Strain Fitness in Escherichia coli ECRC101 around MCAODC_22820

Experiment: WM_RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolE and lolD are separated by 5 nucleotideslolD and lolC overlap by 8 nucleotideslolC and ycfT are separated by 261 nucleotides MCAODC_22810: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 1,302,790 to 1,304,028 lolE MCAODC_22815: lolD - lipoprotein-releasing ABC transporter ATP-binding protein LolD, at 1,304,034 to 1,304,735 lolD MCAODC_22820: lolC - lipoprotein-releasing ABC transporter permease subunit LolC, at 1,304,728 to 1,305,927 lolC MCAODC_22825: ycfT - Inner membrane protein YcfT, at 1,306,189 to 1,307,262 ycfT Position (kb) 1304 1305 1306Strain fitness (log2 ratio) -2 -1 0 1 2at 1306.125 kb on + strandat 1306.139 kb on + strandat 1306.162 kb on + strandat 1306.163 kb on - strandat 1306.234 kb on - strandat 1306.234 kb on - strandat 1306.234 kb on - strandat 1306.234 kb on - strandat 1306.380 kb on + strand, within ycfTat 1306.406 kb on - strand, within ycfTat 1306.406 kb on - strand, within ycfTat 1306.624 kb on + strand, within ycfTat 1306.631 kb on - strand, within ycfTat 1306.638 kb on - strand, within ycfTat 1306.655 kb on + strand, within ycfTat 1306.703 kb on + strand, within ycfTat 1306.778 kb on - strand, within ycfTat 1306.811 kb on + strand, within ycfT

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_RB68
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1,306,125 + -0.3
1,306,139 + +0.4
1,306,162 + -0.1
1,306,163 - -0.7
1,306,234 - -0.8
1,306,234 - +0.8
1,306,234 - +0.2
1,306,234 - +2.1
1,306,380 + ycfT MCAODC_22825 0.18 +0.0
1,306,406 - ycfT MCAODC_22825 0.20 -1.8
1,306,406 - ycfT MCAODC_22825 0.20 -1.0
1,306,624 + ycfT MCAODC_22825 0.41 +1.3
1,306,631 - ycfT MCAODC_22825 0.41 +2.7
1,306,638 - ycfT MCAODC_22825 0.42 +0.1
1,306,655 + ycfT MCAODC_22825 0.43 -0.3
1,306,703 + ycfT MCAODC_22825 0.48 +1.3
1,306,778 - ycfT MCAODC_22825 0.55 -1.0
1,306,811 + ycfT MCAODC_22825 0.58 -1.4

Or see this region's nucleotide sequence