Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00025 and A4249_RS00030 overlap by 17 nucleotides A4249_RS00030 and A4249_RS00035 are separated by 5 nucleotides A4249_RS00035 and A4249_RS00040 are separated by 36 nucleotides A4249_RS00040 and A4249_RS00045 are separated by 79 nucleotides
A4249_RS00025: A4249_RS00025 - retropepsin-like aspartic protease, at 4,734 to 5,681
_RS00025
A4249_RS00030: A4249_RS00030 - hypothetical protein, at 5,665 to 6,123
_RS00030
A4249_RS00035: A4249_RS00035 - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 6,129 to 7,766
_RS00035
A4249_RS00040: A4249_RS00040 - neutral zinc metallopeptidase, at 7,803 to 8,654
_RS00040
A4249_RS00045: A4249_RS00045 - hypothetical protein, at 8,734 to 9,123
_RS00045
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-1
0
1 at 5.795 kb on + strand, within A4249_RS00030 at 5.795 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.059 kb on + strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.060 kb on - strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.077 kb on + strand, within A4249_RS00030 at 6.078 kb on - strand at 6.078 kb on - strand at 6.078 kb on - strand at 6.089 kb on + strand at 6.214 kb on + strand at 6.214 kb on + strand at 6.224 kb on - strand at 6.224 kb on - strand at 6.433 kb on + strand, within A4249_RS00035 at 6.469 kb on + strand, within A4249_RS00035 at 6.469 kb on + strand, within A4249_RS00035 at 6.469 kb on + strand, within A4249_RS00035 at 6.470 kb on - strand, within A4249_RS00035 at 6.470 kb on - strand, within A4249_RS00035 at 6.470 kb on - strand, within A4249_RS00035 at 6.555 kb on + strand, within A4249_RS00035 at 6.556 kb on - strand, within A4249_RS00035 at 6.775 kb on + strand, within A4249_RS00035 at 6.775 kb on + strand, within A4249_RS00035 at 6.775 kb on + strand, within A4249_RS00035 at 6.776 kb on - strand, within A4249_RS00035 at 6.776 kb on - strand, within A4249_RS00035 at 6.776 kb on - strand, within A4249_RS00035 at 6.880 kb on + strand, within A4249_RS00035 at 6.880 kb on + strand, within A4249_RS00035 at 7.051 kb on + strand, within A4249_RS00035 at 7.051 kb on + strand, within A4249_RS00035 at 7.051 kb on + strand, within A4249_RS00035 at 7.052 kb on - strand, within A4249_RS00035 at 7.052 kb on - strand, within A4249_RS00035 at 7.052 kb on - strand, within A4249_RS00035 at 7.146 kb on - strand, within A4249_RS00035 at 7.146 kb on - strand, within A4249_RS00035 at 7.147 kb on + strand, within A4249_RS00035 at 7.147 kb on + strand, within A4249_RS00035 at 7.147 kb on + strand, within A4249_RS00035 at 7.147 kb on + strand, within A4249_RS00035 at 7.147 kb on + strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.148 kb on - strand, within A4249_RS00035 at 7.159 kb on + strand, within A4249_RS00035 at 7.160 kb on - strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.303 kb on + strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.304 kb on - strand, within A4249_RS00035 at 7.370 kb on + strand, within A4249_RS00035 at 7.371 kb on - strand, within A4249_RS00035 at 7.415 kb on + strand, within A4249_RS00035 at 7.561 kb on + strand, within A4249_RS00035 at 7.697 kb on - strand at 7.970 kb on + strand, within A4249_RS00040 at 7.970 kb on + strand, within A4249_RS00040 at 7.970 kb on + strand, within A4249_RS00040 at 7.970 kb on + strand, within A4249_RS00040 at 