Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgaB and pgaC overlap by 8 nucleotides pgaC and pgaD are separated by 1 nucleotides pgaD and phoH are separated by 49 nucleotides
MCAODC_23930: pgaB - poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB, at 1,510,294 to 1,512,312
pgaB
MCAODC_23935: pgaC - poly-beta-1,6-N-acetyl-D-glucosamine synthase, at 1,512,305 to 1,513,630
pgaC
MCAODC_23940: pgaD - poly-beta-1,6-N-acetyl-D-glucosamine biosynthesis protein PgaD, at 1,513,632 to 1,514,045
pgaD
MCAODC_23945: phoH - phosphate starvation-inducible protein PhoH, at 1,514,095 to 1,515,159
phoH
Position (kb)
1512
1513
1514 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1511.459 kb on + strand, within pgaB at 1511.459 kb on + strand, within pgaB at 1511.538 kb on - strand, within pgaB at 1511.639 kb on + strand, within pgaB at 1511.639 kb on + strand, within pgaB at 1511.678 kb on + strand, within pgaB at 1511.710 kb on + strand, within pgaB at 1512.337 kb on - strand at 1512.360 kb on + strand at 1512.524 kb on - strand, within pgaC at 1512.532 kb on + strand, within pgaC at 1512.827 kb on + strand, within pgaC at 1512.829 kb on + strand, within pgaC at 1512.829 kb on + strand, within pgaC at 1512.848 kb on + strand, within pgaC at 1512.901 kb on + strand, within pgaC at 1512.984 kb on + strand, within pgaC at 1513.081 kb on + strand, within pgaC at 1513.082 kb on - strand, within pgaC at 1513.143 kb on + strand, within pgaC at 1513.280 kb on + strand, within pgaC at 1513.378 kb on + strand, within pgaC at 1513.631 kb on + strand at 1513.686 kb on + strand, within pgaD at 1513.687 kb on - strand, within pgaD at 1513.928 kb on - strand, within pgaD at 1513.934 kb on - strand, within pgaD at 1514.005 kb on + strand at 1514.005 kb on + strand at 1514.087 kb on + strand at 1514.090 kb on + strand at 1514.091 kb on - strand at 1514.091 kb on - strand at 1514.096 kb on + strand at 1514.096 kb on + strand at 1514.097 kb on - strand at 1514.103 kb on - strand at 1514.103 kb on - strand at 1514.115 kb on + strand at 1514.116 kb on - strand at 1514.209 kb on + strand, within phoH at 1514.210 kb on - strand, within phoH at 1514.364 kb on + strand, within phoH at 1514.378 kb on + strand, within phoH at 1514.379 kb on - strand, within phoH at 1514.407 kb on + strand, within phoH at 1514.483 kb on + strand, within phoH at 1514.515 kb on + strand, within phoH at 1514.580 kb on + strand, within phoH at 1514.580 kb on + strand, within phoH at 1514.581 kb on - strand, within phoH at 1514.581 kb on - strand, within phoH at 1514.584 kb on + strand, within phoH at 1514.584 kb on + strand, within phoH at 1514.584 kb on + strand, within phoH
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 1,511,459 + pgaB MCAODC_23930 0.58 -0.1 1,511,459 + pgaB MCAODC_23930 0.58 +0.7 1,511,538 - pgaB MCAODC_23930 0.62 +1.0 1,511,639 + pgaB MCAODC_23930 0.67 -1.1 1,511,639 + pgaB MCAODC_23930 0.67 +1.1 1,511,678 + pgaB MCAODC_23930 0.69 +0.2 1,511,710 + pgaB MCAODC_23930 0.70 +0.4 1,512,337 - -0.5 1,512,360 + +1.7 1,512,524 - pgaC MCAODC_23935 0.17 +0.5 1,512,532 + pgaC MCAODC_23935 0.17 +0.4 1,512,827 + pgaC MCAODC_23935 0.39 +0.7 1,512,829 + pgaC MCAODC_23935 0.40 +1.0 1,512,829 + pgaC MCAODC_23935 0.40 -0.8 1,512,848 + pgaC MCAODC_23935 0.41 -0.4 1,512,901 + pgaC MCAODC_23935 0.45 -0.5 1,512,984 + pgaC MCAODC_23935 0.51 +1.7 1,513,081 + pgaC MCAODC_23935 0.59 -1.6 1,513,082 - pgaC MCAODC_23935 0.59 -0.6 1,513,143 + pgaC MCAODC_23935 0.63 -0.4 1,513,280 + pgaC MCAODC_23935 0.74 +0.9 1,513,378 + pgaC MCAODC_23935 0.81 -0.1 1,513,631 + -0.9 1,513,686 + pgaD MCAODC_23940 0.13 -1.5 1,513,687 - pgaD MCAODC_23940 0.13 +1.3 1,513,928 - pgaD MCAODC_23940 0.71 -1.7 1,513,934 - pgaD MCAODC_23940 0.73 -0.5 1,514,005 + -0.0 1,514,005 + -0.3 1,514,087 + -0.4 1,514,090 + -0.6 1,514,091 - +1.3 1,514,091 - -0.7 1,514,096 + +1.3 1,514,096 + -0.6 1,514,097 - +0.1 1,514,103 - -2.4 1,514,103 - +0.3 1,514,115 + -2.1 1,514,116 - -2.1 1,514,209 + phoH MCAODC_23945 0.11 +1.7 1,514,210 - phoH MCAODC_23945 0.11 -1.1 1,514,364 + phoH MCAODC_23945 0.25 -0.0 1,514,378 + phoH MCAODC_23945 0.27 +0.9 1,514,379 - phoH MCAODC_23945 0.27 -3.1 1,514,407 + phoH MCAODC_23945 0.29 +1.3 1,514,483 + phoH MCAODC_23945 0.36 -0.1 1,514,515 + phoH MCAODC_23945 0.39 +0.2 1,514,580 + phoH MCAODC_23945 0.46 -0.7 1,514,580 + phoH MCAODC_23945 0.46 +0.6 1,514,581 - phoH MCAODC_23945 0.46 +0.6 1,514,581 - phoH MCAODC_23945 0.46 -3.5 1,514,584 + phoH MCAODC_23945 0.46 +0.4 1,514,584 + phoH MCAODC_23945 0.46 +1.7 1,514,584 + phoH MCAODC_23945 0.46 +2.3
Or see this region's nucleotide sequence