Strain Fitness in Escherichia coli ECRC101 around MCAODC_24020

Experiment: WM_RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrutC and rutD are separated by 50 nucleotidesrutD and rutE are separated by 9 nucleotidesrutE and rutF are separated by 10 nucleotidesrutF and rutG are separated by 20 nucleotides MCAODC_24010: rutC - pyrimidine utilization protein C, at 1,530,123 to 1,530,419 rutC MCAODC_24015: rutD - pyrimidine utilization protein D, at 1,530,470 to 1,531,270 rutD MCAODC_24020: rutE - malonic semialdehyde reductase, at 1,531,280 to 1,531,870 rutE MCAODC_24025: rutF - pyrimidine utilization flavin reductase protein F, at 1,531,881 to 1,532,375 rutF MCAODC_24030: rutG - pyrimidine utilization transport protein G, at 1,532,396 to 1,533,724 rutG Position (kb) 1531 1532Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1530.307 kb on + strand, within rutCat 1530.360 kb on - strand, within rutCat 1530.411 kb on - strandat 1530.451 kb on - strandat 1530.497 kb on + strandat 1530.514 kb on - strandat 1530.650 kb on + strand, within rutDat 1530.656 kb on - strand, within rutDat 1530.819 kb on + strand, within rutDat 1530.820 kb on - strand, within rutDat 1530.862 kb on - strand, within rutDat 1530.866 kb on + strand, within rutDat 1531.003 kb on - strand, within rutDat 1531.016 kb on - strand, within rutDat 1531.211 kb on - strandat 1531.245 kb on + strandat 1531.245 kb on + strandat 1531.246 kb on - strandat 1531.246 kb on - strandat 1531.246 kb on - strandat 1531.246 kb on - strandat 1531.279 kb on + strandat 1531.280 kb on - strandat 1531.280 kb on - strandat 1531.421 kb on - strand, within rutEat 1531.421 kb on - strand, within rutEat 1531.421 kb on - strand, within rutEat 1531.421 kb on - strand, within rutEat 1531.421 kb on - strand, within rutEat 1531.438 kb on + strand, within rutEat 1531.438 kb on + strand, within rutEat 1531.740 kb on + strand, within rutEat 1531.741 kb on - strand, within rutEat 1531.789 kb on + strand, within rutEat 1531.789 kb on + strand, within rutEat 1531.792 kb on + strand, within rutEat 1531.868 kb on + strandat 1531.869 kb on - strandat 1531.881 kb on - strandat 1531.881 kb on - strandat 1531.881 kb on - strandat 1531.881 kb on - strandat 1532.092 kb on + strand, within rutFat 1532.093 kb on - strand, within rutFat 1532.096 kb on + strand, within rutFat 1532.096 kb on + strand, within rutFat 1532.346 kb on + strandat 1532.427 kb on + strandat 1532.497 kb on - strandat 1532.542 kb on - strand, within rutGat 1532.583 kb on + strand, within rutGat 1532.584 kb on - strand, within rutGat 1532.584 kb on - strand, within rutGat 1532.605 kb on - strand, within rutGat 1532.643 kb on - strand, within rutGat 1532.643 kb on - strand, within rutGat 1532.762 kb on + strand, within rutGat 1532.763 kb on - strand, within rutGat 1532.764 kb on + strand, within rutG

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_RB68
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1,530,307 + rutC MCAODC_24010 0.62 +1.5
1,530,360 - rutC MCAODC_24010 0.80 -0.6
1,530,411 - +0.4
1,530,451 - +0.1
1,530,497 + +0.3
1,530,514 - -0.6
1,530,650 + rutD MCAODC_24015 0.22 +1.2
1,530,656 - rutD MCAODC_24015 0.23 +2.3
1,530,819 + rutD MCAODC_24015 0.44 -0.1
1,530,820 - rutD MCAODC_24015 0.44 +0.9
1,530,862 - rutD MCAODC_24015 0.49 +0.4
1,530,866 + rutD MCAODC_24015 0.49 -1.2
1,531,003 - rutD MCAODC_24015 0.67 +0.7
1,531,016 - rutD MCAODC_24015 0.68 +0.1
1,531,211 - -0.3
1,531,245 + +0.2
1,531,245 + -1.2
1,531,246 - +1.0
1,531,246 - +0.1
1,531,246 - +1.1
1,531,246 - -0.2
1,531,279 + -0.1
1,531,280 - -0.4
1,531,280 - -0.2
1,531,421 - rutE MCAODC_24020 0.24 +0.5
1,531,421 - rutE MCAODC_24020 0.24 +0.3
1,531,421 - rutE MCAODC_24020 0.24 -1.8
1,531,421 - rutE MCAODC_24020 0.24 -0.2
1,531,421 - rutE MCAODC_24020 0.24 -0.8
1,531,438 + rutE MCAODC_24020 0.27 -0.8
1,531,438 + rutE MCAODC_24020 0.27 +1.1
1,531,740 + rutE MCAODC_24020 0.78 +0.4
1,531,741 - rutE MCAODC_24020 0.78 -0.0
1,531,789 + rutE MCAODC_24020 0.86 +0.5
1,531,789 + rutE MCAODC_24020 0.86 +3.0
1,531,792 + rutE MCAODC_24020 0.87 +0.2
1,531,868 + +0.6
1,531,869 - -2.8
1,531,881 - +0.5
1,531,881 - +0.8
1,531,881 - -0.5
1,531,881 - +1.8
1,532,092 + rutF MCAODC_24025 0.43 +1.2
1,532,093 - rutF MCAODC_24025 0.43 -0.5
1,532,096 + rutF MCAODC_24025 0.43 +1.0
1,532,096 + rutF MCAODC_24025 0.43 -0.5
1,532,346 + +0.0
1,532,427 + +1.3
1,532,497 - -0.0
1,532,542 - rutG MCAODC_24030 0.11 +0.9
1,532,583 + rutG MCAODC_24030 0.14 -0.1
1,532,584 - rutG MCAODC_24030 0.14 -0.1
1,532,584 - rutG MCAODC_24030 0.14 +0.2
1,532,605 - rutG MCAODC_24030 0.16 +0.3
1,532,643 - rutG MCAODC_24030 0.19 +0.2
1,532,643 - rutG MCAODC_24030 0.19 +1.3
1,532,762 + rutG MCAODC_24030 0.28 -0.2
1,532,763 - rutG MCAODC_24030 0.28 -1.6
1,532,764 + rutG MCAODC_24030 0.28 +0.9

Or see this region's nucleotide sequence