Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumD and pykF are separated by 556 nucleotides pykF and lpp are separated by 310 nucleotides lpp and ldtE are separated by 62 nucleotides
MCAODC_18115: fumD - fumarate hydratase FumD, at 483,913 to 484,122
fumD
MCAODC_18120: pykF - pyruvate kinase PykF, at 484,679 to 486,091
pykF
MCAODC_18125: lpp - murein lipoprotein Lpp, at 486,402 to 486,638
lpp
MCAODC_18130: ldtE - L,D-transpeptidase LdtE, at 486,701 to 487,705
ldtE
Position (kb) 484
485
486
487 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 483.920 kb on - strand at 483.997 kb on - strand, within fumD at 484.000 kb on + strand, within fumD at 484.001 kb on - strand, within fumD at 484.021 kb on - strand, within fumD at 484.021 kb on - strand, within fumD at 484.021 kb on - strand, within fumD at 484.138 kb on - strand at 484.150 kb on - strand at 484.159 kb on + strand at 484.159 kb on + strand at 484.160 kb on - strand at 484.160 kb on - strand at 484.222 kb on + strand at 484.247 kb on + strand at 484.248 kb on - strand at 484.254 kb on - strand at 484.274 kb on + strand at 484.351 kb on + strand at 484.363 kb on - strand at 484.378 kb on - strand at 486.024 kb on + strand at 486.064 kb on + strand at 486.089 kb on + strand at 486.090 kb on - strand at 486.194 kb on - strand at 486.196 kb on - strand at 486.196 kb on - strand at 486.198 kb on - strand at 486.261 kb on - strand at 486.366 kb on + strand at 486.446 kb on + strand, within lpp at 486.623 kb on + strand at 486.623 kb on + strand at 486.623 kb on + strand at 486.623 kb on + strand at 486.636 kb on + strand at 486.636 kb on + strand at 486.636 kb on + strand at 486.636 kb on + strand at 486.639 kb on + strand at 486.642 kb on + strand at 486.697 kb on - strand at 486.721 kb on + strand at 486.759 kb on - strand at 486.857 kb on + strand, within ldtE at 486.866 kb on - strand, within ldtE at 487.078 kb on + strand, within ldtE
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 483,920 - +0.5 483,997 - fumD MCAODC_18115 0.40 +0.3 484,000 + fumD MCAODC_18115 0.41 +2.1 484,001 - fumD MCAODC_18115 0.42 +0.3 484,021 - fumD MCAODC_18115 0.51 -0.4 484,021 - fumD MCAODC_18115 0.51 -2.3 484,021 - fumD MCAODC_18115 0.51 -1.0 484,138 - -1.2 484,150 - -1.6 484,159 + -0.4 484,159 + +0.2 484,160 - +0.3 484,160 - +0.3 484,222 + -0.3 484,247 + +0.7 484,248 - +0.5 484,254 - -0.2 484,274 + +0.6 484,351 + +2.8 484,363 - -1.2 484,378 - +0.8 486,024 + +0.2 486,064 + -1.0 486,089 + -0.0 486,090 - +0.3 486,194 - -1.6 486,196 - +0.9 486,196 - -0.1 486,198 - +0.1 486,261 - -0.7 486,366 + +1.7 486,446 + lpp MCAODC_18125 0.19 +0.8 486,623 + -0.6 486,623 + -0.1 486,623 + +1.4 486,623 + -0.2 486,636 + +0.8 486,636 + +0.8 486,636 + +0.3 486,636 + +0.6 486,639 + +2.1 486,642 + +0.8 486,697 - -1.8 486,721 + -2.6 486,759 - +0.8 486,857 + ldtE MCAODC_18130 0.16 -0.9 486,866 - ldtE MCAODC_18130 0.16 +1.3 487,078 + ldtE MCAODC_18130 0.38 +0.2
Or see this region's nucleotide sequence