC_00165 at 11.665 kb on - strand, within MCAODC_00165 at 11.665 kb on - strand, within MCAODC_00165 at 11.665 kb on - strand, within MCAODC_00165 at 11.665 kb on - strand, within MCAODC_00165 at 11.720 kb on + strand, within MCAODC_00165 at 11.720 kb on + strand, within MCAODC_00165 at 11.720 kb on + strand, within MCAODC_00165 at 11.720 kb on + strand, within MCAODC_00165 at 11.760 kb on + strand, within MCAODC_00165 at 11.762 kb on + strand, within MCAODC_00165 at 11.762 kb on + strand, within MCAODC_00165 at 11.762 kb on + strand, within MCAODC_00165 at 11.792 kb on - strand, within MCAODC_00165 at 11.792 kb on - strand, within MCAODC_00165 at 13.095 kb on + strand at 13.095 kb on + strand at 13.234 kb on + strand at 13.363 kb on - strand at 13.370 kb on - strand at 13.421 kb on - strand at 13.525 kb on - strand at 13.525 kb on - strand at 13.525 kb on - strand at 13.525 kb on - strand at 13.528 kb on + strand at 13.528 kb on + strand at 13.528 kb on + strand at 13.529 kb on - strand at 13.529 kb on - strand at 13.529 kb on - strand at 13.642 kb on + strand at 13.642 kb on + strand at 13.642 kb on + strand at 13.643 kb on - strand at 13.643 kb on - strand at 13.653 kb on - strand, within xerD at 13.653 kb on - strand, within xerD at 13.764 kb on + strand, within xerD at 13.814 kb on + strand, within xerD at 13.814 kb on + strand, within xerD at 13.829 kb on - strand, within xerD at 13.877 kb on + strand, within xerD at 13.999 kb on + strand, within xerD at 14.000 kb on - strand, within xerD at 14.000 kb on - strand, within xerD at 14.038 kb on + strand, within xerD at 14.038 kb on + strand, within xerD at 14.038 kb on + strand, within xerD at 14.038 kb on + strand, within xerD at 14.038 kb on + strand, within xerD at 14.038 kb on + strand, within xerD at 14.039 kb on - strand, within xerD at 14.039 kb on - strand, within xerD at 14.042 kb on + strand, within xerD at 14.043 kb on - strand, within xerD at 14.043 kb on - strand, within xerD at 14.043 kb on - strand, within xerD at 14.082 kb on - strand, within xerD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 11,232 + MCAODC_00160 0.85 +0.3 11,233 - MCAODC_00160 0.86 +0.6 11,233 - MCAODC_00160 0.86 +0.6 11,241 + MCAODC_00160 0.87 -1.4 11,242 - MCAODC_00160 0.87 +0.8 11,242 - MCAODC_00160 0.87 +0.2 11,299 + +0.5 11,305 + +0.3 11,306 - -0.1 11,336 + +2.7 11,344 - -0.0 11,386 + +3.7 11,423 + -0.2 11,462 + +0.5 11,462 + -0.1 11,462 + -0.2 11,462 + +0.1 11,483 - -1.1 11,483 - -1.5 11,519 + MCAODC_00165 0.15 -0.1 11,575 + MCAODC_00165 0.29 -1.1 11,604 - MCAODC_00165 0.37 -1.5 11,631 - MCAODC_00165 0.44 +0.4 11,651 + MCAODC_00165 0.49 +1.4 11,658 + MCAODC_00165 0.51 +0.3 11,664 + MCAODC_00165 0.52 +0.8 11,664 + MCAODC_00165 0.52 -0.6 11,664 + MCAODC_00165 0.52 +2.8 11,664 + MCAODC_00165 0.52 +0.4 11,664 + MCAODC_00165 0.52 -0.1 11,664 + MCAODC_00165 0.52 -1.6 11,664 + MCAODC_00165 0.52 -2.3 11,665 - MCAODC_00165 0.53 -0.4 11,665 - MCAODC_00165 0.53 +1.1 11,665 - MCAODC_00165 0.53 +1.4 11,665 - MCAODC_00165 0.53 -0.3 11,720 + MCAODC_00165 0.67 -0.2 11,720 + MCAODC_00165 0.67 -0.5 11,720 + MCAODC_00165 0.67 -0.1 11,720 + MCAODC_00165 0.67 -0.3 11,760 + MCAODC_00165 0.77 +1.0 11,762 + MCAODC_00165 0.78 -0.3 11,762 + MCAODC_00165 0.78 -0.4 11,762 + MCAODC_00165 0.78 -0.1 11,792 - MCAODC_00165 0.86 +0.2 11,792 - MCAODC_00165 0.86 +0.2 13,095 + +1.4 13,095 + +1.2 13,234 + -0.8 13,363 - -1.9 13,370 - -0.8 13,421 - -0.7 13,525 - +1.1 13,525 - +0.5 13,525 - -0.6 13,525 - +0.7 13,528 + +0.4 13,528 + -0.1 13,528 + +0.9 13,529 - +0.2 13,529 - -0.2 13,529 - +0.1 13,642 + +0.5 13,642 + +1.7 13,642 + -0.4 13,643 - +0.0 13,643 - -0.5 13,653 - xerD MCAODC_00180 0.11 -0.4 13,653 - xerD MCAODC_00180 0.11 +0.2 13,764 + xerD MCAODC_00180 0.22 +0.7 13,814 + xerD MCAODC_00180 0.27 -1.2 13,814 + xerD MCAODC_00180 0.27 -0.3 13,829 - xerD MCAODC_00180 0.29 -0.7 13,877 + xerD MCAODC_00180 0.34 +0.6 13,999 + xerD MCAODC_00180 0.46 -1.0 14,000 - xerD MCAODC_00180 0.46 +0.9 14,000 - xerD MCAODC_00180 0.46 +2.4 14,038 + xerD MCAODC_00180 0.50 +0.2 14,038 + xerD MCAODC_00180 0.50 +0.7 14,038 + xerD MCAODC_00180 0.50 -0.9 14,038 + xerD MCAODC_00180 0.50 +0.1 14,038 + xerD MCAODC_00180 0.50 +0.3 14,038 + xerD MCAODC_00180 0.50 -2.3 14,039 - xerD MCAODC_00180 0.50 -1.0 14,039 - xerD MCAODC_00180 0.50 +2.0 14,042 + xerD MCAODC_00180 0.51 +0.1 14,043 - xerD MCAODC_00180 0.51 -1.3 14,043 - xerD MCAODC_00180 0.51 -1.3 14,043 - xerD MCAODC_00180 0.51 +0.1 14,082 - xerD MCAODC_00180 0.55 -0.1
Or see this region's nucleotide sequence