7.970 kb on + strand, within A4249_RS00040 at 7.971 kb on - strand, within A4249_RS00040 at 8.050 kb on + strand, within A4249_RS00040 at 8.050 kb on + strand, within A4249_RS00040 at 8.051 kb on - strand, within A4249_RS00040 at 8.051 kb on - strand, within A4249_RS00040 at 8.083 kb on + strand, within A4249_RS00040 at 8.084 kb on - strand, within A4249_RS00040 at 8.084 kb on - strand, within A4249_RS00040 at 8.084 kb on - strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.191 kb on + strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.192 kb on - strand, within A4249_RS00040 at 8.263 kb on + strand, within A4249_RS00040 at 8.263 kb on + strand, within A4249_RS00040 at 8.263 kb on + strand, within A4249_RS00040 at 8.264 kb on - strand, within A4249_RS00040 at 8.287 kb on + strand, within A4249_RS00040 at 8.287 kb on + strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.288 kb on - strand, within A4249_RS00040 at 8.315 kb on - strand, within A4249_RS00040 at 8.315 kb on - strand, within A4249_RS00040 at 8.344 kb on + strand, within A4249_RS00040 at 8.345 kb on - strand, within A4249_RS00040 at 8.368 kb on + strand, within A4249_RS00040 at 8.403 kb on - strand, within A4249_RS00040 at 8.403 kb on - strand, within A4249_RS00040 at 8.403 kb on - strand, within A4249_RS00040 at 8.403 kb on - strand, within A4249_RS00040 at 8.542 kb on + strand, within A4249_RS00040 at 8.543 kb on - strand, within A4249_RS00040 at 8.659 kb on + strand at 8.659 kb on + strand at 8.681 kb on + strand at 8.682 kb on - strand at 8.682 kb on - strand at 8.682 kb on - strand at 8.686 kb on + strand at 8.686 kb on + strand at 8.686 kb on + strand at 8.686 kb on + strand at 8.687 kb on - strand at 8.687 kb on - strand at 8.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 5,795 + A4249_RS00030 0.28 +0.5 5,795 + A4249_RS00030 0.28 -0.1 6,059 + A4249_RS00030 0.86 +1.1 6,059 + A4249_RS00030 0.86 +0.2 6,059 + A4249_RS00030 0.86 -0.6 6,059 + A4249_RS00030 0.86 -0.1 6,059 + A4249_RS00030 0.86 -0.2 6,059 + A4249_RS00030 0.86 -0.1 6,059 + A4249_RS00030 0.86 +0.8 6,059 + A4249_RS00030 0.86 +0.2 6,059 + A4249_RS00030 0.86 +0.2 6,060 - A4249_RS00030 0.86 +1.3 6,060 - A4249_RS00030 0.86 +0.1 6,060 - A4249_RS00030 0.86 +0.7 6,060 - A4249_RS00030 0.86 -0.5 6,060 - A4249_RS00030 0.86 -0.8 6,060 - A4249_RS00030 0.86 -0.0 6,077 + A4249_RS00030 0.90 -0.0 6,077 + A4249_RS00030 0.90 +0.0 6,077 + A4249_RS00030 0.90 +0.5 6,077 + A4249_RS00030 0.90 -0.6 6,077 + A4249_RS00030 0.90 -0.6 6,077 + A4249_RS00030 0.90 +0.5 6,078 - +0.7 6,078 - +1.0 6,078 - +0.8 6,089 + -0.2 6,214 + -0.9 6,214 + -0.2 6,224 - -0.1 6,224 - -0.3 6,433 + A4249_RS00035 0.19 +0.3 6,469 + A4249_RS00035 0.21 +0.3 6,469 + A4249_RS00035 0.21 +0.2 6,469 + A4249_RS00035 0.21 +0.4 6,470 - A4249_RS00035 0.21 -0.2 6,470 - A4249_RS00035 0.21 +0.6 6,470 - A4249_RS00035 0.21 +0.4 6,555 + A4249_RS00035 0.26 +1.0 6,556 - A4249_RS00035 0.26 +0.5 6,775 + A4249_RS00035 0.39 -0.3 6,775 + A4249_RS00035 0.39 +0.0 6,775 + A4249_RS00035 0.39 -0.0 6,776 - A4249_RS00035 0.39 -0.8 6,776 - A4249_RS00035 0.39 +0.2 6,776 - A4249_RS00035 0.39 +0.6 6,880 + A4249_RS00035 0.46 +0.3 6,880 + A4249_RS00035 0.46 +0.5 7,051 + A4249_RS00035 0.56 +0.5 7,051 + A4249_RS00035 0.56 +0.7 7,051 + A4249_RS00035 0.56 -0.0 7,052 - A4249_RS00035 0.56 +0.7 7,052 - A4249_RS00035 0.56 +0.1 7,052 - A4249_RS00035 0.56 -0.1 7,146 - A4249_RS00035 0.62 +0.1 7,146 - A4249_RS00035 0.62 +0.0 7,147 + A4249_RS00035 0.62 -0.3 7,147 + A4249_RS00035 0.62 +0.2 7,147 + A4249_RS00035 0.62 +1.0 7,147 + A4249_RS00035 0.62 +0.4 7,147 + A4249_RS00035 0.62 +0.2 7,148 - A4249_RS00035 0.62 -0.4 7,148 - A4249_RS00035 0.62 +0.6 7,148 - A4249_RS00035 0.62 -0.3 7,148 - A4249_RS00035 0.62 -0.4 7,148 - A4249_RS00035 0.62 +1.2 7,148 - A4249_RS00035 0.62 -0.1 7,159 + A4249_RS00035 0.63 -0.5 7,160 - A4249_RS00035 0.63 +0.3 7,303 + A4249_RS00035 0.72 +0.5 7,303 + A4249_RS00035 0.72 -0.2 7,303 + A4249_RS00035 0.72 -1.6 7,303 + A4249_RS00035 0.72 +0.5 7,303 + A4249_RS00035 0.72 -0.1 7,303 + A4249_RS00035 0.72 -0.5 7,303 + A4249_RS00035 0.72 +0.0 7,303 + A4249_RS00035 0.72 +0.3 7,303 + A4249_RS00035 0.72 +0.2 7,303 + A4249_RS00035 0.72 +0.1 7,303 + A4249_RS00035 0.72 +0.2 7,304 - A4249_RS00035 0.72 +0.6 7,304 - A4249_RS00035 0.72 +0.1 7,304 - A4249_RS00035 0.72 +0.3 7,304 - A4249_RS00035 0.72 -0.6 7,304 - A4249_RS00035 0.72 -0.4 7,304 - A4249_RS00035 0.72 -0.5 7,304 - A4249_RS00035 0.72 -1.0 7,304 - A4249_RS00035 0.72 +0.5 7,304 - A4249_RS00035 0.72 +0.0 7,304 - A4249_RS00035 0.72 +0.4 7,304 - A4249_RS00035 0.72 +0.1 7,304 - A4249_RS00035 0.72 +0.5 7,304 - A4249_RS00035 0.72 +0.0 7,370 + A4249_RS00035 0.76 +0.1 7,371 - A4249_RS00035 0.76 +0.2 7,415 + A4249_RS00035 0.79 +0.2 7,561 + A4249_RS00035 0.87 -0.4 7,697 - +0.3 7,970 + A4249_RS00040 0.20 +0.4 7,970 + A4249_RS00040 0.20 +0.3 7,970 + A4249_RS00040 0.20 -0.1 7,970 + A4249_RS00040 0.20 -0.1 7,970 + A4249_RS00040 0.20 +0.3 7,971 - A4249_RS00040 0.20 -0.5 8,050 + A4249_RS00040 0.29 +0.6 8,050 + A4249_RS00040 0.29 +0.3 8,051 - A4249_RS00040 0.29 +0.3 8,051 - A4249_RS00040 0.29 -0.2 8,083 + A4249_RS00040 0.33 +0.8 8,084 - A4249_RS00040 0.33 -0.6 8,084 - A4249_RS00040 0.33 -0.1 8,084 - A4249_RS00040 0.33 +0.0 8,191 + A4249_RS00040 0.46 -0.8 8,191 + A4249_RS00040 0.46 +0.0 8,191 + A4249_RS00040 0.46 +0.8 8,191 + A4249_RS00040 0.46 -0.2 8,191 + A4249_RS00040 0.46 +0.6 8,191 + A4249_RS00040 0.46 +0.2 8,192 - A4249_RS00040 0.46 -0.1 8,192 - A4249_RS00040 0.46 -0.8 8,192 - A4249_RS00040 0.46 +1.2 8,192 - A4249_RS00040 0.46 +0.5 8,192 - A4249_RS00040 0.46 +1.2 8,192 - A4249_RS00040 0.46 -1.2 8,192 - A4249_RS00040 0.46 -0.1 8,192 - A4249_RS00040 0.46 +0.3 8,192 - A4249_RS00040 0.46 +0.6 8,192 - A4249_RS00040 0.46 +0.8 8,263 + A4249_RS00040 0.54 -0.5 8,263 + A4249_RS00040 0.54 +0.5 8,263 + A4249_RS00040 0.54 -0.3 8,264 - A4249_RS00040 0.54 +1.1 8,287 + A4249_RS00040 0.57 +0.8 8,287 + A4249_RS00040 0.57 +0.6 8,288 - A4249_RS00040 0.57 +0.6 8,288 - A4249_RS00040 0.57 +0.6 8,288 - A4249_RS00040 0.57 +0.2 8,288 - A4249_RS00040 0.57 -0.3 8,288 - A4249_RS00040 0.57 +1.7 8,288 - A4249_RS00040 0.57 -0.2 8,288 - A4249_RS00040 0.57 -1.7 8,288 - A4249_RS00040 0.57 -0.1 8,315 - A4249_RS00040 0.60 +0.9 8,315 - A4249_RS00040 0.60 +0.2 8,344 + A4249_RS00040 0.63 -0.6 8,345 - A4249_RS00040 0.64 +0.2 8,368 + A4249_RS00040 0.66 +0.4 8,403 - A4249_RS00040 0.70 -0.1 8,403 - A4249_RS00040 0.70 +0.0 8,403 - A4249_RS00040 0.70 -0.4 8,403 - A4249_RS00040 0.70 +0.2 8,542 + A4249_RS00040 0.87 +0.2 8,543 - A4249_RS00040 0.87 -0.8 8,659 + -0.3 8,659 + +0.4 8,681 + -0.4 8,682 - +0.8 8,682 - +0.7 8,682 - -0.1 8,686 + -0.4 8,686 + -1.4 8,686 + +0.6 8,686 + +0.3 8,687 - -0.0 8,687 - +0.8 8,687 - +0.3
Or see this region's nucleotide sequence