Strain Fitness in Escherichia coli ECRC101 around MCAODC_20345

Experiment: WM_RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeS and yeeT are separated by 62 nucleotidesyeeT and cbtA are separated by 73 nucleotidescbtA and MCAODC_20345 are separated by 88 nucleotidesMCAODC_20345 and yeeW are separated by 106 nucleotides MCAODC_20330: yeeS - UPF0758 protein YeeS, at 876,675 to 877,151 yeeS MCAODC_20335: yeeT - Uncharacterized protein YeeT, at 877,214 to 877,435 yeeT MCAODC_20340: cbtA - type IV toxin-antitoxin system toxin CbtA, at 877,509 to 877,877 cbtA MCAODC_20345: MCAODC_20345 - toxin of the YeeV-YeeU toxin-antitoxin system, at 877,966 to 878,229 _20345 MCAODC_20350: yeeW - Protein YeeW, at 878,336 to 878,530 yeeW Position (kb) 877 878 879Strain fitness (log2 ratio) -2 -1 0 1 2 3at 877.186 kb on + strandat 877.404 kb on - strand, within yeeTat 877.404 kb on - strand, within yeeTat 877.469 kb on - strandat 877.469 kb on - strandat 877.937 kb on + strandat 877.937 kb on + strandat 877.939 kb on + strandat 877.982 kb on + strandat 877.983 kb on - strandat 877.983 kb on - strandat 877.985 kb on + strandat 878.036 kb on + strand, within MCAODC_20345at 878.036 kb on + strand, within MCAODC_20345at 878.037 kb on - strand, within MCAODC_20345at 878.065 kb on + strand, within MCAODC_20345at 878.318 kb on + strandat 878.318 kb on + strandat 878.367 kb on + strand, within yeeWat 878.413 kb on + strand, within yeeWat 878.413 kb on + strand, within yeeWat 878.416 kb on + strand, within yeeWat 878.416 kb on + strand, within yeeWat 878.431 kb on + strand, within yeeWat 878.432 kb on - strand, within yeeWat 878.434 kb on + strand, within yeeWat 878.434 kb on + strand, within yeeWat 878.434 kb on + strand, within yeeWat 878.504 kb on + strand, within yeeWat 878.504 kb on + strand, within yeeWat 878.505 kb on - strand, within yeeWat 878.505 kb on - strand, within yeeWat 878.505 kb on - strand, within yeeWat 878.528 kb on + strandat 878.529 kb on - strandat 878.551 kb on + strandat 878.552 kb on - strandat 878.553 kb on + strandat 878.554 kb on - strandat 878.593 kb on + strandat 878.593 kb on + strandat 878.720 kb on + strandat 878.778 kb on - strandat 878.782 kb on + strandat 878.782 kb on + strandat 878.783 kb on - strandat 878.785 kb on + strandat 878.785 kb on + strandat 878.832 kb on + strandat 878.833 kb on - strandat 878.963 kb on + strandat 878.963 kb on + strandat 879.119 kb on + strandat 879.170 kb on + strandat 879.171 kb on - strandat 879.204 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_RB68
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877,186 + +0.2
877,404 - yeeT MCAODC_20335 0.86 -0.4
877,404 - yeeT MCAODC_20335 0.86 +0.5
877,469 - -0.4
877,469 - -1.2
877,937 + +0.8
877,937 + -0.1
877,939 + -0.5
877,982 + +0.5
877,983 - -1.1
877,983 - -0.7
877,985 + -0.0
878,036 + MCAODC_20345 0.27 +0.2
878,036 + MCAODC_20345 0.27 +0.7
878,037 - MCAODC_20345 0.27 +0.3
878,065 + MCAODC_20345 0.38 -0.1
878,318 + +0.6
878,318 + +0.3
878,367 + yeeW MCAODC_20350 0.16 -0.8
878,413 + yeeW MCAODC_20350 0.39 -0.3
878,413 + yeeW MCAODC_20350 0.39 +0.8
878,416 + yeeW MCAODC_20350 0.41 +0.0
878,416 + yeeW MCAODC_20350 0.41 +2.2
878,431 + yeeW MCAODC_20350 0.49 +2.1
878,432 - yeeW MCAODC_20350 0.49 +1.0
878,434 + yeeW MCAODC_20350 0.50 +0.1
878,434 + yeeW MCAODC_20350 0.50 +1.6
878,434 + yeeW MCAODC_20350 0.50 +0.1
878,504 + yeeW MCAODC_20350 0.86 -0.5
878,504 + yeeW MCAODC_20350 0.86 +0.0
878,505 - yeeW MCAODC_20350 0.87 -1.7
878,505 - yeeW MCAODC_20350 0.87 -0.3
878,505 - yeeW MCAODC_20350 0.87 -1.8
878,528 + +0.1
878,529 - -0.1
878,551 + +1.2
878,552 - -0.9
878,553 + -1.7
878,554 - -0.3
878,593 + -1.3
878,593 + +0.5
878,720 + +0.4
878,778 - -0.2
878,782 + -0.6
878,782 + +0.6
878,783 - -0.3
878,785 + -0.7
878,785 + +0.0
878,832 + +1.1
878,833 - +2.8
878,963 + -0.1
878,963 + +0.4
879,119 + +0.0
879,170 + -0.4
879,171 - +1.4
879,204 - +1.1

Or see this region's nucleotide sequence